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Invitrogen
SOX10 Recombinant Rabbit Monoclonal Antibody (5H7L26)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
SOX10 Antibody (703439) in ICC/IF
Knockdown of SOX10 was achieved by transfecting A-375 cells with specific siRNA (Silencer® select Product # s13309, s13311). Immunofluorescence analysis was performed on A-375 cells (untransfected, panel a-d), transfected with SOX10 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). A-375 cells were fixed, permeabilized, and labelled with Anti-SOX10 Recombinant Rabbit Monoclonal Antibody (Product # 703439, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488... View More
Product Details
703439
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
5H7L26
Immunogen
Peptides corresponding to human SOX10 [1) aa49-aa65 2) aa252-aa269 3) aa177-aa189]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809236
Product Specific Information
This antibody is predicted to react with Monkey, Cat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dominant megacolon; dominant megacolon, mouse, human homolog of; MGC15649; Protein SOX-21; SRY; SRY (sex determining region Y)-box 10; SRY box 10; SRY-box containing gene 10; SRY-related HMG-box gene 10; Transcription factor SOX-10; Transcription factor SOX-M
Gene Aliases: DOM; PCWH; Sox-10; SOX10; Sox21; WS2E; WS4; WS4C
UniProt ID: (Human) P56693, (Mouse) Q04888
Entrez Gene ID: (Human) 6663, (Mouse) 20665
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription factor binding
chromatin binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription coactivator activity
protein binding
transcription factor binding
identical protein binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription regulatory region DNA binding
in utero embryonic development
neural crest cell migration
positive regulation of neuroblast proliferation
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
peripheral nervous system development
positive regulation of gliogenesis
cell differentiation
melanocyte differentiation
lacrimal gland development
negative regulation of apoptotic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cell maturation
enteric nervous system development
digestive tract morphogenesis
developmental growth
oligodendrocyte differentiation
morphogenesis of a branching epithelium
negative regulation of canonical Wnt signaling pathway
regulation of transcription from RNA polymerase II promoter
anatomical structure morphogenesis
stem cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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