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SOX2 Antibody (740013TP647) in ICC/IF
Immunofluorescence analysis of SOX2 was performed using 70% confluent NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with SOX2 Recombinant Rat Monoclonal Antibody (Btjce), Alexa Fluor™ Plus 647 (Product # 740013TP647, 1:500) at 4 degree celsius overnight. Panel a) shows representative images of cells that were stained for detection and localization of SOX2. Panel b) shows represen... View More
Product Details
740013TP647
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
Btjce
Conjugate
Excitation/Emission Max
658/675 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein G
Storage buffer
proprietary buffer, pH 6.8
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_3099302
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This Btjce antibody has been tested by immunohistochemistry of mouse brain tissue and immunocytochemistry and flow cytometric analysis of NTERA-2 cells. This may be used for immunocytochemistry at 2 µg/mL and for flow cytometry at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); sex determining region Y-box 2; SOX 2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY related HMG box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2
Gene Aliases: ANOP3; lcc; MCOPS3; Sox-2; SOX2; ysb
UniProt ID: (Human) P48431, (Mouse) P48432
Entrez Gene ID: (Human) 6657, (Mouse) 20674
transcription regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
miRNA binding
sequence-specific DNA binding
transcription regulatory region DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
chromatin binding
transcription factor binding
chromatin DNA binding
protein heterodimerization activity
HMG box transcription factor
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
eye development
endodermal cell fate specification
chromatin organization
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cell cycle arrest
response to wounding
regulation of gene expression
glial cell fate commitment
pituitary gland development
adenohypophysis development
positive regulation of cell-cell adhesion
forebrain development
somatic stem cell population maintenance
tissue regeneration
regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of MAPK cascade
positive regulation of cell differentiation
negative regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
inner ear development
negative regulation of epithelial cell proliferation
response to growth factor
negative regulation of canonical Wnt signaling pathway
neuronal stem cell population maintenance
cell fate specification
positive regulation of neuroblast proliferation
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
multicellular organism development
sensory perception of sound
response to organic substance
stem cell population maintenance
forebrain neuron differentiation
cerebral cortex development
negative regulation of Wnt signaling pathway
male genitalia development
positive regulation of epithelial cell differentiation
olfactory placode formation
response to retinoic acid
inner ear morphogenesis
tongue development
cell fate commitment
negative regulation of cell differentiation
positive regulation of neuron differentiation
negative regulation of osteoblast differentiation
positive regulation of Notch signaling pathway
pigment biosynthetic process
lung alveolus development
embryonic organ development
anatomical structure formation involved in morphogenesis
neuron fate commitment
diencephalon morphogenesis
stem cell differentiation
regulation of neurogenesis
detection of mechanical stimulus involved in sensory perception of sound
detection of mechanical stimulus involved in equilibrioception
retina morphogenesis in camera-type eye
lens induction in camera-type eye
epithelial tube branching involved in lung morphogenesis
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