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SOX1 Antibody (MA5-51497) in WB
Western blot analysis of SOX1 in various lysates. Samples were incubated with SOX1 Recombinant monoclonal antibody (Product # MA5-51497) using a dilution of 1:2,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
MA5-51497
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
0H4C1
Immunogen
Recombinant protein of human SOX1.
Conjugate
Form
Liquid
Concentration
0.23 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3093924
Target Information
SOX1, also known as SRY (sex determining region Y)-box 1, is a transcription factor belonging to the SRY-related HMG box family of transcription factors with a HMG box DNA-binding domain. SOX1 works through multiple independent pathways to promote neuronal cell fate determination and differentiation. This nuclear protein acts as a modulator of LINE retroposons promoter activity and is also involved in the development of the central nervous system, mainly neural and lens development. Transcriptional activator SOX1 binds directly to the HES1 promoter and suppresses HES1 transcription, thus attenuating Notch signaling. It also binds to beta-catenin and suppresses beta-catenin-mediated TCF/LEF signaling, thus potentially attenuating the WNT signaling pathway. Expression of SOX1 is seen mostly in embryonic tissues and moderately in brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: SRY (sex determining region Y)-box 1; SRY box 1; SRY-box containing gene 1; SRY-related HMG-box gene 1; Transcription factor SOX-1; transcription factor SOX-1-like
Gene Aliases: BB176347; Sox-1; SOX1
UniProt ID: (Human) O00570, (Mouse) P53783
Entrez Gene ID: (Rat) 100912302, (Human) 6656, (Mouse) 20664
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
sequence-specific DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
HMG box transcription factor
neuron migration
lens morphogenesis in camera-type eye
transcription from RNA polymerase II promoter
ventral spinal cord interneuron specification
forebrain neuron differentiation
forebrain neuron development
forebrain development
positive regulation of transcription from RNA polymerase II promoter
chromatin organization
regulation of transcription, DNA-templated
transcription, DNA-templated
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