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SMO Antibody (PA5-76145) in WB
Western blot analysis of SMO in extracts of various cell lines. Samples were incubated in SMO polyclonal antibody (Product # PA5-76145) using a dilution of 1:500 followed by HRP Goat Anti-Rabbit IgG secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.
Product Details
PA5-76145
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein of human SMO.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2719872
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
Smoothened drosophila homolog (SMOH) is a serpentine protein belonging to the G-protein coupled receptor Fz/Smo family with 787 aa, seven transmembrane G protein-coupled receptors with 4 glycosylation sites and a putative extracellular amino terminus. Smoothened activated by b-Arrestin 2 and GRK2 acts as a receptor for Shh, the Sonic hedgehog gene product, and is a positive regulator of hedgehog signaling pathway along with the smoothened inhibitor patched protein (PTCH) and regulates vertebrate developmental processes, cellular proliferation and differentiation by activating transcription factor Gli. Smoothened mutants are known to cause sporadic basal cell carcinoma of the skin. It is known to express widely in heart and brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: bent body; frizzled family member 11; HGNC:11119; Protein Gx; Protein SMOH; Protein smoothened; seven transmembrane helix receptor; smoothened homolog; smoothened, frizzled family receptor; smoothened, seven transmembrane spanning receptor
Gene Aliases: bnb; E130215L21Rik; FZD11; Gx; SMO; SMOH; smoothened
UniProt ID: (Human) Q99835, (Mouse) P56726
Entrez Gene ID: (Human) 6608, (Mouse) 319757
negative regulation of transcription from RNA polymerase II promoter
vasculogenesis
osteoblast differentiation
in utero embryonic development
cell fate specification
neural crest cell migration
heart looping
positive regulation of neuroblast proliferation
positive regulation of mesenchymal cell proliferation
determination of left/right asymmetry in lateral mesoderm
type B pancreatic cell development
G-protein coupled receptor signaling pathway
smoothened signaling pathway
positive regulation of hh target transcription factor activity
ventral midline determination
midgut development
anterior/posterior pattern specification
positive regulation of gene expression
negative regulation of gene expression
dentate gyrus development
cerebellar cortex morphogenesis
thalamus development
dorsal/ventral neural tube patterning
smoothened signaling pathway involved in ventral spinal cord patterning
smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation
central nervous system neuron differentiation
cerebral cortex development
negative regulation of epithelial cell differentiation
hair follicle morphogenesis
protein localization to nucleus
multicellular organism growth
non-canonical Wnt signaling pathway
positive regulation of multicellular organism growth
positive regulation of protein import into nucleus
odontogenesis of dentin-containing tooth
negative regulation of apoptotic process
negative regulation of DNA binding
positive regulation of smoothened signaling pathway
positive regulation of transcription from RNA polymerase II promoter
positive regulation of organ growth
astrocyte activation
skeletal muscle fiber development
forebrain morphogenesis
homeostasis of number of cells within a tissue
positive regulation of epithelial cell proliferation
protein stabilization
myoblast migration
negative regulation of hair follicle development
canonical Wnt signaling pathway
detection of cell density by contact stimulus involved in contact inhibition
atrial septum morphogenesis
mammary gland epithelial cell differentiation
epithelial-mesenchymal cell signaling
somite development
pancreas morphogenesis
left/right axis specification
cellular response to cholesterol
renal system development
mesenchymal to epithelial transition involved in metanephric renal vesicle formation
positive regulation of branching involved in ureteric bud morphogenesis
regulation of stem cell population maintenance
regulation of heart morphogenesis
ossification
heart morphogenesis
signal transduction
cell surface receptor signaling pathway
multicellular organism development
determination of left/right symmetry
pattern specification process
central nervous system development
positive regulation of cell proliferation
dorsal/ventral pattern formation
regulation of gene expression
cell development
digestive tract development
embryonic organ development
developmental growth
axon extension involved in axon guidance
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