Invitrogen

SMAD2 Recombinant Rabbit Monoclonal Antibody (31H15L4)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

5 Published Figures

6 References

View all (67) SMAD2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

SMAD2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

SMAD2 Antibody (700048) in ICC/IF

Immunofluorescence analysis of SMAD2 was done on 70% confluent log phase TGF- beta treated HeLa cells (serum starved for 16 hours followed by treatment with 20 ng/mL TGF-beta for 1 hour). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with SMAD2 Recombinant Rabbit Monoclonal Antibody (Product # 700048) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alex... View More

SMAD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

SMAD2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. HeLa cells were transfected with SMAD2 siRNA and decrease in signal intensity was observed in Western blot application (Fig a) using Anti-SMAD2 Recombinant Rabbit Monoclonal Antibody (31H15L4) (Product # 700048). Densitometric analysis of this Western blot is shown in histogram (Fig b). {KD}

Product Details

700048

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/mL

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells

ELISA (ELISA)

1-5 µg/mL

ChIP assay (ChIP)

3 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

31H15L4

Immunogen

A peptide corresponding to amino acids 81-107 of Q15796.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532277

Product Specific Information

This antibody is predicted to react with bovine, carp, chicken, goldfish, mouse, opossum, orangutan, rat, Xenopus and zebrafish based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sulforaphane Inhibits the Expression of Long Noncoding RNA H19 and Its Target APOBEC3G and Thereby Pancreatic Cancer Progression.

Cancers

Luo Y,Yan B,Liu L,Yin L,Ji H,An X,Gladkich J,Qi Z,De La Torre C,Herr I

700048 was used in Western Blotting to identify APOBEC3G as H19 target, and both are inhibited by sulforaphane in prevention of PDAC progression.

Tue Feb 16 00:00:00 EST 2021

Sulforaphane Inhibits the Expression of Long Noncoding RNA H19 and Its Target APOBEC3G and Thereby Pancreatic Cancer Progression.

Cancers

Luo Y,Yan B,Liu L,Yin L,Ji H,An X,Gladkich J,Qi Z,De La Torre C,Herr I

700048 was used in Western Blotting to identify APOBEC3G as H19 target, and both are inhibited by sulforaphane in prevention of PDAC progression.

Tue Feb 16 00:00:00 EST 2021

Evaluation of the inhibitory effect of tacrolimus combined with mycophenolate mofetil on mesangial cell proliferation based on the cell cycle.

International journal of molecular medicine

Gao Y,Yang H,Wang Y,Tian J,Li R,Zhou X

700048 was used in Western Blotting to validate and reinforce the potential use of the combination of tacrolimus (TAC) and mycophenolate mofetil (MMF) for the treatment of mesangial proliferative diseases.

Thu Oct 01 00:00:00 EDT 2020

Evaluation of the inhibitory effect of tacrolimus combined with mycophenolate mofetil on mesangial cell proliferation based on the cell cycle.

International journal of molecular medicine

Gao Y,Yang H,Wang Y,Tian J,Li R,Zhou X

Thu Oct 01 00:00:00 EDT 2020

PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer.

Cell reports

Lin LL,Kost ER,Lin CL,Valente P,Wang CM,Kolonin MG,Daquinag AC,Tan X,Lucio N,Hung CN,Wang CP,Kirma NB,Huang TH

700048 was used in Mass cytometry to investigate the role of plasminogen activator inhibitor-1 signaling in rendering ineffective intercellular communication for the development of adiposity-associated endometrial cancer.

Tue Oct 13 00:00:00 EDT 2020

Long-Range Signaling Activation and Local Inhibition Separate the Mesoderm and Endoderm Lineages.

Developmental cell

van Boxtel AL,Economou AD,Heliot C,Hill CS

Published figure using SMAD2 monoclonal antibody (Product # 700048) in ChIP assay

Mon Jan 22 00:00:00 EST 2018

Hypoxia-induced endothelial-mesenchymal transition is associated with RASAL1 promoter hypermethylation in human coronary endothelial cells.

FEBS letters

Xu X,Tan X,Hulshoff MS,Wilhelmi T,Zeisberg M,Zeisberg EM

700048 was used in western blot to characterize the association with RASAL1 promoter hypermethylation in human coronary endothelial cells and hypoxia-induced endothelial-mesenchymal transition

Fri Apr 01 00:00:00 EDT 2016

FGFR2IIIb-MAPK Activity Is Required for Epithelial Cell Fate Decision in the Lower Müllerian Duct.

Molecular endocrinology (Baltimore, Md.)

Terakawa J,Rocchi A,Serna VA,Bottinger EP,Graff JM,Kurita T

700048 was used in immunohistochemistry - paraffin section to learn the requirement for epithelial cell fate decision in the lower mullerian duct by FGFR2IIIb-MAPK activity

Fri Jul 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: hMAD-2; JV18-1; MAD; MAD homolog 2; Mad protein homolog; Mad-related protein 2; MGC22139; MGC34440; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; pSMAD2; Sma- and Mad-related protein 2; SMAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; Smad2Deltaexon3

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Gene Aliases: hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2; SMAD2

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UniProt ID: (Human) Q15796

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Entrez Gene ID: (Human) 4087

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding transforming growth factor beta receptor binding protein binding transcription factor binding phosphatase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity ubiquitin protein ligase binding activating transcription factor binding type I transforming growth factor beta receptor binding enhancer binding protein homodimerization activity SMAD binding metal ion binding protein heterodimerization activity co-SMAD binding I-SMAD binding R-SMAD binding DNA-binding transcription factor gene-specific transcriptional regulator

Process(es)

negative regulation of transcription from RNA polymerase II promoter ureteric bud development in utero embryonic development endoderm formation mesoderm formation transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway common-partner SMAD protein phosphorylation SMAD protein complex assembly zygotic specification of dorsal/ventral axis gastrulation negative regulation of cell proliferation response to glucose post-embryonic development anterior/posterior pattern specification positive regulation of epithelial to mesenchymal transition regulation of transforming growth factor beta receptor signaling pathway signal transduction involved in regulation of gene expression insulin secretion lung development negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of BMP signaling pathway pancreas development primary miRNA processing activin receptor signaling pathway somatic stem cell population maintenance organ growth intracellular signal transduction nodal signaling pathway cell fate commitment negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter paraxial mesoderm morphogenesis embryonic foregut morphogenesis embryonic cranial skeleton morphogenesis regulation of binding palate development pericardium development SMAD protein signal transduction response to cholesterol positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SMAD2 Recombinant Rabbit Monoclonal Antibody (31H15L4)

SMAD2 Antibody (700048) in ICC/IF

Product Details

700048

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

ChIP assay (ChIP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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