Invitrogen

SMAD1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

30 Published Figures

21 References

View all (59) SMAD1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

SMAD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 35

SMAD1 Antibody (38-5400) in IHC (P)

Immunohistochemistry analysis of SMAD1 showing staining in the cytoplasm of paraffin-embedded mouse heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, was... View More

SMAD1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

SMAD1 Antibody in Immunohistochemistry (IHC)

SMAD1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

SMAD1 Antibody — Fig 1 TGF-beta inhibits BMP-induced transcriptional responses in an ALK5-dependent manner. (A, B, and E to G) MDA-MB-231 BRE cells and MDA-MB-231 CAGA cells were induced with the ligands indicated and assayed for luciferase and Renilla activities after 8 h. (C) C2C12 and HaCaT cells were transiently transfected with BRE-luciferase and TK- Renilla . After 24 h, the C2C12 cells were induced with the ligands shown and luciferase/ Renilla assays were performed. At 24 h after transfection, HaCaT cells were starved overnight in Opti-MEM before ligand induction. (D) MDA-MB-231 cells were transfected with ID2-luciferase and TK- Renilla for 24 h prior to ligand induction. In panel F, SB-431542 was added 15 min before the ligands shown, and in panel G, either dimethyl sulfoxide (DMSO) or SB-431542 was added 15, 30, or 60 min after ligand addition, as shown. All experiments were performed in triplicate. The data are presented as luciferase activity normalized to Renilla activity and are the mean and standard deviation from a single representative experiment. In panel E (right side), in parallel with the luciferase assay, MDA-MB-231 BRE cells were treated with ligands as indicated for 1 h. Whole-cell extracts were Western blotted using antibodies against PSmad1/5, PSmad2, and PSmad3, as well as total Smad1, -2, and -3. MCM7 is a loading control. The asterisk indicates a nonspecific band. {TM}

Product Details

38-5400

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 2 publications 2 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human, Mouse, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from an internal region of the human Smad1 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533373

Target Information

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Essential role of the amino-terminal region of Drosha for the Microprocessor function.

iScience

Prabhakar A,Hu S,Tang J,Ghatpande P,Lagna G,Jiang X,Hata A

38-5400 was used in Western Blot to reveal the essential role of Drosha-NTR in miRNA production and nutrient-dependent translational control.

Fri Oct 20 00:00:00 EDT 2023

Pinhead antagonizes Admp to promote notochord formation.

iScience

Itoh K,Ossipova O,Sokol SY

38-5400 was used in Western Blot to evaluate the molecular targets and signaling mechanisms of the Pinhead protein during Xenopus gastrulation.

Fri Jun 25 00:00:00 EDT 2021

Pinhead antagonizes Admp to promote notochord formation.

iScience

Itoh K,Ossipova O,Sokol SY

38-5400 was used in Western Blot to evaluate the molecular targets and signaling mechanisms of the Pinhead protein during Xenopus gastrulation.

Fri Jun 25 00:00:00 EDT 2021

Pinhead signaling regulates mesoderm heterogeneity via the FGF receptor-dependent pathway.

Development (Cambridge, England)

Ossipova O,Itoh K,Radu A,Ezan J,Sokol SY

38-5400 was used in Immunoprecipitation to propose that during gastrulation Pnhd acts in the marginal zone to contribute to mesoderm heterogeneity via an FGF receptor-dependent positive feedback mechanism.

Fri Sep 11 00:00:00 EDT 2020

Bone morphogenetic protein 7 promotes resistance to immunotherapy.

Nature communications

Cortez MA,Masrorpour F,Ivan C,Zhang J,Younes AI,Lu Y,Estecio MR,Barsoumian HB,Menon H,Caetano MDS,Ramapriyan R,Schoenhals JE,Wang X,Skoulidis F,Wasley MD,Calin G,Hwu P,Welsh JW

38-5400 was used in Immunohistochemistry to identify the BMP7 signaling pathway as a potential immunotherapeutic target in cancer.

Thu Sep 24 00:00:00 EDT 2020

Bone morphogenetic protein 7 promotes resistance to immunotherapy.

Nature communications

Cortez MA,Masrorpour F,Ivan C,Zhang J,Younes AI,Lu Y,Estecio MR,Barsoumian HB,Menon H,Caetano MDS,Ramapriyan R,Schoenhals JE,Wang X,Skoulidis F,Wasley MD,Calin G,Hwu P,Welsh JW

38-5400 was used in Immunohistochemistry to identify the BMP7 signaling pathway as a potential immunotherapeutic target in cancer.

Thu Sep 24 00:00:00 EDT 2020

TGF-β family ligands exhibit distinct signalling dynamics that are driven by receptor localisation.

Journal of cell science

Miller DSJ,Schmierer B,Hill CS

38-5400 was used in Western Blotting to provide a clear rationale for the different mechanisms of pathway regulation found in vivo for each of these growth factors.

Mon Jul 15 00:00:00 EDT 2019

The Dynamics of TGF-β Signaling Are Dictated by Receptor Trafficking via the ESCRT Machinery.

