Proteintech

SMO Polyclonal Antibody

SMO Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SMO Antibody (20787-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using (Product # 20787-1-AP) (SMO antibody) at dilution of 1:1,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

20787-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

View 23 publications 23 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SMO Fusion Protein Ag14322 (546-787 aa encoded by BC009989)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.44 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: RRTWCRLTG QSDDEPKRIK KSKMIAKAFS KRHELLQNPG QELSFSMHTV SHDGPVAGLA FDLNEPSADV SSAWAQHVTK MVARRGAILP QDISVTPVAT PVPPEEQANL WLVEAEISPE LQKRLGRKKK RRKRKKEVCP LAPPPELHPP APAPSTIPRL PQLPRQKCLV AAGAWGAGDS CRQGAWTLVS NPFCPEPSPP QDPFLPSAPA PVAWAHGRRQ GLGPIHSRTN LMDTELMDAD SDF (546-787 aa encoded by BC009989)

Target Information

Smoothened drosophila homolog (SMOH) is a serpentine protein belonging to the G-protein coupled receptor Fz/Smo family with 787 aa, seven transmembrane G protein-coupled receptors with 4 glycosylation sites and a putative extracellular amino terminus. Smoothened activated by b-Arrestin 2 and GRK2 acts as a receptor for Shh, the Sonic hedgehog gene product, and is a positive regulator of hedgehog signaling pathway along with the smoothened inhibitor patched protein (PTCH) and regulates vertebrate developmental processes, cellular proliferation and differentiation by activating transcription factor Gli. Smoothened mutants are known to cause sporadic basal cell carcinoma of the skin. It is known to express widely in heart and brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Sat May 06 00:00:00 EDT 2023

Low-dose phthalates promote breast cancer stem cell properties via the oncogene ΔNp63α and the Sonic hedgehog pathway.

Ecotoxicology and environmental safety

Cao WS,Zhao MJ,Chen Y,Zhu JY,Xie CF,Li XT,Geng SS,Zhong CY,Fu JY,Wu JS

Wed Mar 01 00:00:00 EST 2023

Low-dose phthalates promote breast cancer stem cell properties via the oncogene ΔNp63α and the Sonic hedgehog pathway.

Ecotoxicology and environmental safety

Cao WS,Zhao MJ,Chen Y,Zhu JY,Xie CF,Li XT,Geng SS,Zhong CY,Fu JY,Wu JS

Wed Mar 01 00:00:00 EST 2023

Rgl-exomiR-7972, a novel plant exosomal microRNA derived from fresh Rehmanniae Radix, ameliorated lipopolysaccharide-induced acute lung injury and gut dysbiosis.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Qiu FS,Wang JF,Guo MY,Li XJ,Shi CY,Wu F,Zhang HH,Ying HZ,Yu CH

Fri Sep 01 00:00:00 EDT 2023

BRD4 promotes the migration and invasion of bladder cancer cells through the Sonic hedgehog signaling pathway and enhances cisplatin resistance.

Biochemistry and cell biology = Biochimie et biologie cellulaire

Liu T,Zhang Z,Wang C,Huang H,Li Y

Fri Apr 01 00:00:00 EDT 2022

The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling.

Cell reports

Schembs L,Willems A,Hasenpusch-Theil K,Cooper JD,Whiting K,Burr K,Bøstrand SMK,Selvaraj BT,Chandran S,Theil T

Tue May 17 00:00:00 EDT 2022

Cepharanthine hydrochloride inhibits the Wnt/β‑catenin/Hedgehog signaling axis in liver cancer.

Oncology reports

Su GF,Huang ZX,Huang DL,Chen PX,Wang Y,Wang YF

Fri Apr 01 00:00:00 EDT 2022

METTL3-mediated m6A modification of STEAP2 mRNA inhibits papillary thyroid cancer progress by blocking the Hedgehog signaling pathway and epithelial-to-mesenchymal transition.

Cell death & disease

Zhu Y,Peng X,Zhou Q,Tan L,Zhang C,Lin S,Long M

Mon Apr 18 00:00:00 EDT 2022

METTL3-mediated m6A modification of STEAP2 mRNA inhibits papillary thyroid cancer progress by blocking the Hedgehog signaling pathway and epithelial-to-mesenchymal transition.

Cell death & disease

Zhu Y,Peng X,Zhou Q,Tan L,Zhang C,Lin S,Long M

Mon Apr 18 00:00:00 EDT 2022

Involvement of hedgehog signaling in all-trans retinoic acid-mediated suppression of colon cancer.

