Invitrogen

SOX2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

16 Published Figures

29 References

View all (90) SOX2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

SOX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 19

SOX2 Antibody (PA1-16968) in IHC (P)

Immunohistochemical analysis of SOX2 in human uterus, endometrial glands. Samples were incubated in SOX2 polyclonal antibody (Product # PA1-16968).

SOX2 Antibody in Immunocytochemistry (ICC/IF)

SOX2 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

SOX2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

SOX2 Antibody — Fig. 1 Loss of Oct4 and maintenance of Sox2 expression during retinoic acid-induced differentiation. Oct4 expression begins to decline after 24-hour exposure to retinoic acid (1 µM), with the main transition to an Oct4- state occurring between 24 and 48 h of RA treatment. After 72 h, ~ 70% of the population retain an Oct4-Sox2 + phenotype (a, c). A schematic depicting the change in gene expression in ESC populations towards the predominant progenitor state during two differentiation protocols: LIF-withdrawal in serum-containing media and retinoic acid addition (b). Quantification of flow cytometry data in a demonstrates the maintenance of the Oct4-Sox2 + state between 48 and 72 h of RA treatment (c), n = 9 (three biological replicates, three technical replicates for each biological replicate). Scale bar: 50 µm {TM}

Product Details

PA1-16968

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:125-1:250

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:250

View 16 publications 16 publications

Flow Cytometry (Flow)

1:100

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Sheep, Rat

Published species

Fish, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide made to the N-terminal region of human SOX2 protein (within residues 1-100). [Swiss-Prot# P48431]

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2195781

Product Specific Information

The target sequence has 92% sequence homology with chicken, 86% sequence homology with xenopus, and 71% sequence homology with zebrafish.

Suggested positive control: antigen standard for SOX2 (transient overexpression lysate), mouse brain lysate, mouse brain protein, uterual endometrial glands.

Target Information

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The neonatal southern white rhinoceros ovary contains oogonia in germ cell nests.

Communications biology

Appeltant R,Hermes R,Holtze S,Modina SC,Galli C,Bjarkadottir BD,Adeniran BV,Wei X,Swegen A,Hildebrandt TB,Williams SA

Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Immunohistochemistry (Paraffin)

Tue Oct 17 00:00:00 EDT 2023

Generation of the First Human In Vitro Model for McArdle Disease Based on iPSC Technology.

International journal of molecular sciences

Ortuño-Costela MDC,Cerrada V,Moreno-Izquierdo A,García-Consuegra I,Laberthonnière C,Delourme M,Garesse R,Arenas J,Fuster García C,García García G,Millán JM,Magdinier F,Gallardo ME

PA1-16968 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to test in this model the rescue of PYGM expression by two different read-through compounds (PTC124 and RTC13).

Sat Nov 12 00:00:00 EST 2022

Generation of the First Human In Vitro Model for McArdle Disease Based on iPSC Technology.

International journal of molecular sciences

Ortuño-Costela MDC,Cerrada V,Moreno-Izquierdo A,García-Consuegra I,Laberthonnière C,Delourme M,Garesse R,Arenas J,Fuster García C,García García G,Millán JM,Magdinier F,Gallardo ME

Sat Nov 12 00:00:00 EST 2022

Autophagy Enhances Longevity of Induced Pluripotent Stem Cell-Derived Endothelium via mTOR-Independent ULK1 Kinase.

Stem cells translational medicine

Hekman KE,Koss KM,Ivancic DZ,He C,Wertheim JA

PA1-16968 was used in Immunocytochemistry to find that autophagy activity was stimulated during differentiation but stagnated in mature iPSC-ECs.

Fri Nov 18 00:00:00 EST 2022

Autophagy Enhances Longevity of Induced Pluripotent Stem Cell-Derived Endothelium via mTOR-Independent ULK1 Kinase.

Stem cells translational medicine

Hekman KE,Koss KM,Ivancic DZ,He C,Wertheim JA

PA1-16968 was used in Immunocytochemistry to find that autophagy activity was stimulated during differentiation but stagnated in mature iPSC-ECs.

Fri Nov 18 00:00:00 EST 2022

Aberrant Expression of Circulating MicroRNA Leads to the Dysregulation of Alpha-Synuclein and Other Pathogenic Genes in Parkinson's Disease.

Frontiers in cell and developmental biology

Cai M,Chai S,Xiong T,Wei J,Mao W,Zhu Y,Li X,Wei W,Dai X,Yang B,Liu W,Shu B,Wang M,Lu T,Cai Y,Zheng Z,Mei Z,Zhou Y,Yang J,Zhao J,Shen L,Ho JWK,Chen J,Xiong N

Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Immunocytochemistry

Fri Sep 10 00:00:00 EDT 2021

Cell-type-specific profiling of human cellular models of fragile X syndrome reveal PI3K-dependent defects in translation and neurogenesis.

