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Bethyl Laboratories
SMAD3 Recombinant Rabbit Monoclonal Antibody (BLR144J)
FIGURE: 1 / 2
SMAD3 Antibody (A700-144) in WB
Detection of human SMAD3 by western blot. Samples: Whole cell lysate (50 µg) from PANC-1, HeLa, A-549, Jurkat, HEK293T, K-562, U2OS, and HT-29 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMAD3 recombinant monoclonal antibody [BLR144J] (A700-144 lot 1) used at 1:1,000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody (A304-523A).
Product Details
A700-144
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR144J
Conjugate
Form
Liquid
Concentration
400 µg/mL
Purification
purified
Storage buffer
BBS, pH 8.2, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; JV15-2; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; MAD-3; mad3; MGC60396; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD; SMAD 3; SMAD family member 3; SMAD, mothers against DPP homolog 3
Gene Aliases: HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3; SMAD3
UniProt ID: (Human) P84022
Entrez Gene ID: (Human) 4088
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter sequence-specific
core promoter proximal region sequence-specific DNA binding
transcription factor activity, protein binding
RNA polymerase II activating transcription factor binding
transcription factor activity, sequence-specific DNA binding
transforming growth factor beta receptor binding
protein binding
collagen binding
transcription factor binding
zinc ion binding
protein kinase binding
phosphatase binding
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
chromatin DNA binding
ubiquitin protein ligase binding
enhancer binding
identical protein binding
protein homodimerization activity
ubiquitin binding
bHLH transcription factor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
protein heterodimerization activity
co-SMAD binding
R-SMAD binding
DNA-binding transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
ureteric bud development
response to hypoxia
in utero embryonic development
mesoderm formation
somitogenesis
liver development
heart looping
osteoblast development
immune system development
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transport
activation of cysteine-type endopeptidase activity involved in apoptotic process
immune response
cell cycle arrest
transforming growth factor beta receptor signaling pathway
SMAD protein complex assembly
endoderm development
embryonic pattern specification
positive regulation of gene expression
positive regulation of alkaline phosphatase activity
positive regulation of epithelial to mesenchymal transition
regulation of striated muscle tissue development
regulation of transforming growth factor beta receptor signaling pathway
evasion or tolerance of host defenses by virus
signal transduction involved in regulation of gene expression
negative regulation of cell growth
positive regulation of cell migration
positive regulation of bone mineralization
negative regulation of transforming growth factor beta receptor signaling pathway
thyroid gland development
primary miRNA processing
positive regulation of chondrocyte differentiation
positive regulation of interleukin-1 beta production
regulation of transforming growth factor beta2 production
positive regulation of transforming growth factor beta3 production
activin receptor signaling pathway
negative regulation of osteoblast proliferation
nodal signaling pathway
wound healing
T cell activation
positive regulation of transcription factor import into nucleus
negative regulation of apoptotic process
cell-cell junction organization
negative regulation of fat cell differentiation
negative regulation of osteoblast differentiation
positive regulation of transcription, DNA-templated
negative regulation of mitotic cell cycle
positive regulation of transcription from RNA polymerase II promoter
paraxial mesoderm morphogenesis
developmental growth
embryonic foregut morphogenesis
embryonic cranial skeleton morphogenesis
regulation of epithelial cell proliferation
negative regulation of inflammatory response
regulation of immune response
positive regulation of positive chemotaxis
regulation of binding
positive regulation of stress fiber assembly
positive regulation of focal adhesion assembly
pericardium development
transdifferentiation
SMAD protein signal transduction
negative regulation of wound healing
lens fiber cell differentiation
extrinsic apoptotic signaling pathway
activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
positive regulation of extracellular matrix assembly
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