Proteintech

SMAD4 Polyclonal Antibody

SMAD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 20

SMAD4 Antibody (10231-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 10231-1-AP ( SMAD4 Antibody) at dilution of 1:50 (under 40x lens).

SMAD4 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10231-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 55 publications 55 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 4 publications 4 publications

ChIP assay (ChIP)

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SMAD4 Fusion Protein Ag0299 (13-227 aa encoded by BC002379)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against an internal region of human SMAD4.

Immunogen sequence: NDACLSIVH SLMCHRQGGE SETFAKRAIE SLVKKLKEKK DELDSLITAI TTNGAHPSKC VTIQRTLDGR LQVAGRKGFP HVIYARLWRW PDLHKNELKH VKYCQYAFDL KCDSVCVNPY HYERVVSPGI DLSGLTLQSN APSSMMVKDE YVHDFEGQPS LSTEGHSIQT IQHPPSNRAS TETYSTPALL APSESNATST ANFPNIPVAS TSQPAS (13-227 aa encoded by BC002379)

Target Information

Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-related protein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Possible mechanisms associated with immune escape and apoptosis on anti-hepatocellular carcinoma effect of Mu Ji Fang granules.

World journal of gastrointestinal oncology

Zhang YB,Bao YR,Wang S,Li TJ,Tai H,Leng JP,Yang XX,Wang BC,Meng XS

Wed Mar 15 00:00:00 EDT 2023

Effect of matrine in MAC-T cells and their transcriptome analysis: A basic study.

PloS one

Zhang Z,Yang Y,Yan L,Wan X,Sun K,Gou H,Ding J,Peng J,Liu G,Wang C

Tue Jan 31 00:00:00 EST 2023

Benzylisoquinoline alkaloids inhibit lung fibroblast activation mainly via inhibiting TGF-β1/Smads and ERK1/2 pathway proteins.

Heliyon

Ren HL,Zhang JH,Xiao JH

Thu Jun 01 00:00:00 EDT 2023

BMP4/SMAD8 signaling pathway regulated granular cell proliferation to promote follicle development in Wanxi white goose.

Poultry science

Wei C,Chen X,Peng J,Yu S,Chang P,Jin K,Geng Z

Sun Jan 01 00:00:00 EST 2023

Qianjin Wenwu decoction suppresses renal interstitial fibrosis by enhancing the degradation of extracellular matrix in mice with unilateral ureteral obstruction.

Chinese journal of natural medicines

Jin C,Wu X,You Y,Wang Y,Wu J,Zuo A,Zheng Y,Guo J

Sat Apr 01 00:00:00 EDT 2023

USP25 inhibits renal fibrosis by regulating TGFβ-SMAD signaling pathway in Ang II-induced hypertensive mice.

Biochimica et biophysica acta. Molecular basis of disease

Zhao Y,Chen X,Lin Y,Li Z,Su X,Fan S,Chen Y,Wang X,Liang G

Tue Aug 01 00:00:00 EDT 2023

USP25 inhibits renal fibrosis by regulating TGFβ-SMAD signaling pathway in Ang II-induced hypertensive mice.

Biochimica et biophysica acta. Molecular basis of disease

Zhao Y,Chen X,Lin Y,Li Z,Su X,Fan S,Chen Y,Wang X,Liang G

Tue Aug 01 00:00:00 EDT 2023

USP25 inhibits renal fibrosis by regulating TGFβ-SMAD signaling pathway in Ang II-induced hypertensive mice.

Biochimica et biophysica acta. Molecular basis of disease

Zhao Y,Chen X,Lin Y,Li Z,Su X,Fan S,Chen Y,Wang X,Liang G

Tue Aug 01 00:00:00 EDT 2023

Linc-ROR Promotes EMT by Targeting miR-204-5p/SMAD4 in Endometriosis.

Reproductive sciences (Thousand Oaks, Calif.)

Yi M,Wang S,Zhang X,Jiang L,Xia X,Zhang T,Fang X

Fri Sep 01 00:00:00 EDT 2023

Linc-ROR Promotes EMT by Targeting miR-204-5p/SMAD4 in Endometriosis.

Reproductive sciences (Thousand Oaks, Calif.)

Yi M,Wang S,Zhang X,Jiang L,Xia X,Zhang T,Fang X

Fri Sep 01 00:00:00 EDT 2023

Linc-ROR Promotes EMT by Targeting miR-204-5p/SMAD4 in Endometriosis.