Cell reports

Raj N,McEachin ZT,Harousseau W,Zhou Y,Zhang F,Merritt-Garza ME,Taliaferro JM,Kalinowska M,Marro SG,Hales CM,Berry-Kravis E,Wolf-Ochoa MW,Martinez-Cerdeño V,Wernig M,Chen L,Klann E,Warren ST,Jin P,Wen Z,Bassell GJ

Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Immunocytochemistry

Tue Apr 13 00:00:00 EDT 2021

Gelatin methacrylate hydrogels culture model for glioblastoma cells enriches for mesenchymal-like state and models interactions with immune cells.

Scientific reports

Shah N,Hallur PM,Ganesh RA,Sonpatki P,Naik D,Chandrachari KP,Puchalski RB,Chaubey A

Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Immunocytochemistry

Mon Sep 06 00:00:00 EDT 2021

Impaired stem cell differentiation and somatic cell reprogramming in DIDO3 mutants with altered RNA processing and increased R-loop levels.

Cell death & disease

Fütterer A,Talavera-Gutiérrez A,Pons T,de Celis J,Gutiérrez J,Domínguez Plaza V,Martínez-A C

PA1-16968 was used in Immunocytochemistry to investigate if DIDO3 has essential functions in embryonic stem cell differentiation and somatic cell reprogramming by supporting accurate RNA metabolism, with its exon16-encoded domain playing the main role.

Mon Jun 21 00:00:00 EDT 2021

Differentiation of Human Mesenchymal Stem Cells from Wharton's Jelly Towards Neural Stem Cells Using A Feasible and Repeatable Protocol.

Cells

Kruminis-Kaszkiel E,Osowski A,Bejer-Oleńska E,Dziekoński M,Wojtkiewicz J

Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Flow Cytometry

Tue Mar 17 00:00:00 EDT 2020

Differentiation of Human Mesenchymal Stem Cells from Wharton's Jelly Towards Neural Stem Cells Using A Feasible and Repeatable Protocol.

Cells

Kruminis-Kaszkiel E,Osowski A,Bejer-Oleńska E,Dziekoński M,Wojtkiewicz J

Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Flow Cytometry

Tue Mar 17 00:00:00 EDT 2020

Differentiation of Human Mesenchymal Stem Cells from Wharton's Jelly Towards Neural Stem Cells Using A Feasible and Repeatable Protocol.

Cells

Kruminis-Kaszkiel E,Osowski A,Bejer-Oleńska E,Dziekoński M,Wojtkiewicz J

Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Flow Cytometry

Tue Mar 17 00:00:00 EDT 2020

miR-590-3p and Its Downstream Target Genes in HCC Cell Lines.

Analytical cellular pathology (Amsterdam)

Elfar M,Amleh A

Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Immunocytochemistry

Fri May 15 00:00:00 EDT 2020

miR-590-3p and Its Downstream Target Genes in HCC Cell Lines.

Analytical cellular pathology (Amsterdam)

Elfar M,Amleh A

Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Immunocytochemistry

Fri May 15 00:00:00 EDT 2020

miR-590-3p and Its Downstream Target Genes in HCC Cell Lines.

Analytical cellular pathology (Amsterdam)

Elfar M,Amleh A

Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Immunocytochemistry

Fri May 15 00:00:00 EDT 2020

Generation and characterization of a human iPSC line (UAMi004-A) from a patient with propionic acidemia due to defects in the PCCB gene.

Stem cell research

López-Márquez A,Alonso-Barroso E,Cerro-Tello G,Bravo-Alonso I,Arribas-Carreira L,Briso-Montiano Á,Navarrete R,Pérez-Cerdá C,Ugarte M,Pérez B,Desviat LR,Richard E

PA1-16968 was used in Immunocytochemistry to conclude that iPSCs have shown full pluripotency, differentiation capacity and genetic stability. The generated iPSC line represents a useful tool to study the pathomechanisms underlying the deficiency.

Mon Jul 01 00:00:00 EDT 2019

Generation of two transgene-free human iPSC lines from CD133⁺ cord blood cells.

Stem cell research

Arellano-Viera E,Zabaleta L,Castaño J,Azkona G,Carvajal-Vergara X,Giorgetti A

PA1-16968 was used in Flow Cytometry, Immunocytochemistry to characterise two human induced pluripotent stem cell lines, PRYDi-CB5 and PRYDi-CB40, isolated from umbilical cord blood of a female child using non-integrative Sendai virus.

