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SLIT2 Polyclonal Antibody

SLIT2 Antibody in Immunocytochemistry (ICC/IF)

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SLIT2 Antibody (20217-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HEK-293 cells using 20217-1-AP (SLIT2-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SLIT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

20217-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 4 publications 4 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to SLIT2.

Target Information

SLIT2 are ligands for Robo receptors and play a vital role for axonal migration/navigation during neural development. A number of cleavage products through alternate splicing are reported in the literature for SLIT2 proteins. The C-terminal cleavage proteins are more diffusible than the larger N-terminal protein that is more tightly cell associated. SLIT2 protein is expressed in tissues such as fetal lung, kidney, and adult spinal cord.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PIEZO2 promotes cell proliferation and metastasis in colon carcinoma through the SLIT2/ROBO1/VEGFC pathway.

Advances in clinical and experimental medicine : official organ Wroclaw Medical University

Shang H,Xu A,Yan H,Xu D,Zhang J,Fang X

Sat Jul 01 00:00:00 EDT 2023

CCN2/CTGF promotes liver fibrosis through crosstalk with the Slit2/Robo signaling.

Journal of cell communication and signaling

Pi L,Sun C,Jn-Simon N,Basha S,Thomas H,Figueroa V,Zarrinpar A,Cao Q,Petersen B

Wed Mar 01 00:00:00 EST 2023

CCN2/CTGF promotes liver fibrosis through crosstalk with the Slit2/Robo signaling.

Journal of cell communication and signaling

Pi L,Sun C,Jn-Simon N,Basha S,Thomas H,Figueroa V,Zarrinpar A,Cao Q,Petersen B

Wed Mar 01 00:00:00 EST 2023

An integrated single cell and spatial transcriptomic map of human white adipose tissue.

Nature communications

Massier L,Jalkanen J,Elmastas M,Zhong J,Wang T,Nono Nankam PA,Frendo-Cumbo S,Bäckdahl J,Subramanian N,Sekine T,Kerr AG,Tseng BTP,Laurencikiene J,Buggert M,Lourda M,Kublickiene K,Bhalla N,Andersson A,Valsesia A,Astrup A,Blaak EE,Ståhl PL,Viguerie N,Langin D,Wolfrum C,Blüher M,Rydén M,Mejhert N

Wed Mar 15 00:00:00 EDT 2023

RNA m6A reader IGF2BP3 promotes metastasis of triple-negative breast cancer via SLIT2 repression.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Jiang T,He X,Zhao Z,Zhang X,Wang T,Jia L

Tue Nov 01 00:00:00 EDT 2022

RNA m6A reader IGF2BP3 promotes metastasis of triple-negative breast cancer via SLIT2 repression.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Jiang T,He X,Zhao Z,Zhang X,Wang T,Jia L

Tue Nov 01 00:00:00 EDT 2022

Role of Slit2 upregulation in recurrent miscarriage through regulation of stromal decidualization.

Placenta

Chen J,Liu J,Gao S,Qiu Y,Wang Y,Zhang Y,Gao L,Qi G,Wu Y,Lash GE,Li P

Fri Jan 01 00:00:00 EST 2021

Mechanistic Targets of Diallyl Trisulfide in Human Breast Cancer Cells Identified by RNA-seq Analysis.

Journal of cancer prevention

Hahm ER,Kim SH,Mathan SV,Singh RP,Singh SV

Wed Jun 30 00:00:00 EDT 2021

Crosstalk between the activated Slit2-Robo1 pathway and TGF-β1 signalling promotes cardiac fibrosis.

ESC heart failure

Liu Y,Yin Z,Xu X,Liu C,Duan X,Song Q,Tuo Y,Wang C,Yang J,Yin S

Mon Feb 01 00:00:00 EST 2021

Crosstalk between the activated Slit2-Robo1 pathway and TGF-β1 signalling promotes cardiac fibrosis.

