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SLC7A11 Antibody (MA5-50633) in ICC/IF
Immunocytochemistry analysis of SLC7A11 in HT-29 cells. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were incubated with SLC7A11 recombinant monoclonal antibody (Product # MA5-50633) with a dilution of 1:200 in 2% BSA overnight at 4 . Followed by secondary antibody Goat Anti-Mouse IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the primary antibody was used as the secondary antibody on... View More
Product Details
MA5-50633
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2b
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
A7C6-R
Immunogen
293 cell line overexpressing SLC7A11.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3093725
Product Specific Information
Positive control: HT-29 cell lysate, A549 cell lysate, HCT116 cell lysates, human pancreas tissue, HT-29, A549, NIH/3T3 cell lysates.
Predicted band size: 55 kDa
Subcellular Location: Membrane.
Target Information
SLC7A11 (also known as XCT) is a member of a hetromeric, sodium-independent, anionic amino acid transport system. It is highly specific for cysteine and glutamate. SLC7A11 is a mediator of Kaposi sarcoma-associated herpes virus fusion and entry into cells. Increased expression of this protein in primary gliomas is associated with neuronal cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amino acid transport system xc-; Calcium channel blocker resistance protein CCBR1; cysteine/glutamate transporter; Cystine/glutamate transporter; sodium independent anionic amino acid transport system; solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11; Solute carrier family 7 member 11; solute carrier family 7, (cationic amino acid transporter, y+ system) member 11; Subtle gray protein; xCT
Gene Aliases: 9930009M05Rik; AI451155; CCBR1; SLC7A11; sut; xCT
UniProt ID: (Human) Q9UPY5, (Mouse) Q9WTR6
Entrez Gene ID: (Human) 23657, (Mouse) 26570
amino acid transmembrane transport
amino acid transport
response to oxidative stress
brain development
response to toxic substance
response to nicotine
leukocyte migration
lens fiber cell differentiation
platelet aggregation
L-glutamate transmembrane transport
transport
neutral amino acid transport
modified amino acid transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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