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SMAD9 Antibody (PA5-81546) in ICC/IF
Immunofluorescence staining of SMAD9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with SMAD9 Polyclonal Antibody (Product # PA5-81546, 1:5,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Product Details
PA5-81546
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the center region of the Human SMAD9
Conjugate
Form
Liquid
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2788749
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human SMAD9.
Target Information
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. Mutations of this gene can lead to pulmonary hypertension, primary 2 (PPH2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:6774; MAD; MAD homolog 9; Madh6; Mothers against decapentaplegic homolog 9; Mothers against decapentaplegic, drosophila, homolog of, 9; RP11-421P11.9; SMAD; SMAD 9; SMAD family member 9; SMAD, mothers against DPP homolog 9
Gene Aliases: MADH6; MADH9; PPH2; SMAD8; SMAD8/9; SMAD8A; SMAD8B; SMAD9
UniProt ID: (Human) O15198
Entrez Gene ID: (Human) 4093
ureteric bud development
transcription, DNA-templated
protein phosphorylation
transforming growth factor beta receptor signaling pathway
BMP signaling pathway
midbrain development
hindbrain development
cartilage development
bone development
SMAD protein signal transduction
cellular response to organic cyclic compound
cellular response to BMP stimulus
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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