American journal of translational research

Xu Y,Sun H

Wed Oct 19 00:00:00 EDT 2022

Angiomotin isoform 2 promotes binding of PALS1 to KIF13B at primary cilia and regulates ciliary length and signaling.

Journal of cell science

Morthorst SK,Nielsen C,Farinelli P,Anvarian Z,Rasmussen CBR,Serra-Marques A,Grigoriev I,Altelaar M,Fürstenberg N,Ludwig A,Akhmanova A,Christensen ST,Pedersen LB

Wed Jun 15 00:00:00 EDT 2022

Sterol and oxysterol synthases near the ciliary base activate the Hedgehog pathway.

The Journal of cell biology

Findakly S,Daggubati V,Garcia G,LaStella SA,Choudhury A,Tran C,Li A,Tong P,Garcia JQ,Puri N,Reiter JF,Xu L,Raleigh DR

Mon Jan 04 00:00:00 EST 2021

Epigallocatechin-3-gallate inhibits proliferation and triggers apoptosis in colon cancer via the hedgehog/phosphoinositide 3-kinase pathways.

Canadian journal of physiology and pharmacology

Ding F,Yang S

Wed Sep 01 00:00:00 EDT 2021

Activation of the Hedgehog and Wnt/β-Catenin Signaling Pathways in Basal Cell Carcinoma.

Case reports in dermatology

Takada T

Fri Jan 28 00:00:00 EST 2022

Differential effects of EPA and DHA on DSS-induced colitis in mice and possible mechanisms involved.

Food & function

Zhang Z,Xue Z,Yang H,Zhao F,Liu C,Chen J,Lu S,Zou Z,Zhou Y,Zhang X

Mon Mar 01 00:00:00 EST 2021

Apatinib Suppresses Gastric Cancer Stem Cells Properties by Inhibiting the Sonic Hedgehog Pathway.

Frontiers in cell and developmental biology

Cao W,Li Y,Sun H,Yang C,Zhu J,Xie C,Li X,Wu J,Geng S,Wang L,Sun L,Geng G,Han H,Zhong C

Fri Aug 06 00:00:00 EDT 2021

GLI2 but not GLI1/GLI3 plays a central role in the induction of malignant phenotype of gallbladder cancer.

Oncology reports

Ichimiya S,Onishi H,Nagao S,Koga S,Sakihama K,Nakayama K,Fujimura A,Oyama Y,Imaizumi A,Oda Y,Nakamura M

Mon Mar 01 00:00:00 EST 2021

Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.

Molecular cancer

Gu Y,Wang Y,He L,Zhang J,Zhu X,Liu N,Wang J,Lu T,He L,Tian Y,Fan Z

Thu Oct 14 00:00:00 EDT 2021

MicroRNA miR-19b-3p mediated G protein γ subunit 7 (GNG7) loss contributes lung adenocarcinoma progression through activating Hedgehog signaling.

Bioengineered

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American journal of cancer research

Sun J,Lin W,Li C,Ueki H,Xue R,Sadahira T,Hu H,Wada K,Li N,Liu C,Araki M,Xu A,Huang P

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International journal of clinical and experimental pathology

Sun X,Song J,Li E,Geng H,Li Y,Yu D,Zhong C

Wed Jul 15 00:00:00 EDT 2020

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Cancer medicine

Zhao G,Li H,Guo Q,Zhou A,Wang X,Li P,Zhang S

Wed Apr 01 00:00:00 EDT 2020

Meningioma cells express primary cilia but do not transduce ciliary Hedgehog signals.

Acta neuropathologica communications

Findakly S,Choudhury A,Daggubati V,Pekmezci M,Lang UE,Raleigh DR

Mon Jul 20 00:00:00 EDT 2020

Primary cilium remodeling mediates a cell signaling switch in differentiating neurons.

Science advances

Toro-Tapia G,Das RM

Fri May 01 00:00:00 EDT 2020

Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway.

International journal of food sciences and nutrition

Ge M,Zhang L,Cao L,Xie C,Li X,Li Y,Meng Y,Chen Y,Wang X,Chen J,Zhang Q,Shao J,Zhong C

Thu Aug 01 00:00:00 EDT 2019

Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway.

