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SLC28A1 Antibody (PA5-104163) in ICC/IF
Immunocytochemistry analysis of SLC28A1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SLC28A1 polyclonal antibody (Product # PA5-104163) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-104163
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SLC28A1(Accession O00337), corresponding to amino acid residues G20-R70.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853491
Product Specific Information
Antibody detects endogenous levels of total SLC28A1.
Target Information
Nucleosides play a role in signaling in several physiologic systems, and synthetic analogs of natural nucleosides are often used to treat neoplastic and viral diseases. Plasma membrane transport of nucleosides is mediated by equilibrative and concentrative nucleoside transporters, which may have specificity for purines or pyrimidines. The deduced human 650 amino acid concentrative nucleoside transporter 1 (CNT1) protein is 83% identical to the rat protein and is expressed in the intestine, kidney and liver. CNT1, also designated solute carrier family 28 (sodium-coupled nucleoside transporter), member 1 (SLC28A1), expedites sodium-dependent fluxes of uridine, azidodeoxythymidine (AZT) and adenosine, but not of guanosine or deoxyadenosine, which undergo net renal secretion. CNT1 activity may serve as a putative mechanism for renal reabsorption of physiologic nucleosides and synthetic nucleoside drugs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CNT 1; Concentrative nucleoside transporter 1; Na(+)/nucleoside cotransporter 1; Sodium-coupled nucleoside transporter 1; Sodium/nucleoside cotransporter 1; solute carrier family 28 (concentrative nucleoside transporter), member 1; solute carrier family 28 (sodium-coupled nucleoside transporter), member 1; Solute carrier family 28 member 1; solute carrier family 28, member 1
Gene Aliases: CNT1; HCNT1; SLC28A1
UniProt ID: (Human) O00337, (Rat) Q62674, (Mouse) E9PXX9
Entrez Gene ID: (Human) 9154, (Rat) 116642, (Mouse) 434203
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