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SOD1 Antibody (MA5-29576) in ICC/IF
Immunofluorescence staining of Human SOD1 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with SOD1 Recombinant Rabbit Monoclonal Antibody (101) (Product # MA5-29576, 1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Product Details
MA5-29576
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
101
Immunogen
Recombinant Human SOD1 protein (Ala2-Gln154)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785418
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human Superoxide Dismutase.
Target Information
SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amyotrophic lateral sclerosis 1 (ALS1); Cu/Zn SOD; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HGNC:11179; hSod1; indophenoloxidase A; Indophenoloxidase A (IPOA); SOD, soluble; superoxide dismutase; Superoxide dismutase 1; Superoxide Dismutase 1 (SOD1); Superoxide dismutase [Cu-Zn]; superoxide dismutase, cystolic
Gene Aliases: ALS; ALS1; HEL-S-44; homodimer; hSod1; IPOA; SOD; SOD1
UniProt ID: (Human) P00441
Entrez Gene ID: (Human) 6647
activation of MAPK activity
response to reactive oxygen species
response to superoxide
ovarian follicle development
positive regulation of cytokine production
placenta development
retina homeostasis
response to amphetamine
myeloid cell homeostasis
platelet degranulation
glutathione metabolic process
superoxide metabolic process
cellular iron ion homeostasis
spermatogenesis
embryo implantation
cell aging
sensory perception of sound
locomotory behavior
anterograde axonal transport
retrograde axonal transport
regulation of blood pressure
response to heat
response to organic substance
transmission of nerve impulse
removal of superoxide radicals
response to nutrient levels
peripheral nervous system myelin maintenance
positive regulation of superoxide anion generation
regulation of T cell differentiation in thymus
response to carbon monoxide
cellular response to potassium ion
regulation of multicellular organism growth
response to drug
response to hydrogen peroxide
superoxide anion generation
positive regulation of apoptotic process
positive regulation of catalytic activity
regulation of GTPase activity
negative regulation of neuron apoptotic process
response to ethanol
negative regulation of cholesterol biosynthetic process
regulation of protein kinase activity
regulation of organ growth
response to antibiotic
response to copper ion
muscle cell cellular homeostasis
thymus development
response to axon injury
hydrogen peroxide biosynthetic process
regulation of mitochondrial membrane potential
oxidation-reduction process
heart contraction
neurofilament cytoskeleton organization
relaxation of vascular smooth muscle
auditory receptor cell stereocilium organization
cellular response to cadmium ion
cellular response to ATP
reactive oxygen species metabolic process
response to antipsychotic drug
positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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