Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
SMAD1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
SMAD1 Antibody (PA5-17122) in WB
Western blot was performed using anti-SMAD1 Polyclonal Antibody (Product # PA5-17122) and a 55 kDa band corresponding to SMAD1 was observed across the cell lines tested. Modified Whole cell extracts (30 µg lysate) (1% SDS) of T-47D (Lane 1), OVCAR-3 (Lane 2), HeLa (Lane 3) and HCT 116 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed w... View More
Product Details
PA5-17122
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues surrounding Ser190 of human Smad1
Conjugate
Form
Liquid
Concentration
83 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10980026
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BSP-1; Dwarfin-A; Dwf-A; DWFC; hSMAD1; JV4-1; MAD homolog 1; MAD, mothers against decapentaplegic homolog 1; Mad-related protein 1; MADH5; mMad1; Mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; Mothers-against-DPP-related 1; Mothers-against-DPP-related-1; OTTHUMP00000220456; SMAD; SMAD 1; SMAD family member 1; SMAD, mothers against DPP homolog 1; SMAD5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1
Gene Aliases: AI528653; BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1; Mlp1; MusMLP; SMAD1
UniProt ID: (Human) Q15797, (Mouse) P70340
Entrez Gene ID: (Human) 4086, (Mouse) 17125
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
receptor signaling protein activity
protein binding
protein kinase binding
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
identical protein binding
protein homodimerization activity
metal ion binding
protein heterodimerization activity
co-SMAD binding
I-SMAD binding
DNA binding
DNA-binding transcription factor
gene-specific transcriptional regulator
MAPK cascade
ureteric bud development
mesodermal cell fate commitment
osteoblast fate commitment
transcription from RNA polymerase II promoter
inflammatory response
signal transduction
transforming growth factor beta receptor signaling pathway
SMAD protein complex assembly
gamete generation
negative regulation of cell proliferation
embryonic pattern specification
positive regulation of gene expression
BMP signaling pathway
midbrain development
hindbrain development
primary miRNA processing
homeostatic process
positive regulation of osteoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
cartilage development
cardiac muscle cell proliferation
bone development
SMAD protein signal transduction
positive regulation of cartilage development
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein phosphorylation
response to drug
positive regulation of cell differentiation
cellular response to organic cyclic compound
cellular response to BMP stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support