Invitrogen

SOD1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure
1 Reference
View all (78) SOD1 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-85095

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:10,000
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
- -

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

ELISA (ELISA)

1:1,000-1:10,000
-
Product Specifications

Species Reactivity

Avian, Human, Mouse, Reptile, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOD1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2792243

Product Specific Information

Antigen Retrieval (IF): citrate buffer, pH 6.0, 15 min, and IHC: EDTA based, pH 8.0 buffer, 15min. Keep as concentrated solution.

Predicted reactivity: Mouse (81%), Rat (81%), Pig (80%), Rabbit (82%), Rhesus Monkey (91%), Chimpanzee (100%).

Positive Control: 293T, HeLa, NT2D1, SK-N-AS, IMR32, SK-N-SH, U87-MG, mouse brain, Rat brain, F79, F123.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Amyotrophic lateral sclerosis 1 (ALS1); Cu(2+)-Zn2+ superoxide dismutase; Cu/Zn SOD; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HGNC:11179; hSod1; indophenoloxidase A; Indophenoloxidase A (IPOA); SOD, soluble; superoxide dismutase; Superoxide dismutase 1; Superoxide Dismutase 1 (SOD1); Superoxide dismutase [Cu-Zn]; superoxide dismutase, cystolic

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Gene Aliases: ALS; ALS1; B430204E11Rik; Cu/Zn-SOD; CuZnSOD; HEL-S-44; homodimer; hSod1; Ipo-1; Ipo1; IPOA; SOD; Sod-1; SOD1; SODC

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UniProt ID: (Human) P00441, (Mouse) P08228, (Rat) P07632

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Entrez Gene ID: (Human) 6647, (Mouse) 20655, (Rat) 24786

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Function(s)
superoxide dismutase activity copper ion binding protein binding zinc ion binding protein phosphatase 2B binding identical protein binding protein homodimerization activity Rac GTPase binding chaperone binding antioxidant activity oxidoreductase activity enzyme binding metal ion binding oxidoreductase
Process(es)
activation of MAPK activity response to reactive oxygen species response to superoxide ovarian follicle development positive regulation of cytokine production placenta development retina homeostasis response to amphetamine myeloid cell homeostasis platelet degranulation glutathione metabolic process superoxide metabolic process cellular iron ion homeostasis spermatogenesis embryo implantation cell aging sensory perception of sound locomotory behavior anterograde axonal transport retrograde axonal transport regulation of blood pressure response to heat response to organic substance transmission of nerve impulse removal of superoxide radicals response to nutrient levels peripheral nervous system myelin maintenance positive regulation of superoxide anion generation regulation of T cell differentiation in thymus response to carbon monoxide cellular response to potassium ion regulation of multicellular organism growth response to drug response to hydrogen peroxide superoxide anion generation positive regulation of apoptotic process positive regulation of catalytic activity regulation of GTPase activity negative regulation of neuron apoptotic process response to ethanol negative regulation of cholesterol biosynthetic process regulation of protein kinase activity regulation of organ growth response to antibiotic response to copper ion muscle cell cellular homeostasis thymus development response to axon injury hydrogen peroxide biosynthetic process regulation of mitochondrial membrane potential oxidation-reduction process heart contraction neurofilament cytoskeleton organization relaxation of vascular smooth muscle auditory receptor cell stereocilium organization cellular response to cadmium ion cellular response to ATP reactive oxygen species metabolic process response to antipsychotic drug positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway response to oxidative stress aging negative regulation of apoptotic process double-strand break repair apoptotic DNA fragmentation cellular response to oxidative stress
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