Cell reports

Miller DSJ,Bloxham RD,Jiang M,Gori I,Saunders RE,Das D,Chakravarty P,Howell M,Hill CS

38-5400 was used in Western Blotting to identify the endosomal sorting complex required for transport machinery as a crucial determinant of transforming growth factor Β signal duration.

Tue Nov 13 00:00:00 EST 2018

Genomic integration of Wnt/β-catenin and BMP/Smad1 signaling coordinates foregut and hindgut transcriptional programs.

Development (Cambridge, England)

Stevens ML,Chaturvedi P,Rankin SA,Macdonald M,Jagannathan S,Yukawa M,Barski A,Zorn AM

38-5400 was used in Chromatin immunoprecipitation to identify the transcriptomes of Xenopus foregut and hindgut progenitors, which are conserved with mammals.

Sat Apr 01 00:00:00 EDT 2017

ZC4H2 stabilizes Smads to enhance BMP signalling, which is involved in neural development in Xenopus.

Open biology

Ma P,Ren B,Yang X,Sun B,Liu X,Kong Q,Li C,Mao B

Published figure using SMAD1 polyclonal antibody (Product # 38-5400) in Immunoprecipitation

Tue Aug 01 00:00:00 EDT 2017

ZC4H2 stabilizes Smads to enhance BMP signalling, which is involved in neural development in Xenopus.

Open biology

Ma P,Ren B,Yang X,Sun B,Liu X,Kong Q,Li C,Mao B

Published figure using SMAD1 polyclonal antibody (Product # 38-5400) in Immunoprecipitation

Tue Aug 01 00:00:00 EDT 2017

Chordin-Like 1 Suppresses Bone Morphogenetic Protein 4-Induced Breast Cancer Cell Migration and Invasion.

Molecular and cellular biology

Cyr-Depauw C,Northey JJ,Tabariès S,Annis MG,Dong Z,Cory S,Hallett M,Rennhack JP,Andrechek ER,Siegel PM

Published figure using SMAD1 polyclonal antibody (Product # 38-5400) in Western Blot

Sun May 15 00:00:00 EDT 2016

BMPR1A antagonist differentially affects cartilage and bone formation during fracture healing.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Morgan EF,Pittman J,DeGiacomo A,Cusher D,de Bakker CM,Mroszczyk KA,Grinstaff MW,Gerstenfeld LC

385400 was used in immunohistochemistry to assess the effects of soluble BMP receptor type 1A on secondary fracture healing

Thu Dec 01 00:00:00 EST 2016

The Proto-oncogene Transcription Factor Ets1 Regulates Neural Crest Development through Histone Deacetylase 1 to Mediate Output of Bone Morphogenetic Protein Signaling.

The Journal of biological chemistry

Wang C,Kam RK,Shi W,Xia Y,Chen X,Cao Y,Sun J,Du Y,Lu G,Chen Z,Chan WY,Chan SO,Deng Y,Zhao H

Published figure using SMAD1 polyclonal antibody (Product # 38-5400) in Western Blot

Fri Sep 04 00:00:00 EDT 2015

Pyruvate Dehydrogenase Kinase 4 Promotes Vascular Calcification via SMAD1/5/8 Phosphorylation.

Scientific reports

Lee SJ,Jeong JY,Oh CJ,Park S,Kim JY,Kim HJ,Doo Kim N,Choi YK,Do JY,Go Y,Ha CM,Choi JY,Huh S,Ho Jeoung N,Lee KU,Choi HS,Wang Y,Park KG,Harris RA,Lee IK

Published figure using SMAD1 polyclonal antibody (Product # 38-5400) in Western Blot

Thu Nov 12 00:00:00 EST 2015

Pyruvate Dehydrogenase Kinase 4 Promotes Vascular Calcification via SMAD1/5/8 Phosphorylation.

Scientific reports

Lee SJ,Jeong JY,Oh CJ,Park S,Kim JY,Kim HJ,Doo Kim N,Choi YK,Do JY,Go Y,Ha CM,Choi JY,Huh S,Ho Jeoung N,Lee KU,Choi HS,Wang Y,Park KG,Harris RA,Lee IK

Published figure using SMAD1 polyclonal antibody (Product # 38-5400) in Western Blot

Thu Nov 12 00:00:00 EST 2015

Pyruvate Dehydrogenase Kinase 4 Promotes Vascular Calcification via SMAD1/5/8 Phosphorylation.

Scientific reports

Lee SJ,Jeong JY,Oh CJ,Park S,Kim JY,Kim HJ,Doo Kim N,Choi YK,Do JY,Go Y,Ha CM,Choi JY,Huh S,Ho Jeoung N,Lee KU,Choi HS,Wang Y,Park KG,Harris RA,Lee IK

Published figure using SMAD1 polyclonal antibody (Product # 38-5400) in Western Blot

Thu Nov 12 00:00:00 EST 2015

Recurrent somatic mutations in ACVR1 in pediatric midline high-grade astrocytoma.

