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Invitrogen
SOD1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
SOD1 Antibody (PA1-18275) in ICC/IF
Immunofluorescence analysis of SOD1 was performed using 70% confluent log phase PC-12 cells treated with 50 µM of Hydrogen peroxide for 6 hours. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SOD1 Polyclonal Antibody (Product # PA1-18275) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary ... View More
Product Details
PA1-18275
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues A(24) S G E P V V L S G Q I T(36) of mouse superoxide dismutase (SOD1).
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1087610
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Target Information
SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amyotrophic lateral sclerosis 1 (ALS1); Cu(2+)-Zn2+ superoxide dismutase; Cu/Zn SOD; HGNC:11179; Indophenoloxidase A (IPOA); superoxide dismutase; Superoxide Dismutase 1 (SOD1); Superoxide dismutase [Cu-Zn]
Gene Aliases: B430204E11Rik; Cu/Zn-SOD; CuZnSOD; Ipo-1; Ipo1; Sod-1; Sod1; SODC
UniProt ID: (Mouse) P08228, (Rat) P07632
Entrez Gene ID: (Mouse) 20655, (Rat) 24786
activation of MAPK activity
response to reactive oxygen species
response to superoxide
ovarian follicle development
positive regulation of cytokine production
retina homeostasis
myeloid cell homeostasis
glutathione metabolic process
superoxide metabolic process
cellular iron ion homeostasis
response to oxidative stress
spermatogenesis
embryo implantation
aging
cell aging
sensory perception of sound
locomotory behavior
anterograde axonal transport
retrograde axonal transport
regulation of blood pressure
response to heat
response to organic substance
transmission of nerve impulse
removal of superoxide radicals
response to nutrient levels
peripheral nervous system myelin maintenance
positive regulation of superoxide anion generation
regulation of multicellular organism growth
response to drug
response to hydrogen peroxide
superoxide anion generation
negative regulation of apoptotic process
positive regulation of catalytic activity
regulation of GTPase activity
negative regulation of neuron apoptotic process
response to ethanol
negative regulation of cholesterol biosynthetic process
regulation of protein kinase activity
response to copper ion
muscle cell cellular homeostasis
response to axon injury
hydrogen peroxide biosynthetic process
regulation of mitochondrial membrane potential
oxidation-reduction process
heart contraction
neurofilament cytoskeleton organization
relaxation of vascular smooth muscle
auditory receptor cell stereocilium organization
reactive oxygen species metabolic process
positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
response to amphetamine
double-strand break repair
apoptotic DNA fragmentation
response to carbon monoxide
cellular response to oxidative stress
cellular response to potassium ion
response to antibiotic
cellular response to cadmium ion
cellular response to ATP
response to antipsychotic drug
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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