Reproductive sciences (Thousand Oaks, Calif.)

Yi M,Wang S,Zhang X,Jiang L,Xia X,Zhang T,Fang X

Fri Sep 01 00:00:00 EDT 2023

Linc-ROR Promotes EMT by Targeting miR-204-5p/SMAD4 in Endometriosis.

Reproductive sciences (Thousand Oaks, Calif.)

Yi M,Wang S,Zhang X,Jiang L,Xia X,Zhang T,Fang X

Fri Sep 01 00:00:00 EDT 2023

Irisin Ameliorates Renal Tubulointerstitial Fibrosis by Regulating the Smad4/β-Catenin Pathway in Diabetic Mice.

Diabetes, metabolic syndrome and obesity : targets and therapy

Yang Z,Wei J,Wang Y,Du Y,Song S,Li J,Su Z,Shi Y,Wu H

Sun Jun 11 00:00:00 EDT 2023

Irisin Ameliorates Renal Tubulointerstitial Fibrosis by Regulating the Smad4/β-Catenin Pathway in Diabetic Mice.

Diabetes, metabolic syndrome and obesity : targets and therapy

Yang Z,Wei J,Wang Y,Du Y,Song S,Li J,Su Z,Shi Y,Wu H

Sun Jun 11 00:00:00 EDT 2023

Irisin Ameliorates Renal Tubulointerstitial Fibrosis by Regulating the Smad4/β-Catenin Pathway in Diabetic Mice.

Diabetes, metabolic syndrome and obesity : targets and therapy

Yang Z,Wei J,Wang Y,Du Y,Song S,Li J,Su Z,Shi Y,Wu H

Sun Jun 11 00:00:00 EDT 2023

Irisin Ameliorates Renal Tubulointerstitial Fibrosis by Regulating the Smad4/β-Catenin Pathway in Diabetic Mice.

Diabetes, metabolic syndrome and obesity : targets and therapy

Yang Z,Wei J,Wang Y,Du Y,Song S,Li J,Su Z,Shi Y,Wu H

Sun Jun 11 00:00:00 EDT 2023

Agmatinase facilitates the tumorigenesis of pancreatic adenocarcinoma through the TGFβ/Smad pathway.

Experimental and therapeutic medicine

Zhang Y,Cao L,Xie Y,Wang C,Liu X,Zhang X,Chen J

Mon Aug 01 00:00:00 EDT 2022

Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Wu L,Yang FR,Xing ML,Lu SF,Chen HL,Yang QW,Zhang YT,Lu Y,Huang Y

Wed Jun 01 00:00:00 EDT 2022

Gli^ko BMSC: A potential strategy of treatment for renal fibrosis.

Regenerative therapy

Shi L,Gao X,Bi Y,Li M,Sun H,Tian X,Bi W

Wed Jun 01 00:00:00 EDT 2022

Krill Oil Turns Off TGF-β1 Profibrotic Signaling in the Prevention of Diabetic Nephropathy.

Journal of agricultural and food chemistry

Sun X,Yang Y,Sun X,Meng H,Hao W,Yin J,Ma F,Guo X,Du L,Sun L,Wu H

Wed Aug 17 00:00:00 EDT 2022

SMAD4 Inhibits Granulosa Cell Apoptosis via the miR-183-96-182 Cluster and FoxO1 Axis.

Reproductive sciences (Thousand Oaks, Calif.)

Yao W,Wang S,Du X,Lin C,Zhang J,Pan Z,Li Q

Sun May 01 00:00:00 EDT 2022

Nicotinamide N-methyltransferase ameliorates renal fibrosis by its metabolite 1-methylnicotinamide inhibiting the TGF-β1/Smad3 pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang W,Rong G,Gu J,Fan C,Guo T,Jiang T,Deng W,Xie J,Su Z,Yu Q,Mai J,Zheng R,Chen X,Tang X,Zhang J

Tue Mar 01 00:00:00 EST 2022

The Photoperiod Regulates Granulosa Cell Apoptosis through the FSH-Nodal/ALK7 Signaling Pathway in Phodopus sungorus.

Animals : an open access journal from MDPI

Qi Y,Xue HL,Zheng YJ,Yin YF,Xu WL,Xu JH,Wu M,Chen L,Xu LX

Fri Dec 16 00:00:00 EST 2022

TRIM47 promotes glioma angiogenesis by suppressing Smad4.