ESC heart failure

Liu Y,Yin Z,Xu X,Liu C,Duan X,Song Q,Tuo Y,Wang C,Yang J,Yin S

Mon Feb 01 00:00:00 EST 2021

Synaptogenic activity of the axon guidance molecule Robo2 underlies hippocampal circuit function.

Cell reports

Blockus H,Rolotti SV,Szoboszlay M,Peze-Heidsieck E,Ming T,Schroeder A,Apostolo N,Vennekens KM,Katsamba PS,Bahna F,Mannepalli S,Ahlsen G,Honig B,Shapiro L,de Wit J,Losonczy A,Polleux F

Tue Oct 19 00:00:00 EDT 2021

Synaptogenic activity of the axon guidance molecule Robo2 underlies hippocampal circuit function.

Cell reports

Blockus H,Rolotti SV,Szoboszlay M,Peze-Heidsieck E,Ming T,Schroeder A,Apostolo N,Vennekens KM,Katsamba PS,Bahna F,Mannepalli S,Ahlsen G,Honig B,Shapiro L,de Wit J,Losonczy A,Polleux F

Tue Oct 19 00:00:00 EDT 2021

Identification and validation of smoking-related genes in lung adenocarcinoma using an in vitro carcinogenesis model and bioinformatics analysis.

Journal of translational medicine

Wang J,Chen T,Yu X,OUYang N,Tan L,Jia B,Tong J,Li J

Fri Aug 14 00:00:00 EDT 2020

Deletion of Mea6 in Cerebellar Granule Cells Impairs Synaptic Development and Motor Performance.

Frontiers in cell and developmental biology

Wang XT,Zhou L,Cai XY,Xu FX,Xu ZH,Li XY,Shen Y

Tue Mar 16 00:00:00 EDT 2021

Depression-/Anxiety-Like Behavior Alterations in Adult Slit2 Transgenic Mice.

Frontiers in behavioral neuroscience

Huang G,Wang S,Yan J,Li C,Feng J,Chen Q,Zheng X,Li H,He Y,Young AJ,Li H,Li W,Li J,Wang L

Tue Feb 23 00:00:00 EST 2021

Tumoural activation of TLR3-SLIT2 axis in endothelium drives metastasis.

Nature

Tavora B,Mederer T,Wessel KJ,Ruffing S,Sadjadi M,Missmahl M,Ostendorf BN,Liu X,Kim JY,Olsen O,Welm AL,Goodarzi H,Tavazoie SF

Thu Oct 01 00:00:00 EDT 2020

Tumoural activation of TLR3-SLIT2 axis in endothelium drives metastasis.

Nature

Tavora B,Mederer T,Wessel KJ,Ruffing S,Sadjadi M,Missmahl M,Ostendorf BN,Liu X,Kim JY,Olsen O,Welm AL,Goodarzi H,Tavazoie SF

Thu Oct 01 00:00:00 EDT 2020

Tumoural activation of TLR3-SLIT2 axis in endothelium drives metastasis.

Nature

Tavora B,Mederer T,Wessel KJ,Ruffing S,Sadjadi M,Missmahl M,Ostendorf BN,Liu X,Kim JY,Olsen O,Welm AL,Goodarzi H,Tavazoie SF

Thu Oct 01 00:00:00 EDT 2020

Tumoural activation of TLR3-SLIT2 axis in endothelium drives metastasis.

Nature

Tavora B,Mederer T,Wessel KJ,Ruffing S,Sadjadi M,Missmahl M,Ostendorf BN,Liu X,Kim JY,Olsen O,Welm AL,Goodarzi H,Tavazoie SF

Thu Oct 01 00:00:00 EDT 2020

Inhibition of miR-200b-3p alleviates hypoxia-ischemic brain damage via targeting Slit2 in neonatal rats.

Biochemical and biophysical research communications

Zhang N,Yang L,Meng L,Cui H

Thu Mar 19 00:00:00 EDT 2020

MEA6 Deficiency Impairs Cerebellar Development and Motor Performance by Tethering Protein Trafficking.

