Invitrogen

SMAD3 Monoclonal Antibody (5G11)

SMAD3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

SMAD3 Antibody (MA5-15663) in ICC/IF

Immunofluorescence analysis of NIH/3T3 cells using SMAD3 monoclonal antibody (Product # MA5-15663) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

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Product Details

MA5-15663

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200-1:1,000

View 1 publication 1 publication

ELISA (ELISA)

1:10,000

in situ PLA (PLA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5G11

Immunogen

Purified recombinant fragment of human SMAD3 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10983274

Product Specific Information

MA5-15663 targets SMAD3 in indirect ELISA, ICC, IF and WB applications and shows reactivity with Human and mouse samples.

The MA5-15663 immunogen is purified recombinant fragment of human SMAD3 expressed in E. Coli.
.

MA5-15663 detects SMAD3 which has a predicted molecular weight of approximately 48kDa.

Target Information

SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Core transcription regulatory circuitry orchestrates corneal epithelial homeostasis.

Nature communications

Li M,Huang H,Li L,He C,Zhu L,Guo H,Wang L,Liu J,Wu S,Liu J,Xu T,Mao Z,Cao N,Zhang K,Lan F,Ding J,Yuan J,Liu Y,Ouyang H

MA5-15663 was used in Western Blotting to highlight CRC TF cooperativity for establishment of stem cell identity and lineage commitment, and provides comprehensive regulatory principles for human stratified epithelial homeostasis and pathogenesis.

Mon Jan 18 00:00:00 EST 2021

Copy number variation and expression of exportin-4 associates with severity of fibrosis in metabolic associated fatty liver disease.

EBioMedicine

Metwally M,Bayoumi A,Khan A,Adams LA,Aller R,García-Monzón C,Arias-Loste MT,Bugianesi E,Miele L,Anna A,Latchoumanin O,Han S,Alenizi S,Sharkawy RE,Elattar A,Gallego-Durán R,Fischer J,Berg T,Liddle C,Romero-Gomez M,George J,Eslam M

MA5-15663 was used in Proximity Ligation Assay to investigate if duplication in the exportin 4 (XPO4) copy number variations is associated with the severity of liver fibrosis.

Sun Aug 01 00:00:00 EDT 2021

SOX4 can redirect TGF-β-mediated SMAD3-transcriptional output in a context-dependent manner to promote tumorigenesis.

Nucleic acids research

Vervoort SJ,Lourenço AR,Tufegdzic Vidakovic A,Mocholi E,Sandoval JL,Rueda OM,Frederiks C,Pals C,Peeters JGC,Caldas C,Bruna A,Coffer PJ

Published figure using SMAD3 monoclonal antibody (Product # MA5-15663) in Immunofluorescence

Fri Oct 12 00:00:00 EDT 2018

SOX4 can redirect TGF-β-mediated SMAD3-transcriptional output in a context-dependent manner to promote tumorigenesis.

Nucleic acids research

Vervoort SJ,Lourenço AR,Tufegdzic Vidakovic A,Mocholi E,Sandoval JL,Rueda OM,Frederiks C,Pals C,Peeters JGC,Caldas C,Bruna A,Coffer PJ

Published figure using SMAD3 monoclonal antibody (Product # MA5-15663) in Immunofluorescence

Fri Oct 12 00:00:00 EDT 2018

RIPK1-RIPK3-MLKL-dependent necrosis promotes the aging of mouse male reproductive system.

eLife

Li D,Meng L,Xu T,Su Y,Liu X,Zhang Z,Wang X

MA5-15663 was used in Immunohistochemistry to report that male reproductive organs of both Ripk3- and Mlkl-knockout mice retain ''youthful'' morphology and function into advanced age, while those of age-matched wild-type mice deteriorate.

Tue Aug 15 00:00:00 EDT 2017

Soluble MMP-14 produced by bone marrow-derived stromal cells sheds epithelial endoglin modulating the migratory properties of human breast cancer cells.

Carcinogenesis

Tobar N,Avalos MC,Méndez N,Smith PC,Bernabeu C,Quintanilla M,Martínez J

MA5-15663 was used in western blot to study the modulation of breast cancer cell migratory potential by endoglin shed from epithelial cells by soluble MMP14 from bone marrow-derived stromal cells

Fri Aug 01 00:00:00 EDT 2014

c-Jun N terminal kinase modulates NOX-4 derived ROS production and myofibroblasts differentiation in human breast stromal cells.

BMC cancer

Tobar N,Toyos M,Urra C,Méndez N,Arancibia R,Smith PC,Martínez J

MA5-15663 was used in western blot to examine the role of c-Jun N terminal kinase in myofibroblasts differentiation in human breast stromal cells

Sat Aug 30 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: DKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; JV15-2; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; MAD-3; mad3; MGC60396; mMad3; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD; SMAD 3; SMAD family member 3; SMAD, mothers against DPP homolog 3

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Gene Aliases: AU022421; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3; SMAD3

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UniProt ID: (Human) P84022, (Mouse) Q8BUN5

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Entrez Gene ID: (Human) 4088, (Mouse) 17127

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter sequence-specific core promoter proximal region sequence-specific DNA binding transcription factor activity, protein binding RNA polymerase II activating transcription factor binding transcription factor activity, sequence-specific DNA binding transforming growth factor beta receptor binding protein binding collagen binding transcription factor binding zinc ion binding protein kinase binding phosphatase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity chromatin DNA binding ubiquitin protein ligase binding enhancer binding identical protein binding protein homodimerization activity ubiquitin binding bHLH transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding protein heterodimerization activity co-SMAD binding R-SMAD binding DNA binding chromatin binding double-stranded DNA binding beta-catenin binding enzyme binding SMAD binding metal ion binding DNA-binding transcription factor gene-specific transcriptional regulator

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SMAD3 Monoclonal Antibody (5G11)

SMAD3 Antibody (MA5-15663) in ICC/IF

Product Details

MA5-15663

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

in situ PLA (PLA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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