In vitro cellular & developmental biology. Animal

Wang Z,Li Z,Han C,Cheng Y,Wang K

Sat Oct 01 00:00:00 EDT 2022

Post-translational control of beige fat biogenesis by PRDM16 stabilization.

Nature

Wang Q,Li H,Tajima K,Verkerke ARP,Taxin ZH,Hou Z,Cole JB,Li F,Wong J,Abe I,Pradhan RN,Yamamuro T,Yoneshiro T,Hirschhorn JN,Kajimura S

Thu Sep 01 00:00:00 EDT 2022

Bu-Gu-Sheng-Sui decoction promotes osteogenesis via activating the ERK/Smad signaling pathways.

Frontiers in pharmacology

Liu N,Qi B,Zhang Y,Fang S,Sun C,Li Q,Wei X

Tue Sep 13 00:00:00 EDT 2022

Systematic Survey of the Regulatory Networks of the Long Noncoding RNA BANCR in Cervical Cancer Cells.

Journal of proteome research

Wang B,Wang M,Jia S,Li T,Yang M,Ge F

Fri Apr 01 00:00:00 EDT 2022

Apigenin inhibits fibrous scar formation after acute spinal cord injury through TGFβ/SMADs signaling pathway.

CNS neuroscience & therapeutics

Jin Z,Tian L,Zhang Y,Zhang X,Kang J,Dong H,Huang N,Pan L,Ning B

Tue Nov 01 00:00:00 EDT 2022

Smad7 Is Highly Expressed in Human Degenerative Discs and Participates in IL-1β-Induced Apoptosis of Rat AF Cells via the Mitochondria Pathway.

Oxidative medicine and cellular longevity

Li B,Lu ZY,Jiang SD,Jiang LS,Zheng XF

Fri Jul 08 00:00:00 EDT 2022

SMAD4 Feedback Activates the Canonical TGF-β Family Signaling Pathways.

International journal of molecular sciences

Liu L,Li Q,Yang L,Li Q,Du X

Thu Sep 16 00:00:00 EDT 2021

SMAD4 Feedback Activates the Canonical TGF-β Family Signaling Pathways.

International journal of molecular sciences

Liu L,Li Q,Yang L,Li Q,Du X

Thu Sep 16 00:00:00 EDT 2021

Pilose Antler Peptide-3.2KD Ameliorates Adriamycin-Induced Myocardial Injury Through TGF-β/SMAD Signaling Pathway.

Frontiers in cardiovascular medicine

Xu Y,Qu X,Zhou J,Lv G,Han D,Liu J,Liu Y,Chen Y,Qu P,Huang X

Tue Jun 15 00:00:00 EDT 2021

A mesenchymal-like subpopulation in non-neuroendocrine SCLC contributes to metastasis.

Journal of genetics and genomics = Yi chuan xue bao

Jin Y,Xiao T,Feng Y,Yang J,Guo C,Hu L,Ji H

Tue Jul 20 00:00:00 EDT 2021

FOXM1-induced miR-552 expression contributes to pancreatic cancer progression by targeting multiple tumor suppressor genes.

International journal of biological sciences

Wang X,Dou N,Wang J,Zhang Y,Li Y,Gao Y

Fri Jan 21 00:00:00 EST 2022

SEPHS1 promotes SMAD2/3/4 expression and hepatocellular carcinoma cells invasion.

Experimental hematology & oncology

Yang S,Zhang H,Yang H,Zhang J,Wang J,Luo T,Jiang Y,Hua H

Tue Feb 23 00:00:00 EST 2021

Hsa_circ_0001021 regulates intestinal epithelial barrier function via sponging miR-224-5p in ulcerative colitis.

Epigenomics

Li B,Li Y,Li L,Yu Y,Gu X,Liu C,Long X,Yu Y,Zuo X

Wed Sep 01 00:00:00 EDT 2021

The Jumonji Domain-Containing Histone Demethylase Homolog 1D/lysine Demethylase 7A (JHDM1D/KDM7A) Is an Epigenetic Activator of RHOJ Transcription in Breast Cancer Cells.

Frontiers in cell and developmental biology

Zhang Z,Chen B,Zhu Y,Zhang T,Zhang X,Yuan Y,Xu Y

Thu Sep 23 00:00:00 EDT 2021

The Jumonji Domain-Containing Histone Demethylase Homolog 1D/lysine Demethylase 7A (JHDM1D/KDM7A) Is an Epigenetic Activator of RHOJ Transcription in Breast Cancer Cells.

