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NeoBiotechnologies
SMAD4 (Pancreatic Adenocarcinoma Marker) Monoclonal Antibody (SMAD4/2524)
Product Details
4089-MSM2-P1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
SMAD4/2524
Immunogen
Recombinant full-length human STAT6 protein
Conjugate
Form
Liquid
Concentration
200 µg/mL
Purification
Protein A/G
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Antibody is stable for 24 months.
Positive Control: Jurkat or A431 cells. Pancreatic Carcinoma Cellular Localization: Cytoplasmic & Nuclear
Specificity Comments: Signaling from the ligand-activated membrane receptor serine/threonine kinases to nuclear targets is mediated by a set of evolutionarily conserved proteins known as DPC4. Upon ligand binding, the receptors of the TGF- family phosphorylate SMAD proteins (SMAD1 and SMAD2). These proteins then move into the nucleus, where they activate transcription. To carry out this function, the receptor activated SMAD1 and 2 require association with the product of deleted in pancreatic carcinoma, locus 4 (DPC4), also known as SMAD4. SMAD4/DPC4 is also implicated as a tumor suppressor, since it is inactivated in more than half of pancreatic carcinomas and to a lesser extent in a variety of other cancers. SMAD4 is absent in approximately 80% of pancreatic adeno carcinoma, but rarely in endometrial, colorectal, ovarian, lung, breast adeno carcinomas, and malignant melanom. SMAD4 is an important marker for confirming a diagnosis of pancreatic adeno carcinoma. Patients with pancreatic adeno carcinomas with SMAD4 protein expression had significantly longer survival than SMAD4 negative patients.
Target Information
Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-related protein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deleted in Pancreatic Carcinoma 4 (DPC4); deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; hSMAD4; MAD homolog 4; MAD homolog 4 (MADH4); Mothers against decapentaplegic homolog 4; Mothers against decapentaplegic homolog 4 (MADH4); mothers against decapentaplegic, Drosophila, homolog of, 4; SMAD; SMAD 4; SMAD family member 4; SMAD, mothers against DPP homolog 4
Gene Aliases: DPC4; JIP; MADH4; MYHRS; SMAD4
UniProt ID: (Human) Q13485
Entrez Gene ID: (Human) 4089
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcription factor activity, protein binding
transcription factor activity, RNA polymerase II transcription factor binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
collagen binding
transforming growth factor beta receptor, common-partner cytoplasmic mediator activity
identical protein binding
protein homodimerization activity
sequence-specific DNA binding
transcription regulatory region DNA binding
metal ion binding
protein heterodimerization activity
I-SMAD binding
R-SMAD binding
DNA-binding transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
ovarian follicle development
branching involved in ureteric bud morphogenesis
response to hypoxia
in utero embryonic development
gastrulation with mouth forming second
atrioventricular valve formation
epithelial to mesenchymal transition involved in endocardial cushion formation
left ventricular cardiac muscle tissue morphogenesis
positive regulation of cell proliferation involved in heart valve morphogenesis
brainstem development
transcription from RNA polymerase II promoter
cellular iron ion homeostasis
transforming growth factor beta receptor signaling pathway
SMAD protein complex assembly
spermatogenesis
single fertilization
axon guidance
endoderm development
mesoderm development
cell proliferation
negative regulation of cell proliferation
negative regulation of cardiac muscle hypertrophy
positive regulation of epithelial to mesenchymal transition
positive regulation of pathway-restricted SMAD protein phosphorylation
neural crest cell differentiation
regulation of transforming growth factor beta receptor signaling pathway
negative regulation of cell growth
BMP signaling pathway
positive regulation of transforming growth factor beta receptor signaling pathway
positive regulation of BMP signaling pathway
somite rostral/caudal axis specification
regulation of transforming growth factor beta2 production
positive regulation of luteinizing hormone secretion
somatic stem cell population maintenance
intracellular signal transduction
atrioventricular canal development
endothelial cell activation
embryonic digit morphogenesis
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of follicle-stimulating hormone secretion
developmental growth
neuron fate commitment
sebaceous gland development
formation of anatomical boundary
regulation of binding
positive regulation of histone H3-K4 methylation
regulation of hair follicle development
palate development
uterus development
positive regulation of SMAD protein import into nucleus
SMAD protein signal transduction
ventricular septum morphogenesis
negative regulation of cell death
endocardial cell differentiation
female gonad morphogenesis
interleukin-6-mediated signaling pathway
negative regulation of ERK1 and ERK2 cascade
response to transforming growth factor beta
cellular response to BMP stimulus
metanephric mesenchyme morphogenesis
nephrogenic mesenchyme morphogenesis
seminiferous tubule development
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
positive regulation of histone H3-K9 acetylation
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