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SMAD2 Antibody (600-401-A59) in WB
Western blot using Rocklands affinity purified anti-Smad2 to detect over-expressed Smad2 in COS cells (arrow). Lane C shows mock infection of COS cells with lentiviral vector alone. Lane S2 shows detection of Smad2 in lysates of COS transfected with Smad2. Lane V contains lysates of MDA-MB231 cells treated with vehicle; the next lane contains lysates of MDA-MB231 cells treated with TGF beta. Low levels of staining in control lanes correspond to detection of endogenous Smad2. Pre-incubation of the antibody with immunizing peptide (data not ... View More
Product Details
600-401-A59
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad2 protein.
Conjugate
Form
Liquid
Concentration
0.55 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Directed against human Smad2 protein. A BLAST analysis was used to suggest cross-reactivity with Smad2 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known. Also, the antibody is Smad2 specific, and reactivity to other Smad proteins (specifically Smad1, Smad3, Smad4, and Smad7) is not detected in over-expressed cell lysates (Personal Communication, Kathleen Flanders, CCR-NCI, Bethesda, MD).
Target Information
SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hMAD-2; JV18-1; MAD; MAD homolog 2; Mad protein homolog; Mad-related protein 2; MGC22139; MGC34440; mMad2; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; mothers against DPP homolog 2; pSMAD2; Sma- and Mad-related protein 2; SMAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; Smad2Deltaexon3
Gene Aliases: 7120426M23Rik; hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2; mMad2; Smad-2; SMAD2
UniProt ID: (Human) Q15796, (Mouse) Q62432, (Rat) O70436
Entrez Gene ID: (Human) 4087, (Mouse) 17126, (Rat) 29357
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
transforming growth factor beta receptor binding
protein binding
transcription factor binding
phosphatase binding
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
ubiquitin protein ligase binding
activating transcription factor binding
type I transforming growth factor beta receptor binding
enhancer binding
protein homodimerization activity
SMAD binding
metal ion binding
protein heterodimerization activity
co-SMAD binding
I-SMAD binding
R-SMAD binding
DNA-binding transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
ureteric bud development
in utero embryonic development
endoderm formation
mesoderm formation
transcription from RNA polymerase II promoter
transforming growth factor beta receptor signaling pathway
common-partner SMAD protein phosphorylation
SMAD protein complex assembly
zygotic specification of dorsal/ventral axis
gastrulation
negative regulation of cell proliferation
response to glucose
post-embryonic development
anterior/posterior pattern specification
positive regulation of epithelial to mesenchymal transition
regulation of transforming growth factor beta receptor signaling pathway
signal transduction involved in regulation of gene expression
insulin secretion
lung development
negative regulation of transforming growth factor beta receptor signaling pathway
positive regulation of BMP signaling pathway
pancreas development
primary miRNA processing
activin receptor signaling pathway
somatic stem cell population maintenance
organ growth
intracellular signal transduction
nodal signaling pathway
cell fate commitment
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
paraxial mesoderm morphogenesis
embryonic foregut morphogenesis
embryonic cranial skeleton morphogenesis
regulation of binding
palate development
pericardium development
SMAD protein signal transduction
response to cholesterol
positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
pattern specification process
endoderm development
heart development
embryonic pattern specification
positive regulation of gene expression
negative regulation of gene expression
developmental growth
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