Frontiers in cell and developmental biology

Zhang Z,Chen B,Zhu Y,Zhang T,Zhang X,Yuan Y,Xu Y

Thu Sep 23 00:00:00 EDT 2021

MiR-664-3p suppresses osteoblast differentiation and impairs bone formation via targeting Smad4 and Osterix.

Journal of cellular and molecular medicine

Xu Y,Jin Y,Hong F,Ma Y,Yang J,Tang Y,Zhu Z,Wu J,Bao Q,Li L,Yao B,Li D,Ma C

Tue Jun 01 00:00:00 EDT 2021

Smad4 deficiency substitutes Cdkn2b but not Cdkn2a downregulation in pancreatic cancer following induction of genetic events in adult mice.

Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]

Jia X,Sun B,Tu Q,Qi H,Li L,Liu X,Yan L,Dai H,Kong Q,Tang C,Zhao X

Mon Mar 01 00:00:00 EST 2021

Huangqi Shengmai Yin Ameliorates Myocardial Fibrosis by Activating Sirtuin3 and Inhibiting TGF-β/Smad Pathway.

Frontiers in pharmacology

Pan J,Cao Z,Fang C,Lei Y,Sun J,Huang X,Han D

Tue Sep 07 00:00:00 EDT 2021

Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells.

Translational research : the journal of laboratory and clinical medicine

Liao X,Li Y,Liu J,Zhang Y,Tan J,Kass DJ,Rojas M,Mallampalli RK,Zhao J,Zhao Y

Tue Sep 01 00:00:00 EDT 2020

Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells.

Translational research : the journal of laboratory and clinical medicine

Liao X,Li Y,Liu J,Zhang Y,Tan J,Kass DJ,Rojas M,Mallampalli RK,Zhao J,Zhao Y

Tue Sep 01 00:00:00 EDT 2020

Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells.

Translational research : the journal of laboratory and clinical medicine

Liao X,Li Y,Liu J,Zhang Y,Tan J,Kass DJ,Rojas M,Mallampalli RK,Zhao J,Zhao Y

Tue Sep 01 00:00:00 EDT 2020

Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2.

Cells

Ding Q,Jin M,Wang Y,Liu J,Kalds P,Wang Y,Yang Y,Wang X,Chen Y

Fri Feb 14 00:00:00 EST 2020

Novel Alternatively Spliced Variants of Smad4 Expressed in TGF-β-Induced EMT Regulating Proliferation and Migration of A549 Cells.

OncoTargets and therapy

Wan R,Xu X,Ma L,Chen Y,Tang L,Feng J

Wed Apr 13 00:00:00 EDT 2022

Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model.

Stem cell research & therapy

Cui Q,Zhang D,Kong D,Tang J,Liao X,Yang Q,Ren J,Gong Y,Wu G

Tue May 26 00:00:00 EDT 2020

KDM6A suppresses hepatocellular carcinoma cell proliferation by negatively regulating the TGF-β/SMAD signaling pathway.

Experimental and therapeutic medicine

Li Y,Yang J,Zhang X,Liu H,Guo J

Tue Sep 01 00:00:00 EDT 2020

Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.

Aging

Sha L,Ma D,Chen C

Sun Dec 13 00:00:00 EST 2020

Trimetazidine Inhibits Renal Tubular Epithelial Cells to Mesenchymal Transition in Diabetic Rats via Upregulation of Sirt1.

Frontiers in pharmacology

Yang Y,Wang Y,He Z,Liu Y,Chen C,Wang Y,Wang DW,Wang H

Mon Sep 28 00:00:00 EDT 2020

Si-Miao-Yong-An decoction attenuates cardiac fibrosis via suppressing TGF-β1 pathway and interfering with MMP-TIMPs expression.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Su C,Wang Q,Luo H,Jiao W,Tang J,Li L,Tian L,Chen X,Liu B,Yu X,Li S,Guo S,Wang W

Wed Jul 01 00:00:00 EDT 2020

Comparison of anticarcinogenic properties of Viburnum opulus and its active compound p-coumaric acid on human colorectal carcinoma.

Turkish journal of biology = Turk biyoloji dergisi

Karakurt S,AbuŞo¿lu G,Arituluk ZC

Thu Oct 29 00:00:00 EDT 2020

Small airway remodeling in diabetic and smoking chronic obstructive pulmonary disease patients.

