Invitrogen

SNAIL Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

2 References

View all (36) SNAIL antibodies

Datasheet

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Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

SNAIL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 5

SNAIL Antibody (PA5-23482) in IHC (P)

Immunohistochemical analysis of SNAIL in formalin-fixed paraffin-embedded tissue section of mouse placenta. Samples were incubated in SNAIL polyclonal antibody (Product # PA5-23482) using a dilution of 1:200 followed by HRP labeled anti-rabbit secondary antibody and DAB reagent. Nuclei of cells were counter-stained with hematoxylin.

SNAIL Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Product Image Gallery

SNAIL Antibody — Figure 6. The NFKB-SNAI1 pathway mediates the autophagy-enhanced migration of cancer cells. (a) HepG2 cells were stimulated with CCM or TCM for 20 h. The expression levels of SNAI1, SNAI2, TWIST1, and TWIST2 were measured by western blotting. (b-d) HepG2 cells were transfected with shNC, sh ATG5 , or sh ATG7 lentiviral vectors and then treated with CCM or TCM for 20 h (b), 30 min (d), or other time intervals (c). The levels of SNAI1, ATG5, ATG7, p-RELA, RELA, p-AKT, AKT, p-MAPK14, MAPK14, p-MAPK1/3, MAPK1/3, p-MAPK8/9, and MAPK8/9 were determined by western blotting (b and c). Translocation of the RELA protein was analyzed by confocal microscopy (n = 5) (d). (e) HepG2 cells were transfected with control, si- RELA , or si- SNAI1 RNAs before being exposed to CCM or TCM for 20 h, and then their migration abilities were analyzed (n = 6). (f) Sections of hepatoma samples were double stained with anti-human LC3B (green) and anti-human SNAI1 (red) Abs or anti-human LC3B (green) and anti-human RELA (red) Abs. The levels of SNAI1 and nuclear-located RELA expression at the invading edge of human HCCs with high or low LC3B expression were determined by confocal microscopy. Blue, DAPI; n = 5. One out of 5 representative graphs is shown in A-F. The results shown in D, E and F are expressed as the means +- SEM. ** P < 0.01, *** P < 0.001. {TM}

Product Details

PA5-23482

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A portion of amino acids 1-60 of human SNAI1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2540982

Target Information

Zinc finger protein SNAI1 (SNAIL)is a zinc finger transcriptional repressor which down regulates the expression of the ectodermal gene within the mesoderm and functions to form the mesoderm and neural crest. It is reported that SNAI1 acts as an important factor of tumor invasion as evidenced by its role in E-cadherin down-regulation and induction of epithelial-mesenchymal transition (EMT). In human breast cancer, the expression of SNAI1 and/or the homologous SNAI2 (Slug) has been associated with E-cadherin repression, local or distant metastasis, tumor recurrence, or poor prognosis in different tumor series. It is also reported that Snail 1 protein in the stromamay be anew putative prognosis marker for colon tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Peritumoral monocytes induce cancer cell autophagy to facilitate the progression of human hepatocellular carcinoma.

Autophagy

Chen DP,Ning WR,Li XF,Wei Y,Lao XM,Wang JC,Wu Y,Zheng L

PA5-23482 was used in Immunohistochemistry-immunofluorescence to indicate that cancer cell autophagy is regulated by a collaborative interaction between tumor and immune cell components in distinct HCC microenvironments, thus allowing the inflammatory monocytes to be rerouted in a tumor-promoting direction.

Fri Oct 11 00:00:00 EDT 2019

Peritumoral monocytes induce cancer cell autophagy to facilitate the progression of human hepatocellular carcinoma.

Autophagy

Chen DP,Ning WR,Li XF,Wei Y,Lao XM,Wang JC,Wu Y,Zheng L

Fri Oct 11 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: Protein sna; Protein snail homolog 1; Sna protein; snai; snail 1; snail 1 homolog; snail 1 zinc finger protein; snail 1, zinc finger protein; snail family zinc finger 1; snail homolog 1; Zinc finger protein SNAI1

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Gene Aliases: AI194338; dJ710H13.1; SLUGH2; SNA; Sna1; SNAH; SNAI1; SNAIL; SNAIL1

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UniProt ID: (Human) O95863, (Mouse) Q02085

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Entrez Gene ID: (Human) 6615, (Mouse) 20613

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding kinase binding metal ion binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding nucleic acid binding DNA binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding C2H2 zinc finger transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation mesoderm formation epithelial to mesenchymal transition epithelial to mesenchymal transition involved in endocardial cushion formation positive regulation of epithelial to mesenchymal transition negative regulation of vitamin D biosynthetic process cell migration positive regulation of cell migration hair follicle morphogenesis negative regulation of DNA damage response, signal transduction by p53 class mediator positive regulation of transcription, DNA-templated palate development cartilage morphogenesis trophoblast giant cell differentiation negative regulation of cell differentiation involved in embryonic placenta development left/right pattern formation Notch signaling involved in heart development negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage regulation of bicellular tight junction assembly regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development mesoderm development negative regulation of transcription, DNA-templated

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SNAIL Polyclonal Antibody

SNAIL Antibody (PA5-23482) in IHC (P)

Product Details

PA5-23482

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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