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SLIT3 Antibody (600-401-884) in WB
Western blot using Rocklands Affinity Purified anti-SLIT-3 antibody shows detection of a predominant band at ~145 kDa corresponding to SLIT-3 (arrowhead) in a bovine thyroid whole cell lysate using the 800 nm channel (green). ~ 35 µg of lysate was separated on a 4-8% Tricine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:800. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Ra... View More
Product Details
600-401-884
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 1155-1180 of Human SLIT-3 protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human SLIT-3 protein. A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, bovine and mouse based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-3 from chimpanzee and chicken sources, as only a single amino acid residue change is found within the immunogen sequence (93% positive by BLAST). Cross reactivity with SLIT-3 homologues from other sources has not been determined.
Target Information
SLIT3 may act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Multiple EGF-like domains protein 5; multiple epidermal growth factor-like domains 5; Multiple epidermal growth factor-like domains protein 5; SLIL2FLJ10764; slit homolog 3; Slit homolog 3 protein; Slit-3
Gene Aliases: b2b2362.1Clo; KIAA0814; MEGF5; SLIL2; Slit-3; SLIT1; slit2; SLIT3; UNQ691/PRO1336
UniProt ID: (Human) O75094, (Rat) O88280, (Mouse) Q9WVB4
Entrez Gene ID: (Dog) 489132, (Bovine) 615883, (Human) 6586, (Rat) 83467, (Mouse) 20564
axon guidance
negative regulation of cell proliferation
organ morphogenesis
negative regulation of gene expression
chemorepulsion involved in embryonic olfactory bulb interneuron precursor migration
negative regulation of cell growth
cellular response to hormone stimulus
Roundabout signaling pathway
axon extension involved in axon guidance
regulation of axonogenesis
negative chemotaxis
response to cortisol
apoptotic process involved in luteolysis
negative regulation of chemokine-mediated signaling pathway
brain development
spinal cord development
cell differentiation
multicellular organism development
nervous system development
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