Proteintech

SMAD3 Monoclonal Antibody (1F10B2)

SMAD3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SMAD3 Antibody (66516-1-IG) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using (Product # 66516-1-IG) (SMAD3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Product Image Gallery

Product Details

66516-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 40 publications 40 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:400

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1F10B2

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Target Information

SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Efficacy of Danggui Buxue decoction on diabetic nephropathy-induced renal fibrosis in rats and possible mechanism.

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan

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Thu Jun 01 00:00:00 EDT 2023

CCL17 drives fibroblast activation in the progression of pulmonary fibrosis by enhancing the TGF-β/Smad signaling.

Biochemical pharmacology

Wang QR,Liu SS,Min JL,Yin M,Zhang Y,Zhang Y,Tang XN,Li X,Liu SS

Sat Apr 01 00:00:00 EDT 2023

The miR-103a-3p/TGFBR3 axis regulates TGF-β-induced orbital fibroblast activation and fibrosis in thyroid-eye disease.

Molecular and cellular endocrinology

Xie B,Xiong W,Zhang F,Wang N,Luo Y,Chen Y,Cao J,Chen Z,Ma C,Chen H

Sun Jan 01 00:00:00 EST 2023

Elevated Angiotensin-II Levels Contribute to the Pathogenesis of Open-Angle Glaucoma Via Inducing the Expression of Fibrosis-Related Genes in Trabecular Meshwork Cells Through a ROS/NOX4/SMAD3 Axis.

Cell transplantation

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Mon Apr 03 00:00:00 EDT 2023

Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion.

Cellular and molecular life sciences : CMLS

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Tue Jun 20 00:00:00 EDT 2023

Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion.

Cellular and molecular life sciences : CMLS

Ye S,Huang H,Xiao Y,Han X,Shi F,Luo W,Chen J,Ye Y,Zhao X,Huang W,Wang Y,Lai D,Liang G,Fu G

Tue Jun 20 00:00:00 EDT 2023

Squalene epoxidase promotes hepatocellular carcinoma development by activating STRAP transcription and TGF-β/SMAD signalling.

British journal of pharmacology

Zhang Z,Wu W,Jiao H,Chen Y,Ji X,Cao J,Yin F,Yin W

Thu Jun 01 00:00:00 EDT 2023

Knockdown of ABCB7 inhibits esophageal cancer progression by inhibiting the TGF-β/Smad signaling.

Archives of biochemistry and biophysics

Feng S,Jia J,Lv G,Wang Y

Sat Jul 01 00:00:00 EDT 2023

TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment.

iScience

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Fri Apr 21 00:00:00 EDT 2023

Dexamethasone Induces Senescence-Associated Changes in Trabecular Meshwork Cells by Increasing ROS Levels Via the TGFβ/Smad3-NOX4 Axis.

Cell transplantation

Li H,Ren J,Cui H,Wang D,Zhao R,Liu X,Tian S,Wang J,Zhang J,Li P,Thorne RF,Duan S

Mon Jun 05 00:00:00 EDT 2023

BSHXF-medicated serum combined with ADSCs regulates the TGF-β1/Smad pathway to repair oxidatively damaged NPCs and its component analysis.

Journal of ethnopharmacology

Duan J,Li Z,Liu E,Long H,Chen L,Yang S

Wed Nov 15 00:00:00 EST 2023

Thymoquinone affects the gemcitabine sensitivity of pancreatic cancer by regulating collagen via hypoxia inducible factor-1α.

Frontiers in pharmacology

Zhao Z,Liu L,Chen H,Li S,Guo Y,Hou X,Yang J

Mon Jun 19 00:00:00 EDT 2023

Two distinct males absent on the first (MOF)-containing histone acetyltransferases are involved in the epithelial-mesenchymal transition in different ways in human cells.

Cellular and molecular life sciences : CMLS

Wei T,Liu H,Zhu H,Chen W,Wu T,Bai Y,Zhang X,Miao Y,Wang F,Cai Y,Jin J

Wed Apr 13 00:00:00 EDT 2022

Synthesis and structure-activity relationship study of a potent MHO7 analogue as potential anti-triple negative breast cancer agent.

