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SNAIL Antibody (PA5-115940) in ICC/IF
Immunocytochemistry analysis of SNAIL in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115940). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-115940
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SNAI1(Accession O95863), corresponding to amino acid residues N222-R264.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900574
Product Specific Information
Antibody detects endogenous levels of total SNAIL.
Target Information
Zinc finger protein SNAI1 (SNAIL)is a zinc finger transcriptional repressor which down regulates the expression of the ectodermal gene within the mesoderm and functions to form the mesoderm and neural crest. It is reported that SNAI1 acts as an important factor of tumor invasion as evidenced by its role in E-cadherin down-regulation and induction of epithelial-mesenchymal transition (EMT). In human breast cancer, the expression of SNAI1 and/or the homologous SNAI2 (Slug) has been associated with E-cadherin repression, local or distant metastasis, tumor recurrence, or poor prognosis in different tumor series. It is also reported that Snail 1 protein in the stromamay be anew putative prognosis marker for colon tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein sna; Protein snail homolog 1; Sna protein; snai; snail 1; snail 1 homolog; snail 1 zinc finger protein; snail 1, zinc finger protein; snail family zinc finger 1; snail homolog 1; Zinc finger protein SNAI1
Gene Aliases: AI194338; dJ710H13.1; SLUGH2; SNA; Sna1; SNAH; SNAI1; SNAIL; SNAIL1
UniProt ID: (Human) O95863, (Mouse) Q02085
Entrez Gene ID: (Human) 6615, (Mouse) 20613, (Rat) 116490
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein binding
kinase binding
metal ion binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
nucleic acid binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
mesoderm formation
epithelial to mesenchymal transition
epithelial to mesenchymal transition involved in endocardial cushion formation
positive regulation of epithelial to mesenchymal transition
negative regulation of vitamin D biosynthetic process
cell migration
positive regulation of cell migration
hair follicle morphogenesis
negative regulation of DNA damage response, signal transduction by p53 class mediator
positive regulation of transcription, DNA-templated
palate development
cartilage morphogenesis
trophoblast giant cell differentiation
negative regulation of cell differentiation involved in embryonic placenta development
left/right pattern formation
Notch signaling involved in heart development
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
regulation of bicellular tight junction assembly
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
multicellular organism development
mesoderm development
negative regulation of transcription, DNA-templated
negative regulation of cell cycle
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