Aging

Wu N,Wu Z,Sun J,Yan M,Wang B,Du X,Liu Y

Tue May 05 00:00:00 EDT 2020

Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.

Scientific reports

Wang X,Liao X,Zhang Y,Wei L,Pang Y

Wed Sep 02 00:00:00 EDT 2020

β2-microglobulin as a biomarker of pulmonary fibrosis development in COPD patients.

Aging

Wu Z,Yan M,Zhang M,Wu N,Ma G,Wang B,Fan Y,Du X,Ding C,Liu Y

Mon Dec 21 00:00:00 EST 2020

Myostatin/SMAD4 signaling-mediated regulation of miR-124-3p represses glucocorticoid receptor expression and inhibits adipocyte differentiation.

American journal of physiology. Endocrinology and metabolism

Liu K,Zhang X,Wei W,Liu X,Tian Y,Han H,Zhang L,Wu W,Chen J

Mon Apr 01 00:00:00 EDT 2019

Myostatin/SMAD4 signaling-mediated regulation of miR-124-3p represses glucocorticoid receptor expression and inhibits adipocyte differentiation.

American journal of physiology. Endocrinology and metabolism

Liu K,Zhang X,Wei W,Liu X,Tian Y,Han H,Zhang L,Wu W,Chen J

Mon Apr 01 00:00:00 EDT 2019

Myostatin/SMAD4 signaling-mediated regulation of miR-124-3p represses glucocorticoid receptor expression and inhibits adipocyte differentiation.

American journal of physiology. Endocrinology and metabolism

Liu K,Zhang X,Wei W,Liu X,Tian Y,Han H,Zhang L,Wu W,Chen J

Mon Apr 01 00:00:00 EDT 2019

Myostatin/SMAD4 signaling-mediated regulation of miR-124-3p represses glucocorticoid receptor expression and inhibits adipocyte differentiation.

American journal of physiology. Endocrinology and metabolism

Liu K,Zhang X,Wei W,Liu X,Tian Y,Han H,Zhang L,Wu W,Chen J

Mon Apr 01 00:00:00 EDT 2019

Inhibition of Skp1-Cullin-F-box complexes during bovine oocyte maturation and preimplantation development leads to delayed development of embryos†.

Biology of reproduction

Kinterova V,Kanka J,Petruskova V,Toralova T

Mon Apr 01 00:00:00 EDT 2019

The role and mechanism of transforming growth factor beta 3 in human myocardial infarction-induced myocardial fibrosis.

Journal of cellular and molecular medicine

Xue K,Zhang J,Li C,Li J,Wang C,Zhang Q,Chen X,Yu X,Sun L,Yu X

Sat Jun 01 00:00:00 EDT 2019

Overexpression of matrix metalloproteinase-9 in breast cancer cell lines remarkably increases the cell malignancy largely via activation of transforming growth factor beta/SMAD signalling.

Cell proliferation

Dong H,Diao H,Zhao Y,Xu H,Pei S,Gao J,Wang J,Hussain T,Zhao D,Zhou X,Lin D

Sun Sep 01 00:00:00 EDT 2019

Oviductus Ranae protein hydrolyzate prevents menopausal osteoporosis by regulating TGFβ/BMP2 signaling.

Archives of gynecology and obstetrics

Li X,Sui X,Yang Q,Li Y,Li N,Shi X,Han D,Li Y,Huang X,Yu P,Qu X

Fri Mar 01 00:00:00 EST 2019

miR-93-5p targets Smad7 to regulate the transforming growth factor-β1/Smad3 pathway and mediate fibrosis in drug-resistant prolactinoma.

Brain research bulletin

Hu B,Mao Z,Du Q,Jiang X,Wang Z,Xiao Z,Zhu D,Wang X,Zhu Y,Wang H

Mon Jul 01 00:00:00 EDT 2019

MicroRNA-140 Inhibits the Epithelial-Mesenchymal Transition and Metastasis in Colorectal Cancer.

Molecular therapy. Nucleic acids

Li J,Zou K,Yu L,Zhao W,Lu Y,Mao J,Wang B,Wang L,Fan S,Song B,Li L

Fri Mar 02 00:00:00 EST 2018

MicroRNA-140 Inhibits the Epithelial-Mesenchymal Transition and Metastasis in Colorectal Cancer.

