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Invitrogen
SOD1 Recombinant Rabbit Monoclonal Antibody (11H3L1)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
SOD1 Antibody (702783) in ICC/IF
Knockdown of SOD1 was achieved by transfecting HeLa cells with validated specific siRNA (Silencer® select Product # s451). Immunofluorescence analysis was performed on HeLa cells (untransfected, panel a-d), transfected with SOD1 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labelled with Anti-SOD1 Recombinant Rabbit Monoclonal Antibody (Product # 702783, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate ... View More
Product Details
702783
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
11H3L1
Immunogen
Protein corresponding to human SOD1 (aa1-aa154)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2716893
Product Specific Information
This antibody is predicted to react with Monkey, Mouse, Rat
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Amyotrophic lateral sclerosis 1 (ALS1); Cu/Zn SOD; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HGNC:11179; hSod1; indophenoloxidase A; Indophenoloxidase A (IPOA); SOD, soluble; superoxide dismutase; Superoxide dismutase 1; Superoxide Dismutase 1 (SOD1); Superoxide dismutase [Cu-Zn]; superoxide dismutase, cystolic
Gene Aliases: ALS; ALS1; HEL-S-44; homodimer; hSod1; IPOA; SOD; SOD1
UniProt ID: (Human) P00441
Entrez Gene ID: (Human) 6647
activation of MAPK activity
response to reactive oxygen species
response to superoxide
ovarian follicle development
positive regulation of cytokine production
placenta development
retina homeostasis
response to amphetamine
myeloid cell homeostasis
platelet degranulation
glutathione metabolic process
superoxide metabolic process
cellular iron ion homeostasis
spermatogenesis
embryo implantation
cell aging
sensory perception of sound
locomotory behavior
anterograde axonal transport
retrograde axonal transport
regulation of blood pressure
response to heat
response to organic substance
transmission of nerve impulse
removal of superoxide radicals
response to nutrient levels
peripheral nervous system myelin maintenance
positive regulation of superoxide anion generation
regulation of T cell differentiation in thymus
response to carbon monoxide
cellular response to potassium ion
regulation of multicellular organism growth
response to drug
response to hydrogen peroxide
superoxide anion generation
positive regulation of apoptotic process
positive regulation of catalytic activity
regulation of GTPase activity
negative regulation of neuron apoptotic process
response to ethanol
negative regulation of cholesterol biosynthetic process
regulation of protein kinase activity
regulation of organ growth
response to antibiotic
response to copper ion
muscle cell cellular homeostasis
thymus development
response to axon injury
hydrogen peroxide biosynthetic process
regulation of mitochondrial membrane potential
oxidation-reduction process
heart contraction
neurofilament cytoskeleton organization
relaxation of vascular smooth muscle
auditory receptor cell stereocilium organization
cellular response to cadmium ion
cellular response to ATP
reactive oxygen species metabolic process
response to antipsychotic drug
positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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