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Invitrogen
SOD1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
SOD1 Antibody (PA1-30195) in ICC/IF
Immunofluorescence analysis of SOD1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SOD1 Polyclonal Antibody (Product # PA1-30195) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:2... View More
Product Details
PA1-30195
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Hamster,
Human,
Mouse,
Non-human primate,
Sheep,
Pig,
Rabbit,
Rat,
Xenopus
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length protein (Rat).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M sodium phosphate, pH 7.0, with 50% glycerol
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2193755
Product Specific Information
Recommended positive controls: HeLa cell lysate.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Amyotrophic lateral sclerosis 1 (ALS1); Cu(2+)-Zn2+ superoxide dismutase; Cu-Zn superoxide dismutase; Cu-Zn superoxide dismutase C-terminal fragment (150AA); Cu-Zn-superoxide dismutase; Cu/Zn SOD; Cu/Zn superoxide dismutase; CuZn superoxide dismutase; epididymis secretory protein Li 44; HGNC:11179; hSod1; indophenoloxidase A; Indophenoloxidase A (IPOA); SOD, soluble; superoxide dismutase; Superoxide dismutase 1; Superoxide Dismutase 1 (SOD1); superoxide dismutase 1 soluble; superoxide dismutase 1, soluble; superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)); superoxide dismutase 1, soluble S homeolog; Superoxide dismutase [Cu-Zn]; superoxide dismutase, cystolic
Gene Aliases: ALS; ALS1; B430204E11Rik; Cu/Zn-SOD; CuZnSOD; HEL-S-44; homodimer; hSod1; Ipo-1; Ipo1; IPOA; SOD; Sod-1; SOD1; sod1-a; sod1-b; SOD1L1; SODC; XELAEV_18014289mg; xsodb
UniProt ID: (Pig) P04178, (Bovine) P00442, (Human) P00441, (Rabbit) P09212, (Dog) Q8WNN6, (Mouse) P08228, (Rat) P07632
superoxide dismutase activity
ubiquitin-protein transferase activity
copper ion binding
protein binding
zinc ion binding
protein phosphatase 2B binding
protein homodimerization activity
chaperone binding
identical protein binding
Rac GTPase binding
antioxidant activity
oxidoreductase activity
enzyme binding
metal ion binding
oxidoreductase
activation of MAPK activity
protein polyubiquitination
response to superoxide
ovarian follicle development
regulation of cytokine production
positive regulation of cytokine production
retina homeostasis
myeloid cell homeostasis
glutathione metabolic process
superoxide metabolic process
cellular iron ion homeostasis
spermatogenesis
embryo implantation
cell aging
sensory perception of sound
locomotory behavior
regulation of blood pressure
response to heat
response to organic substance
transmission of nerve impulse
removal of superoxide radicals
peripheral nervous system myelin maintenance
regulation of multicellular organism growth
response to drug
response to hydrogen peroxide
positive regulation of catalytic activity
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of neuron apoptotic process
interspecies interaction between organisms
response to ethanol
negative regulation of cholesterol biosynthetic process
regulation of protein kinase activity
muscle cell cellular homeostasis
response to axon injury
hydrogen peroxide biosynthetic process
positive regulation of nitric-oxide synthase biosynthetic process
regulation of mitochondrial membrane potential
oxidation-reduction process
heart contraction
neurofilament cytoskeleton organization
relaxation of vascular smooth muscle
auditory receptor cell stereocilium organization
reactive oxygen species metabolic process
response to reactive oxygen species
placenta development
response to amphetamine
platelet degranulation
anterograde axonal transport
retrograde axonal transport
response to nutrient levels
positive regulation of superoxide anion generation
regulation of T cell differentiation in thymus
response to carbon monoxide
cellular response to potassium ion
superoxide anion generation
positive regulation of apoptotic process
regulation of GTPase activity
regulation of organ growth
response to antibiotic
response to copper ion
thymus development
cellular response to cadmium ion
cellular response to ATP
response to antipsychotic drug
positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
response to oxidative stress
aging
negative regulation of apoptotic process
double-strand break repair
apoptotic DNA fragmentation
cellular response to oxidative stress
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