Molecular therapy. Nucleic acids

Li J,Zou K,Yu L,Zhao W,Lu Y,Mao J,Wang B,Wang L,Fan S,Song B,Li L

Fri Mar 02 00:00:00 EST 2018

Inhibition of lysyl oxidase expression by dextran sulfate affects invasion and migration of gastric cancer cells.

International journal of molecular medicine

Xu Y,Wang X,Huang Y,Ma Y,Jin X,Wang H,Wang J

Thu Nov 01 00:00:00 EDT 2018

Inhibition of lysyl oxidase expression by dextran sulfate affects invasion and migration of gastric cancer cells.

International journal of molecular medicine

Xu Y,Wang X,Huang Y,Ma Y,Jin X,Wang H,Wang J

Thu Nov 01 00:00:00 EDT 2018

Role of TGF-β1/Smad3-mediated fibrosis in drug resistance mechanism of prolactinoma.

Brain research

Hu B,Mao Z,Jiang X,He D,Wang Z,Wang X,Zhu Y,Wang H

Thu Nov 01 00:00:00 EDT 2018

Role of TGF-β1/Smad3-mediated fibrosis in drug resistance mechanism of prolactinoma.

Brain research

Hu B,Mao Z,Jiang X,He D,Wang Z,Wang X,Zhu Y,Wang H

Thu Nov 01 00:00:00 EDT 2018

Role of TGF-β1/Smad3-mediated fibrosis in drug resistance mechanism of prolactinoma.

Brain research

Hu B,Mao Z,Jiang X,He D,Wang Z,Wang X,Zhu Y,Wang H

Thu Nov 01 00:00:00 EDT 2018

Role of TGF-β1/Smad3-mediated fibrosis in drug resistance mechanism of prolactinoma.

Brain research

Hu B,Mao Z,Jiang X,He D,Wang Z,Wang X,Zhu Y,Wang H

Thu Nov 01 00:00:00 EDT 2018

Inhibiting transforming growth factor-β signaling regulates in vitro maintenance and differentiation of bovine bone marrow mesenchymal stem cells.

Journal of experimental zoology. Part B, Molecular and developmental evolution

Zhao XX,An XL,Zhu XC,Jiang Y,Zhai YH,Zhang S,Cai NN,Tang B,Li ZY,Zhang XM

Sat Dec 01 00:00:00 EST 2018

Inhibiting transforming growth factor-β signaling regulates in vitro maintenance and differentiation of bovine bone marrow mesenchymal stem cells.

Journal of experimental zoology. Part B, Molecular and developmental evolution

Zhao XX,An XL,Zhu XC,Jiang Y,Zhai YH,Zhang S,Cai NN,Tang B,Li ZY,Zhang XM

Sat Dec 01 00:00:00 EST 2018

SIRT7 suppresses the epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis by promoting SMAD4 deacetylation.

Journal of experimental & clinical cancer research : CR

Li W,Zhu D,Qin S

Fri Jul 13 00:00:00 EDT 2018

SIRT7 suppresses the epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis by promoting SMAD4 deacetylation.

Journal of experimental & clinical cancer research : CR

Li W,Zhu D,Qin S

Fri Jul 13 00:00:00 EDT 2018

SIRT7 suppresses the epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis by promoting SMAD4 deacetylation.

Journal of experimental & clinical cancer research : CR

Li W,Zhu D,Qin S

Fri Jul 13 00:00:00 EDT 2018

Gene Correction Reverses Ciliopathy and Photoreceptor Loss in iPSC-Derived Retinal Organoids from Retinitis Pigmentosa Patients.

Stem cell reports

Deng WL,Gao ML,Lei XL,Lv JN,Zhao H,He KW,Xia XX,Li LY,Chen YC,Li YP,Pan D,Xue T,Jin ZB

Tue Apr 10 00:00:00 EDT 2018

Gene Correction Reverses Ciliopathy and Photoreceptor Loss in iPSC-Derived Retinal Organoids from Retinitis Pigmentosa Patients.

Stem cell reports

Deng WL,Gao ML,Lei XL,Lv JN,Zhao H,He KW,Xia XX,Li LY,Chen YC,Li YP,Pan D,Xue T,Jin ZB

Tue Apr 10 00:00:00 EDT 2018

Transcription coactivator Cited1 acts as an inducer of trophoblast-like state from mouse embryonic stem cells through the activation of BMP signaling.

