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SMAD1 Antibody (MA5-41184) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of SMAD1 in MCF-7 cells (green) using SMAD1 Recombinant Monoclonal Antibody (Product # MA5-41184). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. The nuclear counter stain is DAPI (blue).
Product Details
MA5-41184
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SD2090
Immunogen
recombinant protein
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2898938
Target Information
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BSP-1; DWFC; hSMAD1; JV4-1; MAD homolog 1; MAD, mothers against decapentaplegic homolog 1; Mad-related protein 1; MADH5; Mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; Mothers-against-DPP-related-1; OTTHUMP00000220456; SMAD; SMAD 1; SMAD family member 1; SMAD, mothers against DPP homolog 1; SMAD5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1
Gene Aliases: BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1; SMAD1
UniProt ID: (Human) Q15797
Entrez Gene ID: (Human) 4086
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
receptor signaling protein activity
protein binding
protein kinase binding
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
identical protein binding
protein homodimerization activity
metal ion binding
protein heterodimerization activity
co-SMAD binding
I-SMAD binding
DNA-binding transcription factor
gene-specific transcriptional regulator
MAPK cascade
ureteric bud development
mesodermal cell fate commitment
osteoblast fate commitment
transcription from RNA polymerase II promoter
inflammatory response
signal transduction
transforming growth factor beta receptor signaling pathway
SMAD protein complex assembly
gamete generation
negative regulation of cell proliferation
embryonic pattern specification
positive regulation of gene expression
BMP signaling pathway
midbrain development
hindbrain development
primary miRNA processing
homeostatic process
positive regulation of osteoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
cartilage development
cardiac muscle cell proliferation
bone development
SMAD protein signal transduction
positive regulation of cartilage development
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
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