International journal of food sciences and nutrition

Ge M,Zhang L,Cao L,Xie C,Li X,Li Y,Meng Y,Chen Y,Wang X,Chen J,Zhang Q,Shao J,Zhong C

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Phytotherapy research : PTR

Zhang Q,Cao WS,Wang XQ,Zhang M,Lu XM,Chen JQ,Chen Y,Ge MM,Zhong CY,Han HY

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TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Nature communications

Zhu R,Gires O,Zhu L,Liu J,Li J,Yang H,Ju G,Huang J,Ge W,Chen Y,Lu Z,Wang H

Fri Jun 28 00:00:00 EDT 2019

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Nature communications

Zhu R,Gires O,Zhu L,Liu J,Li J,Yang H,Ju G,Huang J,Ge W,Chen Y,Lu Z,Wang H

Fri Jun 28 00:00:00 EDT 2019

Salinomycin suppresses cancer cell stemness and attenuates TGF-β-induced epithelial-mesenchymal transition of renal cell carcinoma cells.

Chemico-biological interactions

Liu L,Wang Q,Mao J,Qin T,Sun Y,Yang J,Han Y,Li L,Li Q

Tue Dec 25 00:00:00 EST 2018

Salinomycin suppresses cancer cell stemness and attenuates TGF-β-induced epithelial-mesenchymal transition of renal cell carcinoma cells.

Chemico-biological interactions

Liu L,Wang Q,Mao J,Qin T,Sun Y,Yang J,Han Y,Li L,Li Q

Tue Dec 25 00:00:00 EST 2018

Effects of baicalein on pancreatic cancer stem cells via modulation of sonic Hedgehog pathway.

Acta biochimica et biophysica Sinica

Song L,Chen X,Wang P,Gao S,Qu C,Liu L

Fri Jun 01 00:00:00 EDT 2018

Effects of baicalein on pancreatic cancer stem cells via modulation of sonic Hedgehog pathway.

Acta biochimica et biophysica Sinica

Song L,Chen X,Wang P,Gao S,Qu C,Liu L

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Curcumin Suppresses Lung Cancer Stem Cells via Inhibiting Wnt/β-catenin and Sonic Hedgehog Pathways.

Phytotherapy research : PTR

Zhu JY,Yang X,Chen Y,Jiang Y,Wang SJ,Li Y,Wang XQ,Meng Y,Zhu MM,Ma X,Huang C,Wu R,Xie CF,Li XT,Geng SS,Wu JS,Zhong CY,Han HY

Sat Apr 01 00:00:00 EDT 2017

Curcumin Suppresses Lung Cancer Stem Cells via Inhibiting Wnt/β-catenin and Sonic Hedgehog Pathways.

Phytotherapy research : PTR

Zhu JY,Yang X,Chen Y,Jiang Y,Wang SJ,Li Y,Wang XQ,Meng Y,Zhu MM,Ma X,Huang C,Wu R,Xie CF,Li XT,Geng SS,Wu JS,Zhong CY,Han HY

Sat Apr 01 00:00:00 EDT 2017

Curcumin inhibits bladder cancer stem cells by suppressing Sonic Hedgehog pathway.

Biochemical and biophysical research communications

Wang D,Kong X,Li Y,Qian W,Ma J,Wang D,Yu D,Zhong C

Sat Nov 04 00:00:00 EDT 2017

Curcumin inhibits bladder cancer stem cells by suppressing Sonic Hedgehog pathway.

Biochemical and biophysical research communications

Wang D,Kong X,Li Y,Qian W,Ma J,Wang D,Yu D,Zhong C

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Bioinformatics

Protein Aliases: bent body; frizzled family member 11; HGNC:11119; Protein Gx; Protein SMOH; Protein smoothened; seven transmembrane helix receptor; smoothened homolog; smoothened, frizzled family receptor; smoothened, seven transmembrane spanning receptor

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Gene Aliases: bnb; E130215L21Rik; FZD11; Gx; SMO; SMOH; smoothened

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UniProt ID: (Human) Q99835, (Mouse) P56726, (Rat) P97698

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Entrez Gene ID: (Human) 6608, (Mouse) 319757, (Rat) 25273

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Function(s)

G-protein coupled receptor activity patched binding protein binding drug binding Wnt-protein binding Wnt-activated receptor activity signal transducer activity transmembrane signaling receptor activity molecular_function transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SMO Polyclonal Antibody

SMO Antibody (20787-1-AP) in IHC (P)

Product Details

20787-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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