Mon Apr 01 00:00:00 EDT 2019

Generation of two transgene-free human iPSC lines from CD133⁺ cord blood cells.

Stem cell research

Arellano-Viera E,Zabaleta L,Castaño J,Azkona G,Carvajal-Vergara X,Giorgetti A

Mon Apr 01 00:00:00 EDT 2019

Altered transcriptional regulatory proteins in glioblastoma and YBX1 as a potential regulator of tumor invasion.

Scientific reports

Gupta MK,Polisetty RV,Sharma R,Ganesh RA,Gowda H,Purohit AK,Ankathi P,Prasad K,Mariswamappa K,Lakshmikantha A,Uppin MS,Sundaram C,Gautam P,Sirdeshmukh R

Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Immunohistochemistry

Mon Jul 29 00:00:00 EDT 2019

Dynamic intercellular transport modulates the spatial patterning of differentiation during early neural commitment.

Nature communications

Glen CM,McDevitt TC,Kemp ML

Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Flow Cytometry

Fri Oct 05 00:00:00 EDT 2018

Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.

Stem cell research & therapy

Ventura Ferreira MS,Bienert M,Müller K,Rath B,Goecke T,Opländer C,Braunschweig T,Mela P,Brümmendorf TH,Beier F,Neuss S

Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Immunofluorescence

Mon Feb 05 00:00:00 EST 2018

Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.

Stem cell research & therapy

Ventura Ferreira MS,Bienert M,Müller K,Rath B,Goecke T,Opländer C,Braunschweig T,Mela P,Brümmendorf TH,Beier F,Neuss S

Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Immunofluorescence

Mon Feb 05 00:00:00 EST 2018

bFGF-mediated pluripotency maintenance in human induced pluripotent stem cells is associated with NRAS-MAPK signaling.

Cell communication and signaling : CCS

Haghighi F,Dahlmann J,Nakhaei-Rad S,Lang A,Kutschka I,Zenker M,Kensah G,Piekorz RP,Ahmadian MR

PA1-16968 was used in Western Blotting to investigate the effect of bFGF and its downstream pathways in pluripotent vs. differentiated human induced (hi) PSCs.

Wed Dec 05 00:00:00 EST 2018

bFGF-mediated pluripotency maintenance in human induced pluripotent stem cells is associated with NRAS-MAPK signaling.

Cell communication and signaling : CCS

Haghighi F,Dahlmann J,Nakhaei-Rad S,Lang A,Kutschka I,Zenker M,Kensah G,Piekorz RP,Ahmadian MR

PA1-16968 was used in Western Blotting to investigate the effect of bFGF and its downstream pathways in pluripotent vs. differentiated human induced (hi) PSCs.

Wed Dec 05 00:00:00 EST 2018

Generation of a human iPSC line, IISHDOi002-A, with a 46, XY/47, XYY mosaicism and belonging to an African mitochondrial haplogroup.

Stem cell research

Ortuño-Costela MDC,Moreno-Izquierdo A,Garesse R,Gallardo ME

PA1-16968 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to generate human iPSC line, IISHDOi002-A, from commercial primary normal human dermal fibroblasts belonging to an African mitochondrial haplogroup (L3), and with a 46, XY/47, XYY mosaicism.

Sun Apr 01 00:00:00 EDT 2018

Generation and characterization of two human iPSC lines from patients with methylmalonic acidemia cblB type.

Stem cell research

Richard E,Brasil S,Briso-Montiano A,Alonso-Barroso E,Gallardo ME,Merinero B,Ugarte M,Desviat LR,Pérez B

PA1-16968 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to generate from fibroblasts of two siblings with methylmalonic acidemia cblB type carrying mutations in the MMAB gene: c.287T➔C (p.Ile96Thr) and a splicing loss-of-function variant c.584G➔A affecting the last nucleotide of exon 7 in MMAB (p.Ser174Cysfs*23).

Tue May 01 00:00:00 EDT 2018

SETD7 Regulates the Differentiation of Human Embryonic Stem Cells.

PloS one

Castaño J,Morera C,Sesé B,Boue S,Bonet-Costa C,Martí M,Roque A,Jordan A,Barrero MJ

PA1-16968 was used in immunohistochemistry to study how differentiation of human embryonic stem cells is regulated by SETD7

Tue Jul 26 00:00:00 EDT 2016

SETD7 Regulates the Differentiation of Human Embryonic Stem Cells.

PloS one

Castaño J,Morera C,Sesé B,Boue S,Bonet-Costa C,Martí M,Roque A,Jordan A,Barrero MJ

PA1-16968 was used in immunohistochemistry to study how differentiation of human embryonic stem cells is regulated by SETD7

Tue Jul 26 00:00:00 EDT 2016

SETD7 Regulates the Differentiation of Human Embryonic Stem Cells.