Nature genetics

Fontebasso AM,Papillon-Cavanagh S,Schwartzentruber J,Nikbakht H,Gerges N,Fiset PO,Bechet D,Faury D,De Jay N,Ramkissoon LA,Corcoran A,Jones DT,Sturm D,Johann P,Tomita T,Goldman S,Nagib M,Bendel A,Goumnerova L,Bowers DC,Leonard JR,Rubin JB,Alden T,Browd S,Geyer JR,Leary S,Jallo G,Cohen K,Gupta N,Prados MD,Carret AS,Ellezam B,Crevier L,Klekner A,Bognar L,Hauser P,Garami M,Myseros J,Dong Z,Siegel PM,Malkin H,Ligon AH,Albrecht S,Pfister SM,Ligon KL,Majewski J,Jabado N,Kieran MW

38-5400 was used in immunohistochemistry - paraffin section to identify therapeutic targets for pediatric midline high-grade astrocytomas.

Thu May 01 00:00:00 EDT 2014

Expression of GATA3 in MDA-MB-231 triple-negative breast cancer cells induces a growth inhibitory response to TGFß.

PloS one

Chu IM,Lai WC,Aprelikova O,El Touny LH,Kouros-Mehr H,Green JE

38-5400 was used in western blot to examine the relation of GATA3 and TGF beta in breast cancer cells.

Tue Oct 22 00:00:00 EDT 2013

Transforming growth factor β inhibits bone morphogenetic protein-induced transcription through novel phosphorylated Smad1/5-Smad3 complexes.

Molecular and cellular biology

Grönroos E,Kingston IJ,Ramachandran A,Randall RA,Vizán P,Hill CS

38-5400 was used in western blot to study the relationship between bone morphogenetic protein and TGF-β.

Sun Jul 01 00:00:00 EDT 2012

SNW1 is a critical regulator of spatial BMP activity, neural plate border formation, and neural crest specification in vertebrate embryos.

PLoS biology

Wu MY,Ramel MC,Howell M,Hill CS

Published figure using SMAD1 polyclonal antibody (Product # 38-5400) in Western Blot

Tue Feb 15 00:00:00 EST 2011

Distinct modes of inhibition by sclerostin on bone morphogenetic protein and Wnt signaling pathways.

The Journal of biological chemistry

Krause C,Korchynskyi O,de Rooij K,Weidauer SE,de Gorter DJ,van Bezooijen RL,Hatsell S,Economides AN,Mueller TD,Löwik CW,ten Dijke P

Fri Dec 31 00:00:00 EST 2010

Distinct modes of inhibition by sclerostin on bone morphogenetic protein and Wnt signaling pathways.

The Journal of biological chemistry

Krause C,Korchynskyi O,de Rooij K,Weidauer SE,de Gorter DJ,van Bezooijen RL,Hatsell S,Economides AN,Mueller TD,Löwik CW,ten Dijke P

Fri Dec 31 00:00:00 EST 2010

Growth/differentiation factor-15 inhibits differentiation into osteoclasts--a novel factor involved in control of osteoclast differentiation.

Differentiation; research in biological diversity

Vanhara P,Lincová E,Kozubík A,Jurdic P,Soucek K,Smarda J

38-5400 was used in western blot to discuss the role of GDF-15 in modulation of osteoclast differentiation

Sun Nov 01 00:00:00 EDT 2009

BMP7 and SHH regulate Pax2 in mouse retinal astrocytes by relieving TLX repression.

Developmental biology

Sehgal R,Sheibani N,Rhodes SJ,Belecky Adams TL

Sat Aug 15 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: BSP-1; Dwarfin-A; Dwf-A; DWFC; hSMAD1; JV4-1; MAD homolog 1; MAD, mothers against decapentaplegic homolog 1; Mad-related protein 1; MADH5; mMad1; Mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; Mothers-against-DPP-related 1; Mothers-against-DPP-related-1; OTTHUMP00000220456; SMAD; SMAD 1; SMAD family member 1; SMAD, mothers against DPP homolog 1; SMAD5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1

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Gene Aliases: AI528653; BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1; Mlp1; MusMLP; SMAD1

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UniProt ID: (Human) Q15797, (Mouse) P70340

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Entrez Gene ID: (Human) 4086, (Mouse) 17125

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding receptor signaling protein activity protein binding protein kinase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity identical protein binding protein homodimerization activity metal ion binding protein heterodimerization activity co-SMAD binding I-SMAD binding DNA binding DNA-binding transcription factor gene-specific transcriptional regulator

Process(es)

MAPK cascade ureteric bud development mesodermal cell fate commitment osteoblast fate commitment transcription from RNA polymerase II promoter inflammatory response signal transduction transforming growth factor beta receptor signaling pathway SMAD protein complex assembly gamete generation negative regulation of cell proliferation embryonic pattern specification positive regulation of gene expression BMP signaling pathway midbrain development hindbrain development primary miRNA processing homeostatic process positive regulation of osteoblast differentiation positive regulation of transcription from RNA polymerase II promoter cartilage development cardiac muscle cell proliferation bone development SMAD protein signal transduction positive regulation of cartilage development positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein phosphorylation response to drug positive regulation of cell differentiation cellular response to organic cyclic compound cellular response to BMP stimulus

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SMAD1 Polyclonal Antibody

SMAD1 Antibody (38-5400) in IHC (P)

Product Details

38-5400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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