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SQSTM1 Antibody (MA5-50706) in ICC/IF
Immunocytochemistry analysis of SQSTM1 in A549 cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with SQSTM1 recombinant monoclonal antibody (Product # MA5-50706) with a dilution of 1:50 in 2% negative goat serum overnight at 4 . Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the primary antibody ... View More
Product Details
MA5-50706
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PS00-61
Immunogen
Synthetic peptide within Human SQSTM1/ p62 aa 400 to the C-terminus
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3094157
Product Specific Information
Positive control: PC-12 cell lysate, RAW264.7 cell lysate, PANC-1 cell lysate, mouse liver tissue lysate, rat brain tissue lysate, SH-SY5Y cell lysate, HeLa cell lysate, Hela-si-NT cell lysate, Hela-si-p62#1 cell lysate, Hela-si-p62#2 cell lysate, human colon carcinoma tissue, A549.
Predicted band size: 48 kDa
Subcellular Location: Cytoplasm, Endoplasmic reticulum, Endosome, Lysosome, Nucleus.
Target Information
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; oxidative stress induced; oxidative stress induced like; phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; PKC-zeta-interacting protein; Protein kinase C-zeta-interacting protein; Sequestosome-1; STONE14; Ubiquitin-binding protein p62
Gene Aliases: A170; FTDALS3; ORCA; OSF-6; Osi; OSIL; p60; p62; p62B; PDB3; SQSTM1; STAP; STONE14; ZIP; ZIP3
UniProt ID: (Human) Q13501, (Rat) O08623, (Mouse) Q64337
Entrez Gene ID: (Human) 8878, (Rat) 113894, (Mouse) 18412
protein serine/threonine kinase activity
protein kinase C binding
protein binding
zinc ion binding
protein kinase binding
receptor tyrosine kinase binding
ubiquitin protein ligase binding
SH2 domain binding
identical protein binding
protein homodimerization activity
ubiquitin binding
K63-linked polyubiquitin binding
transcription cofactor activity
metal ion binding
positive regulation of protein phosphorylation
immune system process
ubiquitin-dependent protein catabolic process
autophagy
apoptotic process
macroautophagy
positive regulation of macroautophagy
cell differentiation
regulation of I-kappaB kinase/NF-kappaB signaling
protein heterooligomerization
regulation of mitophagy
mitophagy
protein phosphorylation
response to stress
protein localization
regulation of mitochondrion organization
endosomal transport
intracellular signal transduction
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of growth of symbiont in host
positive regulation of transcription from RNA polymerase II promoter
regulation of Ras protein signal transduction
regulation of protein complex stability
mitophagy in response to mitochondrial depolarization
regulation of nucleic acid-templated transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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