European journal of medicinal chemistry

Liang JJ,Yu WL,Yang L,Qin KM,Yin YP,Li D,Ni YH,Yan JJ,Zhong YX,Deng ZX,Hong K

Sun Jun 05 00:00:00 EDT 2022

Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Wu L,Yang FR,Xing ML,Lu SF,Chen HL,Yang QW,Zhang YT,Lu Y,Huang Y

Wed Jun 01 00:00:00 EDT 2022

Hyperglycemia induces miR-26-5p down-regulation to overexpress PFKFB3 and accelerate epithelial-mesenchymal transition in gastric cancer.

Bioengineered

He X,Cheng X,Ding J,Xiong M,Chen B,Cao G

Tue Feb 01 00:00:00 EST 2022

Botox-A improve the thyroid-associated ophthalmopathy (TAO) orbital fibroblast activation through inhibiting the TGF-β/Smad signaling.

Experimental eye research

Qi X,Luo B,Deng M,Cao J,Hou S,Xie Z,Tong B,Xiong W

Fri Apr 01 00:00:00 EDT 2022

JPHYD Inhibits miR-21-5p/Smad7-Mediated Epithelial-Mesenchymal Transition of Hepatocellular Carcinoma Cells.

Journal of oncology

Liu LH,Fang CK,Ge FC,Wang JN,Zhang XB,Luo R,Zhang Y,Feng KL,Qiu ZW,Zhong C

Sat Jul 16 00:00:00 EDT 2022

Genetic or siRNA inhibition of MBD2 attenuates the UUO- and I/R-induced renal fibrosis via downregulation of EGR1.

Molecular therapy. Nucleic acids

Ai K,Li X,Zhang P,Pan J,Li H,He Z,Zhang H,Yi L,Kang Y,Wang Y,Chen J,Li Y,Xiang X,Chai X,Zhang D

Tue Jun 14 00:00:00 EDT 2022

Loureirin A Exerts Antikeloid Activity by Antagonizing the TGF-β1/Smad Signalling Pathway.

Evidence-based complementary and alternative medicine : eCAM

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Thu Dec 21 00:00:00 EST 2023

Celastrol attenuates renal injury in 5/6 nephrectomized rats via inhibiting epithelial-mesenchymal transition and transforming growth factor-β1/Smad3 pathway.

Experimental biology and medicine (Maywood, N.J.)

Tang YW,Yang RC,Wan F,Tang XL,Zhang HQ,Lin Y

Tue Nov 01 00:00:00 EDT 2022

IL-22 inhibits bleomycin-induced pulmonary fibrosis in association with inhibition of IL-17A in mice.

Arthritis research & therapy

Qu Z,Dou W,Zhang K,Duan L,Zhou D,Yin S

Sat Dec 24 00:00:00 EST 2022

Sestrin2 remedies podocyte injury via orchestrating TSP-1/TGF-β1/Smad3 axis in diabetic kidney disease.

Cell death & disease

Song S,Shi C,Bian Y,Yang Z,Mu L,Wu H,Duan H,Shi Y

Sat Jul 30 00:00:00 EDT 2022

Metformin Ameliorates Chronic Colitis-Related Intestinal Fibrosis via Inhibiting TGF-β1/Smad3 Signaling.

Frontiers in pharmacology

Wang Y,Wang Z,Yang H,Chen S,Zheng D,Liu X,Jiang Q,Chen Y

Sat Jul 16 00:00:00 EDT 2022

Anti-fibrotic effect of extracellular vesicles derived from tea leaves in hepatic stellate cells and liver fibrosis mice.

Frontiers in nutrition

Gong Q,Zeng Z,Jiang T,Bai X,Pu C,Hao Y,Guo Y

Tue Oct 25 00:00:00 EDT 2022

Hepatoprotective Efficacy and Interventional Mechanism of Qijia Rougan Decoction in Liver Fibrosis.

Frontiers in pharmacology

Chen XF,Wang Y,Ji S,Sun X,Feng Q,Yu H,Liu C

Tue Jul 19 00:00:00 EDT 2022

The Traditional Chinese Medicine Formula FTZ Protects against Cardiac Fibrosis by Suppressing the TGFβ1-Smad2/3 Pathway.