Cell death & disease

Xu Y,Luo X,Fang Z,Zheng X,Zeng Y,Zhu C,Gu J,Tang F,Hu Y,Hu G,Jin Y,Li H

Tue Sep 11 00:00:00 EDT 2018

Transcription coactivator Cited1 acts as an inducer of trophoblast-like state from mouse embryonic stem cells through the activation of BMP signaling.

Cell death & disease

Xu Y,Luo X,Fang Z,Zheng X,Zeng Y,Zhu C,Gu J,Tang F,Hu Y,Hu G,Jin Y,Li H

Tue Sep 11 00:00:00 EDT 2018

Lnc-ATB contributes to gastric cancer growth through a MiR-141-3p/TGFβ2 feedback loop.

Biochemical and biophysical research communications

Lei K,Liang X,Gao Y,Xu B,Xu Y,Li Y,Tao Y,Shi W,Liu J

Sat Mar 11 00:00:00 EST 2017

Lnc-ATB contributes to gastric cancer growth through a MiR-141-3p/TGFβ2 feedback loop.

Biochemical and biophysical research communications

Lei K,Liang X,Gao Y,Xu B,Xu Y,Li Y,Tao Y,Shi W,Liu J

Sat Mar 11 00:00:00 EST 2017

Decrease of FSTL1-BMP4-Smad signaling predicts poor prognosis in lung adenocarcinoma but not in squamous cell carcinoma.

Scientific reports

Chiou J,Su CY,Jan YH,Yang CJ,Huang MS,Yu YL,Hsiao M

Tue Aug 29 00:00:00 EDT 2017

Decrease of FSTL1-BMP4-Smad signaling predicts poor prognosis in lung adenocarcinoma but not in squamous cell carcinoma.

Scientific reports

Chiou J,Su CY,Jan YH,Yang CJ,Huang MS,Yu YL,Hsiao M

Tue Aug 29 00:00:00 EDT 2017

TGFβ1 Promotes Gemcitabine Resistance through Regulating the LncRNA-LET/NF90/miR-145 Signaling Axis in Bladder Cancer.

Theranostics

Zhuang J,Shen L,Yang L,Huang X,Lu Q,Cui Y,Zheng X,Zhao X,Zhang D,Huang R,Guo H,Yan J

Mon Apr 30 00:00:00 EDT 2018

TGFβ1 Promotes Gemcitabine Resistance through Regulating the LncRNA-LET/NF90/miR-145 Signaling Axis in Bladder Cancer.

Theranostics

Zhuang J,Shen L,Yang L,Huang X,Lu Q,Cui Y,Zheng X,Zhao X,Zhang D,Huang R,Guo H,Yan J

Mon Apr 30 00:00:00 EDT 2018

Let‑7a suppresses cell proliferation via the TGF‑β/SMAD signaling pathway in cervical cancer.

Oncology reports

Wu T,Chen X,Peng R,Liu H,Yin P,Peng H,Zhou Y,Sun Y,Wen L,Yi H,Li A,Zhang Z

Thu Dec 01 00:00:00 EST 2016

Let‑7a suppresses cell proliferation via the TGF‑β/SMAD signaling pathway in cervical cancer.

Oncology reports

Wu T,Chen X,Peng R,Liu H,Yin P,Peng H,Zhou Y,Sun Y,Wen L,Yi H,Li A,Zhang Z

Thu Dec 01 00:00:00 EST 2016

Prognostic Value of SMAD4 in Pancreatic Cancer: A Meta-Analysis.

Translational oncology

Shugang X,Hongfa Y,Jianpeng L,Xu Z,Jingqi F,Xiangxiang L,Wei L

Mon Feb 01 00:00:00 EST 2016

Anti-fibrotic effects of neferine on carbon tetrachloride-induced hepatic fibrosis in mice.

The American journal of Chinese medicine

Chen MS,Zhang JH,Wang JL,Gao L,Chen XX,Xiao JH

Thu Jan 14 00:00:00 EST 2016

Anti-fibrotic effects of neferine on carbon tetrachloride-induced hepatic fibrosis in mice.

The American journal of Chinese medicine

Chen MS,Zhang JH,Wang JL,Gao L,Chen XX,Xiao JH

Thu Jan 14 00:00:00 EST 2016

Biomarkers of TGF-β signaling pathway and prognosis of pancreatic cancer.

PloS one

Javle M,Li Y,Tan D,Dong X,Chang P,Kar S,Li D

Fri Jan 23 00:00:00 EST 2015

Biomarkers of TGF-β signaling pathway and prognosis of pancreatic cancer.