PloS one

Castaño J,Morera C,Sesé B,Boue S,Bonet-Costa C,Martí M,Roque A,Jordan A,Barrero MJ

PA1-16968 was used in immunohistochemistry to study how differentiation of human embryonic stem cells is regulated by SETD7

Tue Jul 26 00:00:00 EDT 2016

Generation of a human iPSC line from a patient with Leigh syndrome.

Stem cell research

Galera T,Zurita F,González-Páramos C,Moreno-Izquierdo A,Fraga MF,Fernández AF,Garesse R,Gallardo ME

PA1-16968 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to generate a human iPSC line LND554SV.3 from heteroplasmic fibroblasts of a patient with Leigh syndrome carrying a mutation in the MT-ND5 gene (m.13513GNA; p.D393N).

Fri Jan 01 00:00:00 EST 2016

Expansion and differentiation of germline-derived pluripotent stem cells on biomaterials.

Tissue engineering. Part A

Hoss M,Šarić T,Denecke B,Peinkofer G,Bovi M,Groll J,Ko K,Salber J,Halbach M,Schöler HR,Zenke M,Neuss S

Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Immunofluorescence

Wed May 01 00:00:00 EDT 2013

Expansion and differentiation of germline-derived pluripotent stem cells on biomaterials.

Tissue engineering. Part A

Hoss M,Šarić T,Denecke B,Peinkofer G,Bovi M,Groll J,Ko K,Salber J,Halbach M,Schöler HR,Zenke M,Neuss S

Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Immunofluorescence

Wed May 01 00:00:00 EDT 2013

Characterization of pluripotent stem cells.

Nature protocols

Martí M,Mulero L,Pardo C,Morera C,Carrió M,Laricchia-Robbio L,Esteban CR,Izpisua Belmonte JC

PA1-16968 was used in immunocytochemistry to describe all the immunodetection procedures needed to characterize mouse and human stem cell lines in different circumstances.

Fri Feb 01 00:00:00 EST 2013

Human stem/progenitor cells from bone marrow enhance glial differentiation of rat neural stem cells: a role for transforming growth factor β and Notch signaling.

Stem cells and development

Robinson AP,Foraker JE,Ylostalo J,Prockop DJ

PA1-16968 was used in immunocytochemistry to investigate the interaction between human stem/progenitor cells and rat neural stem cells in coculture

Tue Feb 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); sex determining region Y-box 2; SOX 2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY related HMG box 2; SRY-box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2

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Gene Aliases: ANOP3; lcc; MCOPS3; RGD1565646; Sox-2; SOX2; ysb

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UniProt ID: (Human) P48431, (Sheep) P54231, (Mouse) P48432

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Entrez Gene ID: (Human) 6657, (Dog) 488092, (Sheep) 101110563, (Rat) 499593, (Mouse) 20674

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Function(s)

transcription regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding miRNA binding sequence-specific DNA binding transcription regulatory region DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding molecular_function chromatin binding transcription factor binding protein heterodimerization activity chromatin DNA binding HMG box transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation eye development endodermal cell fate specification chromatin organization regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell cycle arrest response to wounding regulation of gene expression glial cell fate commitment pituitary gland development adenohypophysis development positive regulation of cell-cell adhesion forebrain development somatic stem cell population maintenance tissue regeneration regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade positive regulation of cell differentiation negative regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter inner ear development negative regulation of epithelial cell proliferation response to growth factor negative regulation of canonical Wnt signaling pathway neuronal stem cell population maintenance cell fate specification positive regulation of neuroblast proliferation regulation of transcription from RNA polymerase II promoter sensory perception of sound response to organic substance stem cell population maintenance forebrain neuron differentiation cerebral cortex development negative regulation of Wnt signaling pathway male genitalia development positive regulation of epithelial cell differentiation olfactory placode formation response to retinoic acid inner ear morphogenesis tongue development cell fate commitment negative regulation of cell differentiation positive regulation of neuron differentiation negative regulation of osteoblast differentiation positive regulation of Notch signaling pathway pigment biosynthetic process lung alveolus development embryonic organ development anatomical structure formation involved in morphogenesis neuron fate commitment diencephalon morphogenesis stem cell differentiation regulation of neurogenesis detection of mechanical stimulus involved in sensory perception of sound detection of mechanical stimulus involved in equilibrioception retina morphogenesis in camera-type eye lens induction in camera-type eye epithelial tube branching involved in lung morphogenesis transcription, DNA-templated multicellular organism development

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SOX2 Polyclonal Antibody

SOX2 Antibody (PA1-16968) in IHC (P)

Product Details

PA1-16968

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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