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SMAD4 Antibody (PA5-35330) in ICC/IF
Immunocytochemistry analysis of SMAD4 in HeLa cells. Samples were incubated with SMAD4 polyclonal antibody (Product # PA5-35330) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min). SMAD4... View More
Product Details
PA5-35330
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 400-428 amino acids from the C-terminal region of human SMAD4
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2552640
Product Specific Information
This antibody is predicted to react with bovine, mouse, porcine and rat based on sequence homology.
Target Information
Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-related protein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deleted in Pancreatic Carcinoma 4 (DPC4); deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; hSMAD4; MAD homolog 4; MAD homolog 4 (MADH4); Mothers against decapentaplegic homolog 4; Mothers against decapentaplegic homolog 4 (MADH4); mothers against decapentaplegic, Drosophila, homolog of, 4; SMAD; SMAD 4; SMAD family member 4; SMAD, mothers against DPP homolog 4
Gene Aliases: DPC4; JIP; MADH4; MYHRS; SMAD4
UniProt ID: (Human) Q13485
Entrez Gene ID: (Human) 4089
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcription factor activity, protein binding
transcription factor activity, RNA polymerase II transcription factor binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
collagen binding
transforming growth factor beta receptor, common-partner cytoplasmic mediator activity
identical protein binding
protein homodimerization activity
sequence-specific DNA binding
transcription regulatory region DNA binding
metal ion binding
protein heterodimerization activity
I-SMAD binding
R-SMAD binding
DNA-binding transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
ovarian follicle development
branching involved in ureteric bud morphogenesis
response to hypoxia
in utero embryonic development
gastrulation with mouth forming second
atrioventricular valve formation
epithelial to mesenchymal transition involved in endocardial cushion formation
left ventricular cardiac muscle tissue morphogenesis
positive regulation of cell proliferation involved in heart valve morphogenesis
brainstem development
transcription from RNA polymerase II promoter
cellular iron ion homeostasis
transforming growth factor beta receptor signaling pathway
SMAD protein complex assembly
spermatogenesis
single fertilization
axon guidance
endoderm development
mesoderm development
cell proliferation
negative regulation of cell proliferation
negative regulation of cardiac muscle hypertrophy
positive regulation of epithelial to mesenchymal transition
positive regulation of pathway-restricted SMAD protein phosphorylation
neural crest cell differentiation
regulation of transforming growth factor beta receptor signaling pathway
negative regulation of cell growth
BMP signaling pathway
positive regulation of transforming growth factor beta receptor signaling pathway
positive regulation of BMP signaling pathway
somite rostral/caudal axis specification
regulation of transforming growth factor beta2 production
positive regulation of luteinizing hormone secretion
somatic stem cell population maintenance
intracellular signal transduction
atrioventricular canal development
endothelial cell activation
embryonic digit morphogenesis
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of follicle-stimulating hormone secretion
developmental growth
neuron fate commitment
sebaceous gland development
formation of anatomical boundary
regulation of binding
positive regulation of histone H3-K4 methylation
regulation of hair follicle development
palate development
uterus development
positive regulation of SMAD protein import into nucleus
SMAD protein signal transduction
ventricular septum morphogenesis
negative regulation of cell death
endocardial cell differentiation
female gonad morphogenesis
interleukin-6-mediated signaling pathway
negative regulation of ERK1 and ERK2 cascade
response to transforming growth factor beta
cellular response to BMP stimulus
metanephric mesenchyme morphogenesis
nephrogenic mesenchyme morphogenesis
seminiferous tubule development
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
positive regulation of histone H3-K9 acetylation
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