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Invitrogen
SOX2 Monoclonal Antibody (20G5), DyLight™ 550
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
SOX2 Antibody (MA1-014-D550) in ICC/IF
Immunofluorescence analysis of SOX2 was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SOX2 Mouse Monoclonal Antibody (Product # MA1-014-D550) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight (Panel a: red). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin ... View More
Product Details
MA1-014-D550
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
20G5
Immunogen
Full-length human recombinant protein expressed in bacteria
Conjugate
Excitation/Emission Max
556/570 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with proprietary stabilizer
Contains
0.02% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536669
Product Specific Information
MA1-014-D550 has been successfully used in ICC/IF applications with human and mouse samples.
Target Information
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); sex determining region Y-box 2; SOX 2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY related HMG box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2
Gene Aliases: ANOP3; lcc; MCOPS3; Sox-2; SOX2; ysb
UniProt ID: (Human) P48431, (Mouse) P48432
Entrez Gene ID: (Human) 6657, (Mouse) 20674
transcription regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
miRNA binding
sequence-specific DNA binding
transcription regulatory region DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
chromatin binding
transcription factor binding
chromatin DNA binding
protein heterodimerization activity
HMG box transcription factor
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
eye development
endodermal cell fate specification
chromatin organization
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cell cycle arrest
response to wounding
regulation of gene expression
glial cell fate commitment
pituitary gland development
adenohypophysis development
positive regulation of cell-cell adhesion
forebrain development
somatic stem cell population maintenance
tissue regeneration
regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of MAPK cascade
positive regulation of cell differentiation
negative regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
inner ear development
negative regulation of epithelial cell proliferation
response to growth factor
negative regulation of canonical Wnt signaling pathway
neuronal stem cell population maintenance
cell fate specification
positive regulation of neuroblast proliferation
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
multicellular organism development
sensory perception of sound
response to organic substance
stem cell population maintenance
forebrain neuron differentiation
cerebral cortex development
negative regulation of Wnt signaling pathway
male genitalia development
positive regulation of epithelial cell differentiation
olfactory placode formation
response to retinoic acid
inner ear morphogenesis
tongue development
cell fate commitment
negative regulation of cell differentiation
positive regulation of neuron differentiation
negative regulation of osteoblast differentiation
positive regulation of Notch signaling pathway
pigment biosynthetic process
lung alveolus development
embryonic organ development
anatomical structure formation involved in morphogenesis
neuron fate commitment
diencephalon morphogenesis
stem cell differentiation
regulation of neurogenesis
detection of mechanical stimulus involved in sensory perception of sound
detection of mechanical stimulus involved in equilibrioception
retina morphogenesis in camera-type eye
lens induction in camera-type eye
epithelial tube branching involved in lung morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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