Proteintech

SPTLC2 Polyclonal Antibody

SPTLC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SPTLC2 Antibody (51012-2-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human colon tissue slide using 51012-2-AP (SPTLC2 antibody) at dilution of 1:200 (under 40x lens).

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Product Details

51012-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:300-1:1,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Target Information

Serine palmitoyltransferase (SPT), which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPT2 is the long chain base subunit 2 of mammalian serine palmitoyltransferase. SPT2 is catalytically active but needs its related protein SPT1 for its stabilization and anchoring the holoenzyme to the cytosolic face of the endoplasmic reticulum. As in the case with SPT1, mutations in the SPT2 gene can cause hereditary sensory and autonomic neuropathy type I (HSAN-I), resulting from a loss of SPT activity as well as the accumulation of the atypical and neurotoxic sphingoid metabolite 1-deoxy-sphinganine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

m⁶A modification-tuned sphingolipid metabolism regulates postnatal liver development in male mice.

Nature metabolism

Wang S,Chen S,Sun J,Han P,Xu B,Li X,Zhong Y,Xu Z,Zhang P,Mi P,Zhang C,Li L,Zhang H,Xia Y,Li S,Heikenwalder M,Yuan D

Mon May 01 00:00:00 EDT 2023

m⁶A modification-tuned sphingolipid metabolism regulates postnatal liver development in male mice.

Nature metabolism

Wang S,Chen S,Sun J,Han P,Xu B,Li X,Zhong Y,Xu Z,Zhang P,Mi P,Zhang C,Li L,Zhang H,Xia Y,Li S,Heikenwalder M,Yuan D

Mon May 01 00:00:00 EDT 2023

SPTSSA variants alter sphingolipid synthesis and cause a complex hereditary spastic paraplegia.

Brain : a journal of neurology

Srivastava S,Shaked HM,Gable K,Gupta SD,Pan X,Somashekarappa N,Han G,Mohassel P,Gotkine M,Doney E,Goldenberg P,Tan QKG,Gong Y,Kleinstiver B,Wishart B,Cope H,Pires CB,Stutzman H,Spillmann RC,Sadjadi R,Elpeleg O,Lee CH,Bellen HJ,Edvardson S,Eichler F,Dunn TM

Wed Apr 19 00:00:00 EDT 2023

Involvement of Huanglian Jiedu Decoction on Microglia with Abnormal Sphingolipid Metabolism in Alzheimer's Disease.

Drug design, development and therapy

Qi YY,Heng X,Yao ZY,Qu SY,Ge PY,Zhao X,Ni SJ,Guo R,Yang NY,Zhang QC,Zhu HX

Mon Apr 11 00:00:00 EDT 2022

Exercise training enhances muscle mitochondrial metabolism in diet-resistant obesity.

EBioMedicine

Pileggi CA,Blondin DP,Hooks BG,Parmar G,Alecu I,Patten DA,Cuillerier A,O'Dwyer C,Thrush AB,Fullerton MD,Bennett SA,Doucet É,Haman F,Cuperlovic-Culf M,McPherson R,Dent RRM,Harper ME

Thu Sep 01 00:00:00 EDT 2022

Peroxisomal L-bifunctional protein (EHHADH) deficiency causes male-specific kidney hypertrophy and proximal tubular injury in mice.

Kidney360

Ranea-Robles P,Portman K,Bender A,Lee K,He JC,Mulholland DJ,Argmann C,Houten SM

Wed Sep 01 00:00:00 EDT 2021

IRE1A Stimulates Hepatocyte-Derived Extracellular Vesicles That Promote Inflammation in Mice With Steatohepatitis.

Gastroenterology

Dasgupta D,Nakao Y,Mauer AS,Thompson JM,Sehrawat TS,Liao CY,Krishnan A,Lucien F,Guo Q,Liu M,Xue F,Fukushima M,Katsumi T,Bansal A,Pandey MK,Maiers JL,DeGrado T,Ibrahim SH,Revzin A,Pavelko KD,Barry MA,Kaufman RJ,Malhi H

Thu Oct 01 00:00:00 EDT 2020

Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function.

Cell death & disease

Li Z,Kabir I,Tietelman G,Huan C,Fan J,Worgall T,Jiang XC

Wed Feb 07 00:00:00 EST 2018

Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function.

Cell death & disease

Li Z,Kabir I,Tietelman G,Huan C,Fan J,Worgall T,Jiang XC

Wed Feb 07 00:00:00 EST 2018

Sphingolipid biosynthesis induces a conformational change in the murine norovirus receptor and facilitates viral infection.

Nature microbiology

Orchard RC,Wilen CB,Virgin HW

Mon Oct 01 00:00:00 EDT 2018

Sphingolipid biosynthesis induces a conformational change in the murine norovirus receptor and facilitates viral infection.

Nature microbiology

Orchard RC,Wilen CB,Virgin HW

Mon Oct 01 00:00:00 EDT 2018

Sphingolipid biosynthesis induces a conformational change in the murine norovirus receptor and facilitates viral infection.

Nature microbiology

Orchard RC,Wilen CB,Virgin HW

Mon Oct 01 00:00:00 EDT 2018

Sphingolipid biosynthesis induces a conformational change in the murine norovirus receptor and facilitates viral infection.

