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SP1 Antibody (MA5-27783) in ICC/IF
SP1 Monoclonal Antibody (GT2574) detects SP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SP1 protein stained by SP1 Monoclonal Antibody (GT2574) (Product # MA5-27783) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Product Details
MA5-27783
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
GT2574
Immunogen
Recombinant protein encompassing a sequence within the center region of human SP1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735369
Product Specific Information
Positive Control: THP-1
Predicted Reactivity: Mouse (96%), Rat (99%), Rhesus Monkey (99%), Bovine (98%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:11205; specificity protein 1; Transcription factor Sp1
Gene Aliases: SP1; TSFP1
UniProt ID: (Human) P08047
Entrez Gene ID: (Human) 6667
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
core promoter sequence-specific DNA binding
RNA polymerase II repressing transcription factor binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein C-terminus binding
transcription factor binding
histone acetyltransferase binding
protein homodimerization activity
histone deacetylase binding
bHLH transcription factor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
metal ion binding
HMG box domain binding
C2H2 zinc finger transcription factor
regulation of transcription, DNA-templated
viral process
snRNA transcription from RNA polymerase II promoter
positive regulation by host of viral transcription
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
regulation of phenotypic switching by transcription from RNA polymerase II promoter
positive regulation of hydrogen sulfide biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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