Evidence-based complementary and alternative medicine : eCAM

Zhang Y,Wang D,Wu K,Shao X,Diao H,Wang Z,Sun M,Huang X,Li Y,Tang X,Yan M,Guo J

Sat Jul 16 00:00:00 EDT 2022

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Hu H,Mao G,Zheng J,Guo F

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Smad7 Is Highly Expressed in Human Degenerative Discs and Participates in IL-1β-Induced Apoptosis of Rat AF Cells via the Mitochondria Pathway.

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Li B,Lu ZY,Jiang SD,Jiang LS,Zheng XF

Fri Jul 08 00:00:00 EDT 2022

Iodine-125 Seeds Inhibit Carcinogenesis of Hepatocellular Carcinoma Cells by Suppressing Epithelial-Mesenchymal Transition via TGF-β1/Smad Signaling.

Disease markers

Yang C,Xiao Y,Du Y,Xiong J,Deng L,Liang Q,Yuan J,He C,He F,Huang X

Wed May 18 00:00:00 EDT 2022

Jianpi Huayu decoction inhibits the epithelial-mesenchymal transition of hepatocellular carcinoma cells by suppressing exosomal miR-23a-3p/Smad signaling.

Journal of ethnopharmacology

Xie CF,Feng KL,Wang JN,Luo R,Fang CK,Zhang Y,Shen CP,Zhong C

Wed Aug 10 00:00:00 EDT 2022

Jianpi Huayu decoction inhibits the epithelial-mesenchymal transition of hepatocellular carcinoma cells by suppressing exosomal miR-23a-3p/Smad signaling.

Journal of ethnopharmacology

Xie CF,Feng KL,Wang JN,Luo R,Fang CK,Zhang Y,Shen CP,Zhong C

Wed Aug 10 00:00:00 EDT 2022

Jianpi Huayu decoction inhibits the epithelial-mesenchymal transition of hepatocellular carcinoma cells by suppressing exosomal miR-23a-3p/Smad signaling.

Journal of ethnopharmacology

Xie CF,Feng KL,Wang JN,Luo R,Fang CK,Zhang Y,Shen CP,Zhong C

Wed Aug 10 00:00:00 EDT 2022

Jianpi Huayu decoction inhibits the epithelial-mesenchymal transition of hepatocellular carcinoma cells by suppressing exosomal miR-23a-3p/Smad signaling.

Journal of ethnopharmacology

Xie CF,Feng KL,Wang JN,Luo R,Fang CK,Zhang Y,Shen CP,Zhong C

Wed Aug 10 00:00:00 EDT 2022

PPARγ Mediates the Anti-Epithelial-Mesenchymal Transition Effects of FGF1^ΔHBS in Chronic Kidney Diseases via Inhibition of TGF-β1/SMAD3 Signaling.

Frontiers in pharmacology

Wang D,Zhao T,Zhao Y,Yin Y,Huang Y,Cheng Z,Wang B,Liu S,Pan M,Sun D,Wang Z,Zhu G

Tue Jun 22 00:00:00 EDT 2021

Presegetane diterpenoids from Euphorbia sieboldiana as a new type of anti-liver fibrosis agents that inhibit TGF-β/Smad signaling pathway.

Bioorganic chemistry

Li S,Gan L,Tian YJ,Tian Y,Fan RZ,Huang D,Yuan FY,Zhang X,Lin Y,Zhu QF,Tang GH,Yan XL,Yin S

Wed Sep 01 00:00:00 EDT 2021

The Combination of Schisandrol B and Wedelolactone Synergistically Reverses Hepatic Fibrosis Via Modulating Multiple Signaling Pathways in Mice.

Frontiers in pharmacology

Ai Y,Shi W,Zuo X,Sun X,Chen Y,Wang Z,Li R,Song X,Dai W,Mu W,Ding K,Li Z,Li Q,Xiao X,Zhan X,Bai Z

Mon Oct 25 00:00:00 EDT 2021

Knockdown of ALDH1A3 reduces breast cancer stem cell marker CD44 via the miR-7-TGFBR2-Smad3-CD44 regulatory axis.

