Invitrogen

SOD1 Monoclonal Antibody (8B10)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

6 Published Figures

11 References

View all (78) SOD1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

SOD1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 21

SOD1 Antibody (MA1-105) in ICC/IF

Immunofluorescence analysis of SOD1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SOD1 (8B10) Mouse Monoclonal Antibody (Product # MA1-105) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175)... View More

SOD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SOD1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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SOD1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with SOD1 siRNA and decrease in signal intensity was observed in western blot application using Anti-SOD1 Mouse Monoclonal Antibody (Product# MA1105). {KD}

Product Details

MA1-105

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunocytochemistry (ICC/IF)

2 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

8B10

Immunogen

Full length human recombinant protein of human SOD1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536811

Product Specific Information

MA1-105 detects SOD1 in human and mouse samples and has been successfully used in Western blot, immunohistochemistry, immunocytochemistry and immunofluorescence applications.

Target Information

SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1 (SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence.

F1000Research

Ayoubi R,Alshafie W,You Z,Southern K,McPherson PS,Laflamme C

Published figure using SOD1 monoclonal antibody (Product # MA1-105) in Immunoprecipitation

Mon Oct 23 00:00:00 EDT 2023

Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1 (SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence.

F1000Research

Ayoubi R,Alshafie W,You Z,Southern K,McPherson PS,Laflamme C

Published figure using SOD1 monoclonal antibody (Product # MA1-105) in Immunoprecipitation

Mon Oct 23 00:00:00 EDT 2023

Antioxidant effect of grape seed extract corrects experimental autoimmune encephalomyelitis behavioral dysfunctions, demyelination, and glial activation.

Frontiers in immunology

Mabrouk M,El Ayed M,Démosthènes A,Aissouni Y,Aouani E,Daulhac-Terrail L,Mokni M,Bégou M

Published figure using SOD1 monoclonal antibody (Product # MA1-105) in Western Blot

Thu Sep 08 00:00:00 EDT 2022

N-Acetylcysteine Enhances the Recovery of Ischemic Limb in Type-2 Diabetic Mice.

Antioxidants (Basel, Switzerland)

Zhu Q,Liu X,Zhu Q,Liu Z,Yang C,Wu H,Zhang L,Xia X,Wang M,Hao H,Cui Y,Zhang G,Hill MA,Flaker GC,Zhou S,Liu Z

MA1-105 was used in Western Blotting to indicate that NAC treatment enhanced the recovery of blood flow and mechanical function in ischemic limbs in T2D mice in association with improved tissue redox/inflammatory status and insulin resistance.

Tue May 31 00:00:00 EDT 2022

Tempol Preserves Endothelial Progenitor Cells in Male Mice with Ambient Fine Particulate Matter Exposure.

Biomedicines

Liu X,Wang A,Chen Z,Cui Y,Hao H,Domeier TL,Sun Q,Liu Z

MA1-105 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to indicate that particulate matter (PM) exposure-induced reduction of endothelial progenitor cell population in male mice may be due to decreased expression of pulmonary superoxide dismutase 1 in male mice.

Sat Jan 29 00:00:00 EST 2022

Combination of Antioxidant Enzyme Overexpression and N-Acetylcysteine Treatment Enhances the Survival of Bone Marrow Mesenchymal Stromal Cells in Ischemic Limb in Mice With Type 2 Diabetes.

Journal of the American Heart Association

Zhu Q,Hao H,Xu H,Fichman Y,Cui Y,Yang C,Wang M,Mittler R,Hill MA,Cowan PJ,Zhang G,He X,Zhou S,Liu Z

MA1-105 was used in Western Blotting to conclude that simultaneous suppression of reactive oxygen species from transplanted bMSCs and host tissue could additively enhance bMSC survival in diabetic ischemic limb with increased therapeutic efficacy in diabetes.

Tue Oct 05 00:00:00 EDT 2021

Combination of Antioxidant Enzyme Overexpression and N-Acetylcysteine Treatment Enhances the Survival of Bone Marrow Mesenchymal Stromal Cells in Ischemic Limb in Mice With Type 2 Diabetes.

Journal of the American Heart Association

Zhu Q,Hao H,Xu H,Fichman Y,Cui Y,Yang C,Wang M,Mittler R,Hill MA,Cowan PJ,Zhang G,He X,Zhou S,Liu Z

Tue Oct 05 00:00:00 EDT 2021

The copper chaperone CCS facilitates copper binding to MEK1/2 to promote kinase activation.

The Journal of biological chemistry

Grasso M,Bond GJ,Kim YJ,Boyd S,Matson Dzebo M,Valenzuela S,Tsang T,Schibrowsky NA,Alwan KB,Blackburn NJ,Burslem GM,Wittung-Stafshede P,Winkler DD,Marmorstein R,Brady DC

MA1-105 was used in Western Blot to indicate that the Cu chaperone CCS provides fidelity within a complex biological system to achieve appropriate installation of Cu within the MEK1 kinase active site that in turn modulates kinase activity and supports the development of novel MEK1/2 inhibitors that target the Cu structural interface or blunt dedicated Cu delivery mechanisms via CCS.

