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Invitrogen
SOX17 Recombinant Rabbit Monoclonal Antibody (6H42L1)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
SOX17 Antibody (703063) in ICC/IF
For immunofluorescence analysis, iPS cells and iPS cells differentiated to definitive endoderm were fixed and permeabilized for detection of endogenous SOX17 using Anti-SOX17 Recombinant Rabbit Monoclonal Antibody (Product # 703063, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (... View More
Product Details
703063
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
6H42L1
Immunogen
Protein corresponding to human Sox17 [aa177-aa414]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2784556
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Bovine.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
SOX17 (transcription factor SOX-17) belongs to the SRY-related HMB box family of transcription factors. It acts as a transcription regulator that binds target promoter DNA and bends the DNA. It binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'. SOX17 modulates transcriptional regulation via WNT3A and inhibits Wnt signaling. It promotes degradation of activated CTNNB1. SOX17 plays an essential role in the regulation of embryonic development and is required for normal looping of the embryonic hear tube and for normal development of the definitive gut endoderm. Mutations in the gene can result in vesicoureteral reflux.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: SRY (sex determining region Y)-box 17; SRY box 17; SRY-related HMG-box transcription factor SOX17; Transcription factor SOX-17
Gene Aliases: SOX17; VUR3
UniProt ID: (Human) Q9H6I2
Entrez Gene ID: (Human) 64321
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription coactivator activity
protein binding
beta-catenin binding
transcription factor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
protein heterodimerization activity
HMG box transcription factor
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
vasculogenesis
endoderm formation
inner cell mass cellular morphogenesis
heart looping
cardiogenic plate morphogenesis
embryonic heart tube morphogenesis
outflow tract morphogenesis
negative regulation of Wnt signaling pathway involved in heart development
regulation of transcription, DNA-templated
spermatogenesis
endodermal cell fate determination
rostrocaudal neural tube patterning
signal transduction involved in regulation of gene expression
negative regulation of cell growth
protein destabilization
embryonic heart tube development
cell migration involved in gastrulation
negative regulation of mesodermal cell fate specification
mRNA transcription from RNA polymerase II promoter
positive regulation of cell differentiation
positive regulation of protein catabolic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of embryonic development
embryonic foregut morphogenesis
positive regulation of skeletal muscle tissue development
stem cell fate specification
protein stabilization
canonical Wnt signaling pathway
endocardium formation
regulation of transcription from RNA polymerase II promoter involved in definitive endodermal cell fate specification
cardiac cell fate determination
heart formation
endocardial cell differentiation
common bile duct development
gall bladder development
endodermal digestive tract morphogenesis
renal system development
regulation of stem cell proliferation
negative regulation of canonical Wnt signaling pathway
regulation of stem cell division
regulation of cardiac cell fate specification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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