Experimental and therapeutic medicine

Pan M,Li M,Guo M,Zhou H,Xu H,Zhao F,Mei F,Xue R,Dou J

Fri Oct 01 00:00:00 EDT 2021

Traditional Chinese medicine Yiqi Huoxue recipe attenuates hepatic fibrosis via YAP/TAZ signaling.

Histology and histopathology

Zhao W,Zhang X,Hou M,Zhang Y,Tang Y,Li L,Dong S,Liu L,Zhao D,Li W,Nan Y

Wed Sep 01 00:00:00 EDT 2021

Traditional Chinese medicine Yiqi Huoxue recipe attenuates hepatic fibrosis via YAP/TAZ signaling.

Histology and histopathology

Zhao W,Zhang X,Hou M,Zhang Y,Tang Y,Li L,Dong S,Liu L,Zhao D,Li W,Nan Y

Wed Sep 01 00:00:00 EDT 2021

Amygdalin inhibits TGFβ1-induced activation of hepatic stellate cells (HSCs) in vitro and CCl₄-induced hepatic fibrosis in rats in vivo.

International immunopharmacology

Wang R,Zhang D,Tang D,Sun K,Peng J,Zhu W,Yin S,Wu Y

Fri Jan 01 00:00:00 EST 2021

Study on the potential nephrotoxicity and mutagenicity of aristolochic acid IVa and its mechanism.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Xian Z,Tian J,Zhang Y,Meng J,Zhao Y,Li C,Yi Y,Han J,Liu S,Wang L,Pan C,Wang D,Wang F,Liang A

Fri Oct 01 00:00:00 EDT 2021

Anti-Fibrosis Effects of Magnesium Lithospermate B in Experimental Pulmonary Fibrosis: By Inhibiting TGF-βRI/Smad Signaling.

Molecules (Basel, Switzerland)

Luo X,Deng Q,Xue Y,Zhang T,Wu Z,Peng H,Xuan L,Pan G

Fri Mar 19 00:00:00 EDT 2021

miR-96-5p regulated TGF-β/SMAD signaling pathway and suppressed endometrial cell viability and migration via targeting TGFBR1.

Cell cycle (Georgetown, Tex.)

Chen S,Luo Y,Cui L,Yang Q

Wed Jul 01 00:00:00 EDT 2020

Quercetin ameliorates podocyte injury via inhibition of oxidative stress and the TGF-β1/Smad pathway in DN rats.

RSC advances

Gao F,He X,Liang S,Liu S,Liu H,He Q,Chen L,Jiang H,Zhang Y

Mon Oct 15 00:00:00 EDT 2018

Thymoquinone protects against cardiac damage from doxorubicin-induced heart failure in Sprague-Dawley rats.

RSC advances

Pei Z,Hu J,Bai Q,Liu B,Cheng D,Liu H,Na R,Yu Q

Tue Apr 17 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: DKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; JV15-2; MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; MAD-3; mad3; MGC60396; mMad3; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD; SMAD 3; SMAD family member 3; SMAD, mothers against DPP homolog 3

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Gene Aliases: AU022421; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; mad3; MADH3; Smad 3; SMAD3

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UniProt ID: (Human) P84022, (Mouse) Q8BUN5, (Rat) P84025

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Entrez Gene ID: (Human) 4088, (Mouse) 17127, (Rat) 25631

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter sequence-specific core promoter proximal region sequence-specific DNA binding transcription factor activity, protein binding RNA polymerase II activating transcription factor binding transcription factor activity, sequence-specific DNA binding transforming growth factor beta receptor binding protein binding collagen binding transcription factor binding zinc ion binding protein kinase binding phosphatase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity chromatin DNA binding ubiquitin protein ligase binding enhancer binding identical protein binding protein homodimerization activity ubiquitin binding bHLH transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding protein heterodimerization activity co-SMAD binding R-SMAD binding DNA binding chromatin binding double-stranded DNA binding beta-catenin binding enzyme binding SMAD binding metal ion binding DNA-binding transcription factor gene-specific transcriptional regulator

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SMAD3 Monoclonal Antibody (1F10B2)

SMAD3 Antibody (66516-1-IG) in IHC (P)

Product Details

66516-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Target Information

Bioinformatics

Performance Guarantee

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