Wed Dec 01 00:00:00 EST 2021

Fine tuning of the unfolded protein response by ISRIB improves neuronal survival in a model of amyotrophic lateral sclerosis.

Cell death & disease

Bugallo R,Marlin E,Baltanás A,Toledo E,Ferrero R,Vinueza-Gavilanes R,Larrea L,Arrasate M,Aragón T

MA1-105 was used in Immunocytochemistry-immunoflourescence to identify ISRIB-mediated translational reprogramming as a new potential ALS therapy.

Tue May 26 00:00:00 EDT 2020

Fine tuning of the unfolded protein response by ISRIB improves neuronal survival in a model of amyotrophic lateral sclerosis.

Cell death & disease

Bugallo R,Marlin E,Baltanás A,Toledo E,Ferrero R,Vinueza-Gavilanes R,Larrea L,Arrasate M,Aragón T

MA1-105 was used in Immunocytochemistry-immunoflourescence to identify ISRIB-mediated translational reprogramming as a new potential ALS therapy.

Tue May 26 00:00:00 EDT 2020

Concomitant overexpression of triple antioxidant enzymes selectively increases circulating endothelial progenitor cells in mice with limb ischaemia.

Journal of cellular and molecular medicine

Liu L,Cui Y,Li X,Que X,Xiao Y,Yang C,Zhang J,Xie X,Cowan PJ,Tian J,Hao H,Liu Z

MA1-105 was used in Western Blotting to determine if ischaemia induced reactive oxygen species differentially regulate individual endothelial progenitor cell populations.

Sat Jun 01 00:00:00 EDT 2019

Mitochondrial superoxide disrupts the metabolic and epigenetic landscape of CD4⁺ and CD8⁺ T-lymphocytes.

Redox biology

Moshfegh CM,Collins CW,Gunda V,Vasanthakumar A,Cao JZ,Singh PK,Godley LA,Case AJ

Published figure using SOD1 monoclonal antibody (Product # MA1-105) in Western Blot

Tue Oct 01 00:00:00 EDT 2019

Protective Effects of Kuding Tea (Ilex kudingcha C. J. Tseng) Polyphenols on UVB-Induced Skin Aging in SKH1 Hairless Mice.

Molecules (Basel, Switzerland)

Yi R,Zhang J,Sun P,Qian Y,Zhao X

MA1-105 was used in Western Blotting to study the protective effects of Kuding tea polyphenols on ultraviolet B-induced skin injury of SKH1 hairless mice.

Wed Mar 13 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: Amyotrophic lateral sclerosis 1 (ALS1); Cu(2+)-Zn2+ superoxide dismutase; Cu/Zn SOD; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HGNC:11179; hSod1; indophenoloxidase A; Indophenoloxidase A (IPOA); SOD, soluble; superoxide dismutase; Superoxide dismutase 1; Superoxide Dismutase 1 (SOD1); Superoxide dismutase [Cu-Zn]; superoxide dismutase, cystolic

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Gene Aliases: ALS; ALS1; B430204E11Rik; Cu/Zn-SOD; CuZnSOD; HEL-S-44; homodimer; hSod1; Ipo-1; Ipo1; IPOA; SOD; Sod-1; SOD1; SODC

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UniProt ID: (Human) P00441, (Mouse) P08228

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Entrez Gene ID: (Human) 6647, (Mouse) 20655

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Function(s)

superoxide dismutase activity copper ion binding protein binding zinc ion binding protein phosphatase 2B binding identical protein binding protein homodimerization activity Rac GTPase binding chaperone binding antioxidant activity oxidoreductase activity enzyme binding metal ion binding oxidoreductase

Process(es)

activation of MAPK activity response to reactive oxygen species response to superoxide ovarian follicle development positive regulation of cytokine production placenta development retina homeostasis response to amphetamine myeloid cell homeostasis platelet degranulation glutathione metabolic process superoxide metabolic process cellular iron ion homeostasis spermatogenesis embryo implantation cell aging sensory perception of sound locomotory behavior anterograde axonal transport retrograde axonal transport regulation of blood pressure response to heat response to organic substance transmission of nerve impulse removal of superoxide radicals response to nutrient levels peripheral nervous system myelin maintenance positive regulation of superoxide anion generation regulation of T cell differentiation in thymus response to carbon monoxide cellular response to potassium ion regulation of multicellular organism growth response to drug response to hydrogen peroxide superoxide anion generation positive regulation of apoptotic process positive regulation of catalytic activity regulation of GTPase activity negative regulation of neuron apoptotic process response to ethanol negative regulation of cholesterol biosynthetic process regulation of protein kinase activity regulation of organ growth response to antibiotic response to copper ion muscle cell cellular homeostasis thymus development response to axon injury hydrogen peroxide biosynthetic process regulation of mitochondrial membrane potential oxidation-reduction process heart contraction neurofilament cytoskeleton organization relaxation of vascular smooth muscle auditory receptor cell stereocilium organization cellular response to cadmium ion cellular response to ATP reactive oxygen species metabolic process response to antipsychotic drug positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway response to oxidative stress aging negative regulation of apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SOD1 Monoclonal Antibody (8B10)

SOD1 Antibody (MA1-105) in ICC/IF

Product Details

MA1-105

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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