Nature microbiology

Orchard RC,Wilen CB,Virgin HW

Mon Oct 01 00:00:00 EDT 2018

Macrophage-specific de Novo Synthesis of Ceramide Is Dispensable for Inflammasome-driven Inflammation and Insulin Resistance in Obesity.

The Journal of biological chemistry

Camell CD,Nguyen KY,Jurczak MJ,Christian BE,Shulman GI,Shadel GS,Dixit VD

Fri Dec 04 00:00:00 EST 2015

Macrophage-specific de Novo Synthesis of Ceramide Is Dispensable for Inflammasome-driven Inflammation and Insulin Resistance in Obesity.

The Journal of biological chemistry

Camell CD,Nguyen KY,Jurczak MJ,Christian BE,Shulman GI,Shadel GS,Dixit VD

Fri Dec 04 00:00:00 EST 2015

Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome.

Molecular & cellular proteomics : MCP

Campbell-Valois FX,Trost M,Chemali M,Dill BD,Laplante A,Duclos S,Sadeghi S,Rondeau C,Morrow IC,Bell C,Gagnon E,Hatsuzawa K,Thibault P,Desjardins M

Sun Jul 01 00:00:00 EDT 2012

Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome.

Molecular & cellular proteomics : MCP

Campbell-Valois FX,Trost M,Chemali M,Dill BD,Laplante A,Duclos S,Sadeghi S,Rondeau C,Morrow IC,Bell C,Gagnon E,Hatsuzawa K,Thibault P,Desjardins M

Sun Jul 01 00:00:00 EDT 2012

Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome.

Molecular & cellular proteomics : MCP

Campbell-Valois FX,Trost M,Chemali M,Dill BD,Laplante A,Duclos S,Sadeghi S,Rondeau C,Morrow IC,Bell C,Gagnon E,Hatsuzawa K,Thibault P,Desjardins M

Sun Jul 01 00:00:00 EDT 2012

Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome.

Molecular & cellular proteomics : MCP

Campbell-Valois FX,Trost M,Chemali M,Dill BD,Laplante A,Duclos S,Sadeghi S,Rondeau C,Morrow IC,Bell C,Gagnon E,Hatsuzawa K,Thibault P,Desjardins M

Sun Jul 01 00:00:00 EDT 2012

Magnesium deficiency upregulates serine palmitoyl transferase (SPT 1 and SPT 2) in cardiovascular tissues: relationship to serum ionized Mg and cytochrome c.

American journal of physiology. Heart and circulatory physiology

Altura BM,Shah NC,Li Z,Jiang XC,Perez-Albela JL,Altura BT

Wed Sep 01 00:00:00 EDT 2010

Magnesium deficiency upregulates serine palmitoyl transferase (SPT 1 and SPT 2) in cardiovascular tissues: relationship to serum ionized Mg and cytochrome c.

American journal of physiology. Heart and circulatory physiology

Altura BM,Shah NC,Li Z,Jiang XC,Perez-Albela JL,Altura BT

Wed Sep 01 00:00:00 EDT 2010

Liver-specific deficiency of serine palmitoyltransferase subunit 2 decreases plasma sphingomyelin and increases apolipoprotein E levels.

The Journal of biological chemistry

Li Z,Li Y,Chakraborty M,Fan Y,Bui HH,Peake DA,Kuo MS,Xiao X,Cao G,Jiang XC

Fri Sep 25 00:00:00 EDT 2009

Liver-specific deficiency of serine palmitoyltransferase subunit 2 decreases plasma sphingomyelin and increases apolipoprotein E levels.

The Journal of biological chemistry

Li Z,Li Y,Chakraborty M,Fan Y,Bui HH,Peake DA,Kuo MS,Xiao X,Cao G,Jiang XC

Fri Sep 25 00:00:00 EDT 2009

Serine palmitoyl-CoA transferase (SPT) deficiency and sphingolipid levels in mice.

Biochimica et biophysica acta

Hojjati MR,Li Z,Jiang XC

Sat Oct 15 00:00:00 EDT 2005

Serine palmitoyl-CoA transferase (SPT) deficiency and sphingolipid levels in mice.

Biochimica et biophysica acta

Hojjati MR,Li Z,Jiang XC

Sat Oct 15 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: LCB 2; LCB2a; Long chain base biosynthesis protein 2; Long chain base biosynthesis protein 2a; Serine palmitoyltransferase 2; serine palmitoyltransferase, long chain base subunit 2; serine palmitoyltransferase, subunit II; Serine-palmitoyl-CoA transferase 2; SPT 2

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Gene Aliases: AI173915; hLCB2a; HSN1C; KIAA0526; LCB2; LCB2A; mKIAA0526; NSAN1C; SPT2; SPTLC2

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UniProt ID: (Human) O15270, (Mouse) P97363

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Entrez Gene ID: (Human) 9517, (Mouse) 20773

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Function(s)

serine C-palmitoyltransferase activity pyridoxal phosphate binding catalytic activity transferase activity transferase activity, transferring acyl groups transaminase

Process(es)

sphingomyelin biosynthetic process sphingolipid biosynthetic process sphinganine biosynthetic process sphingosine biosynthetic process ceramide biosynthetic process positive regulation of lipophagy lipid metabolic process sphingolipid metabolic process metabolic process biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SPTLC2 Polyclonal Antibody

SPTLC2 Antibody (51012-2-AP) in IHC (P)

Product Details

51012-2-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Target Information

Bioinformatics

Performance Guarantee

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