Frontiers in cellular neuroscience

Wang XT,Cai XY,Xu FX,Zhou L,Zheng R,Ma KY,Xu ZH,Shen Y

Thu Oct 01 00:00:00 EDT 2020

Slit2/Robo1 signaling is involved in angiogenesis of glomerular endothelial cells exposed to a diabetic-like environment.

Angiogenesis

Liu J,Hou W,Guan T,Tang L,Zhu X,Li Y,Hou S,Zhang J,Chen H,Huang Y

Tue May 01 00:00:00 EDT 2018

Slit2/Robo1 signaling is involved in angiogenesis of glomerular endothelial cells exposed to a diabetic-like environment.

Angiogenesis

Liu J,Hou W,Guan T,Tang L,Zhu X,Li Y,Hou S,Zhang J,Chen H,Huang Y

Tue May 01 00:00:00 EDT 2018

Alterted SLIT2/ROBO1 signalling is linked to impaired placentation of missed and threatened miscarriage in early pregnancy.

Histopathology

Li P,Shi Y,Shuai H,Cai Y,Lu W,Wang G,Gao L,Wang L,Fan X,Yang X

Sun Oct 01 00:00:00 EDT 2017

Alterted SLIT2/ROBO1 signalling is linked to impaired placentation of missed and threatened miscarriage in early pregnancy.

Histopathology

Li P,Shi Y,Shuai H,Cai Y,Lu W,Wang G,Gao L,Wang L,Fan X,Yang X

Sun Oct 01 00:00:00 EDT 2017

Alterted SLIT2/ROBO1 signalling is linked to impaired placentation of missed and threatened miscarriage in early pregnancy.

Histopathology

Li P,Shi Y,Shuai H,Cai Y,Lu W,Wang G,Gao L,Wang L,Fan X,Yang X

Sun Oct 01 00:00:00 EDT 2017

Alterted SLIT2/ROBO1 signalling is linked to impaired placentation of missed and threatened miscarriage in early pregnancy.

Histopathology

Li P,Shi Y,Shuai H,Cai Y,Lu W,Wang G,Gao L,Wang L,Fan X,Yang X

Sun Oct 01 00:00:00 EDT 2017

Dendrite self-avoidance requires cell-autonomous slit/robo signaling in cerebellar purkinje cells.

Neuron

Gibson DA,Tymanskyj S,Yuan RC,Leung HC,Lefebvre JL,Sanes JR,Chédotal A,Ma L

Wed Mar 05 00:00:00 EST 2014

Dendrite self-avoidance requires cell-autonomous slit/robo signaling in cerebellar purkinje cells.

Neuron

Gibson DA,Tymanskyj S,Yuan RC,Leung HC,Lefebvre JL,Sanes JR,Chédotal A,Ma L

Wed Mar 05 00:00:00 EST 2014

Bioinformatics

Protein Aliases: downregulated during adipocyte differentiation-1; neurogenic extracellular slit protein; Slit homolog 2 protein; Slit-2

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Gene Aliases: Drad-1; E030015M03Rik; E130320P19Rik; mKIAA4141; SLIL3; Slit-2; SLIT2

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UniProt ID: (Human) O94813, (Mouse) Q9R1B9

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Entrez Gene ID: (Human) 9353, (Rat) 360272, (Mouse) 20563

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Function(s)

GTPase inhibitor activity calcium ion binding protein binding heparin binding identical protein binding protein homodimerization activity laminin-1 binding proteoglycan binding heparan sulfate proteoglycan binding Roundabout binding follicle-stimulating hormone receptor activity chemorepellent activity receptor binding immunoglobulin superfamily cell adhesion molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SLIT2 Polyclonal Antibody

SLIT2 Antibody (20217-1-AP) in ICC/IF

Product Details

20217-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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