PloS one

Javle M,Li Y,Tan D,Dong X,Chang P,Kar S,Li D

Fri Jan 23 00:00:00 EST 2015

Aberrant elevated microRNA-146a in dendritic cells (DC) induced by human pancreatic cancer cell line BxPC-3-conditioned medium inhibits DC maturation and activation.

Medical oncology (Northwood, London, England)

Du J,Wang J,Tan G,Cai Z,Zhang L,Tang B,Wang Z

Sat Dec 01 00:00:00 EST 2012

Aberrant elevated microRNA-146a in dendritic cells (DC) induced by human pancreatic cancer cell line BxPC-3-conditioned medium inhibits DC maturation and activation.

Medical oncology (Northwood, London, England)

Du J,Wang J,Tan G,Cai Z,Zhang L,Tang B,Wang Z

Sat Dec 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: Deleted in Pancreatic Carcinoma 4 (DPC4); deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; Deletion target in pancreatic carcinoma 4 homolog; hSMAD4; MAD (mothers against decapentaplegic Drosophila) homolog 4; MAD homolog 4; MAD homolog 4 (MADH4); Mothers against decapentaplegic homolog 4; Mothers against decapentaplegic homolog 4 (MADH4); mothers against decapentaplegic, Drosophila, homolog of, 4; mothers against DPP homolog 4; SMAD; SMAD 4; SMAD family member 4; SMAD, mothers against DPP homolog 4

View more View less

Gene Aliases: AW743858; D18Wsu70e; DPC4; JIP; MADH4; MYHRS; SMAD4

View more View less

UniProt ID: (Human) Q13485, (Mouse) P97471, (Rat) O70437

View more View less

Entrez Gene ID: (Human) 4089, (Mouse) 17128, (Rat) 50554

View more View less

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcription factor activity, protein binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding collagen binding transforming growth factor beta receptor, common-partner cytoplasmic mediator activity identical protein binding protein homodimerization activity sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding protein heterodimerization activity I-SMAD binding R-SMAD binding RNA polymerase II regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding filamin binding SMAD binding DNA-binding transcription factor gene-specific transcriptional regulator

Process(es)

negative regulation of transcription from RNA polymerase II promoter ovarian follicle development branching involved in ureteric bud morphogenesis response to hypoxia in utero embryonic development gastrulation with mouth forming second atrioventricular valve formation epithelial to mesenchymal transition involved in endocardial cushion formation left ventricular cardiac muscle tissue morphogenesis positive regulation of cell proliferation involved in heart valve morphogenesis brainstem development transcription from RNA polymerase II promoter cellular iron ion homeostasis transforming growth factor beta receptor signaling pathway SMAD protein complex assembly spermatogenesis single fertilization axon guidance endoderm development mesoderm development cell proliferation negative regulation of cell proliferation negative regulation of cardiac muscle hypertrophy positive regulation of epithelial to mesenchymal transition positive regulation of pathway-restricted SMAD protein phosphorylation neural crest cell differentiation regulation of transforming growth factor beta receptor signaling pathway negative regulation of cell growth BMP signaling pathway positive regulation of transforming growth factor beta receptor signaling pathway positive regulation of BMP signaling pathway somite rostral/caudal axis specification regulation of transforming growth factor beta2 production positive regulation of luteinizing hormone secretion somatic stem cell population maintenance intracellular signal transduction atrioventricular canal development endothelial cell activation embryonic digit morphogenesis negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of follicle-stimulating hormone secretion developmental growth neuron fate commitment sebaceous gland development formation of anatomical boundary regulation of binding positive regulation of histone H3-K4 methylation regulation of hair follicle development palate development uterus development positive regulation of SMAD protein import into nucleus SMAD protein signal transduction ventricular septum morphogenesis negative regulation of cell death endocardial cell differentiation female gonad morphogenesis interleukin-6-mediated signaling pathway negative regulation of ERK1 and ERK2 cascade response to transforming growth factor beta cellular response to BMP stimulus metanephric mesenchyme morphogenesis nephrogenic mesenchyme morphogenesis seminiferous tubule development positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus positive regulation of histone H3-K9 acetylation kidney development cardiac septum development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter gastrulation anterior/posterior pattern specification regulation of cell proliferation negative regulation of protein catabolic process tissue morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

SMAD4 Polyclonal Antibody

SMAD4 Antibody (10231-1-AP) in IHC (P)

Product Details

10231-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!