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SLC26A5 Antibody (PA5-103158) in ICC/IF
Immunocytochemistry analysis of SLC26A5 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SLC26A5 polyclonal antibody (Product # PA5-103158) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103158
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SLC26A5(Accession P58743), corresponding to amino acid residues A427-V477.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852528
Product Specific Information
Antibody detects endogenous levels of total Prestin.
Target Information
SLC26A5 is a member of the SLC26A/SulP transporter family. SLC26A5 is specifically expressed in outer hair cells (OHCs) of the cochlea and is essential in auditory processing. Intracellular anions are thought to act as extrinsic voltage sensors, which bind to this protein and trigger the conformational changes required for rapid length changes in OHCs. Mutations in its gene have been associated with non-syndromic hearing loss.This gene is a member of the SLC26A/SulP transporter family. It encodes a protein that is specifically expressed in outer hair cells (OHCs) of the cochlea and is essential in auditory processing. Intracellular anions are thought to act as extrinsic voltage sensors, which bind to this protein and trigger the conformational changes required for rapid length changes in OHCs. Mutations in this gene have been associated with non-syndromic hearing loss. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC118886; MGC118887; MGC118888; MGC118889; outer hair cell motor protein; Prestin; prestin (motor protein); solute carrier family 26 (anion exchanger), member 5; Solute carrier family 26 member 5; solute carrier family 26, member 5 (prestin)
Gene Aliases: DFNB61; PRES; prestin; SLC26A5
UniProt ID: (Human) P58743, (Mouse) Q99NH7, (Rat) Q9EPH0
Entrez Gene ID: (Human) 375611, (Mouse) 80979, (Rat) 83819
chloride channel activity
transcription factor binding
secondary active sulfate transmembrane transporter activity
bicarbonate transmembrane transporter activity
sulfate transmembrane transporter activity
anion:anion antiporter activity
oxalate transmembrane transporter activity
spectrin binding
protein homodimerization activity
protein binding
motor activity
transporter
response to ischemia
sensory perception of sound
regulation of cell shape
response to salicylic acid
response to auditory stimulus
bicarbonate transport
fructose transport
oxalate transport
negative regulation of ion transmembrane transport
response to potassium ion
regulation of membrane potential
response to drug
positive regulation of cell size
protein tetramerization
regulation of intracellular pH
cochlea development
response to thyroid hormone
response to salt
sulfate transmembrane transport
chloride transmembrane transport
positive regulation of cell motility
sulfate transport
ion transmembrane transport
transmembrane transport
anion transmembrane transport
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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