Invitrogen

SOD2 Monoclonal Antibody (1H6)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 References

View all (46) SOD2 (MnSOD) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

SOD2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

SOD2 Antibody (MA1-106) in ICC/IF

Immunofluorescence analysis of SOD2 was performed using 70% confluent log phase A549 cells treated with TNF alpha (20 ng/mL for 72h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SOD2 Polyclonal Antibody (Product # MA1-106) at 2 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluo... View More

SOD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SOD2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

SOD2 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis using SOD2 Polyclonal Antibody (Product # MA1-106) shows increased expression of SOD2 in A549 upon treatment with TNF alpha. {TM}

Product Details

MA1-106

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

2 µg/mL

Immunoprecipitation (IP)

2 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

1H6

Immunogen

Full length recombinant protein of human SOD2

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536812

Product Specific Information

MA1-106 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, immunohistochemistry and immunoprecipitation applications with human samples. This antibody is not suitable for mouse liver tissue in IHC applications and is not suitable for PC12 cells in IF applications.

The MA1-106 immunogen is full length recombinant protein of human SOD2.

Target Information

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD-1), mitochondrial manganese SOD (Mn-SOD/SOD-2) and extracellular Cu, Zn-SOD (EC-SOD/SOD-3). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80 kDa tetrameric enzyme SOD2, is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin-binding multimer of disulfide-linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase (CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The protective effect of fibroblast growth factor-21 in alcoholic cardiomyopathy: a role in protecting cardiac mitochondrial function.

The Journal of pathology

Ferrer-Curriu G,Guitart-Mampel M,Rupérez C,Zamora M,Crispi F,Villarroya F,Fernández-Solà J,Garrabou G,Planavila A

MA1-106 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to conclude that plasma levels and cardiac myocyte fibroblast growth factor 21 expression were induced in response to chronic alcohol consumption.

Mon Feb 01 00:00:00 EST 2021

Impaired flickering of the permeability transition pore causes SPG7 spastic paraplegia.

EBioMedicine

Sambri I,Massa F,Gullo F,Meneghini S,Cassina L,Carraro M,Dina G,Quattrini A,Patanella L,Carissimo A,Iuliano A,Santorelli F,Codazzi F,Grohovaz F,Bernardi P,Becchetti A,Casari G

MA1-106 was used in Western Blotting to demonstrate that paraplegin is required for efficient transient opening of the mPTP, that is impaired in both SPG7 patients-derived fibroblasts and primary neurons from Spg7-/- mice.

Sun Nov 01 00:00:00 EDT 2020

Chloramphenicol Mitigates Oxidative Stress by Inhibiting Translation of Mitochondrial Complex I in Dopaminergic Neurons of Toxin-Induced Parkinson's Disease Model.

Oxidative medicine and cellular longevity

Han J,Kim SJ,Ryu MJ,Jang Y,Lee MJ,Ju X,Lee YL,Cui J,Shong M,Heo JY,Kweon GR

MA1-106 was used in Western Blotting to show chloramphenicol in a toxin-induced Parkinson's Disease model mediates inhibition of mitochondrial complex I in dopaminergic neurons leading to mitigation of oxidative stress.

Fri Mar 20 00:00:00 EDT 2020

GATA-1 isoforms differently contribute to the production and compartmentation of reactive oxygen species in the myeloid leukemia cell line K562.

Journal of cellular physiology

Riccio P,Sessa R,de Nicola S,Petruzziello F,Trombetti S,Menna G,Pepe G,Maddalena P,Izzo P,Grosso M

MA1-106 was used in Western Blotting to study the mechanism of action of GATA-1 isoforms in the regulation of hematopoiesis.

Fri Nov 01 00:00:00 EDT 2019

EAE-induced upregulation of mitochondrial MnSOD is associated with increases of mitochondrial SGK1 and Tom20 protein in the mouse kidney cortex.

The journal of physiological sciences : JPS

Hira S,Packialakshmi B,Zhou X

MA1-106 was used in Western Blotting to elucidate the underlying mechanism by which severe experimental autoimmune encephalomyelitis increases MnSOD protein abundance, in a HEK293 cell model.

Sun Sep 01 00:00:00 EDT 2019

JAK2V617F mediates resistance to DNA damage-induced apoptosis by modulating FOXO3A localization and Bcl-xL deamidation.

Oncogene

Ahn JS,Li J,Chen E,Kent DG,Park HJ,Green AR

MA1-106 was used in western blot to elucidate the mechanism by which NHE-1 expression is inhibited by JAK2V617F

Thu Apr 28 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: indophenoloxidase B; IPO B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; Mn superoxide dismutase; superoxide dismutase; Superoxide dismutase [Mn], mitochondrial

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Gene Aliases: IPO-B; IPOB; Mn-SOD; MNSOD; MVCD6; SOD2

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UniProt ID: (Human) P04179

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Entrez Gene ID: (Human) 6648

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Function(s)

superoxide dismutase activity manganese ion binding identical protein binding oxidoreductase metabolite interconversion enzyme

Process(es)

response to reactive oxygen species response to superoxide age-dependent response to reactive oxygen species release of cytochrome c from mitochondria vasodilation by acetylcholine involved in regulation of systemic arterial blood pressure regulation of transcription from RNA polymerase II promoter superoxide metabolic process regulation of blood pressure negative regulation of cell proliferation removal of superoxide radicals oxygen homeostasis negative regulation of neuron apoptotic process protein homotetramerization oxidation-reduction process negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway

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SOD2 Monoclonal Antibody (1H6)

SOD2 Antibody (MA1-106) in ICC/IF

Product Details

MA1-106

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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