Invitrogen

SREBP1 Monoclonal Antibody (2A4)

6 Published Figures

25 References

View all (22) SREBP1 antibodies

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Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (6)

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SREBP1 Antibody (MA5-11685) in WB

Western blot of SREBP-1 using SREBP-1 Monoclonal Antibody (Product # MA5-11685) on HeLa Cells.

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Product Details

MA5-11685

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 24 publications 24 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Hamster, Human

Published species

Avian, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

2A4

Immunogen

Recombinant protein encoding N-terminal amino acids 301-407 (the bHLH/leucine zipper domain) of human SREBP-1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984077

Product Specific Information

MA5-11685 targets SREBP-1 in WB applications and shows reactivity with Hamster and Human samples.

The MA5-11685 immunogen is recombinant protein encoding N-terminal amino acids 301-407 (the bHLH/leucine zipper domain) of human SREBP-1.

Target Information

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mechanical Study of Jian-Gan-Xiao-Zhi Decoction on Nonalcoholic Fatty Liver Disease Based on Integrated Network Pharmacology and Untargeted Metabolomics.

Evidence-based complementary and alternative medicine : eCAM

Cao YJ,Li HZ,Zhao J,Sun YM,Jin XW,Lv SQ,Luo JY,Fang XX,Wen WB,Liao JB

MA5-11685 was used in Western Blotting to investigate the mechanisms by which Jian-Gan-Xiao-Zhi decoction improve nonalcoholic fatty liver disease.

Tue Jul 19 00:00:00 EDT 2022

A proline metabolism selection system and its application to the engineering of lipid biosynthesis in Chinese hamster ovary cells.

Metabolic engineering communications

Budge JD,Roobol J,Singh G,Mozzanino T,Knight TJ,Povey J,Dean A,Turner SJ,Jaques CM,Young RJ,Racher AJ,Smales CM

Published figure using SREBP1 monoclonal antibody (Product # MA5-11685) in Western Blot

Wed Dec 01 00:00:00 EST 2021

Metabolomic analysis of a selective ABCA1 inducer in obesogenic challenge provides a rationale for therapeutic development.

EBioMedicine

Lewandowski CT,Khan MW,BenAissa M,Dubrovskyi O,Ackerman-Berrier M,LaDu MJ,Layden BT,Thatcher GRJ

MA5-11685 was used in Western Blot to synthesize and characterize a novel small molecule for selective induction of ABCA1 vs.

Thu Apr 01 00:00:00 EDT 2021

Disease-associated mutations in Niemann-Pick type C1 alter ER calcium signaling and neuronal plasticity.

The Journal of cell biology

Tiscione SA,Vivas O,Ginsburg KS,Bers DM,Ory DS,Santana LF,Dixon RE,Dickson EJ

Published figure using SREBP1 monoclonal antibody (Product # MA5-11685) in Western Blot

Mon Dec 02 00:00:00 EST 2019

TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis.

Nature

Hsieh J,Koseki M,Molusky MM,Yakushiji E,Ichi I,Westerterp M,Iqbal J,Chan RB,Abramowicz S,Tascau L,Takiguchi S,Yamashita S,Welch CL,Di Paolo G,Hussain MM,Lefkowitch JH,Rader DJ,Tall AR

MA511685 was used in western blot to describe the ubiquitination and degradation of liver X receptor

Thu Jul 14 00:00:00 EDT 2016

REV-ERBα Activates C/EBP Homologous Protein to Control Small Heterodimer Partner-Mediated Oscillation of Alcoholic Fatty Liver.

The American journal of pathology

Yang Z,Tsuchiya H,Zhang Y,Lee S,Liu C,Huang Y,Vargas GM,Wang L

Published figure using SREBP1 monoclonal antibody (Product # MA5-11685) in Western Blot

Tue Nov 01 00:00:00 EDT 2016

PPARδ activation attenuates hepatic steatosis in Ldlr-/- mice by enhanced fat oxidation, reduced lipogenesis, and improved insulin sensitivity.

Journal of lipid research

Bojic LA,Telford DE,Fullerton MD,Ford RJ,Sutherland BG,Edwards JY,Sawyez CG,Gros R,Kemp BE,Steinberg GR,Huff MW

MA5-11685 was used in western blot to study the roles of decreased lipogenesis, increased beta-oxidation and enhanced insulin-sensitivity in the mechanism by which PPAR-delta protects against hepatic steatosis in LDLR-deficient mice

Tue Jul 01 00:00:00 EDT 2014

The epigenetic drug 5-azacytidine interferes with cholesterol and lipid metabolism.

The Journal of biological chemistry

Poirier S,Samami S,Mamarbachi M,Demers A,Chang TY,Vance DE,Hatch GM,Mayer G

MA5-11685 was used in western blot to study the DNA methylation-independent mechanism by which the epigenetic drug 5-azacytidine inhibits the expression of genes involved in cholesterol and lipid metabolism

Fri Jul 04 00:00:00 EDT 2014

Effect of cholesterol on lipogenesis and VLDL-TG assembly and secretion in goose primary hepatocytes.

Molecular and cellular biochemistry

Han CC,Wang JW,Pan ZX,Tang H,Xiang SX,Wang J,Li L,Xu F,Wei SH

MA5-11685 was used in western blot to study lipogenesis and the assembly and secretion of VLDL-triglyceride in goose primary hepatocytes and the effects of different concentrations of cholesterol

Fri Feb 01 00:00:00 EST 2013

Glucose 6-phosphate, rather than xylulose 5-phosphate, is required for the activation of ChREBP in response to glucose in the liver.

Journal of hepatology

Dentin R,Tomas-Cobos L,Foufelle F,Leopold J,Girard J,Postic C,Ferré P

MA5-11685 was used in western blot to study the role of glucose-6-phosphate as the glucose-signaling metabolite that activates ChREBP in response to hepatic glucose

Sun Jan 01 00:00:00 EST 2012

The lipogenic transcription factor ChREBP dissociates hepatic steatosis from insulin resistance in mice and humans.

The Journal of clinical investigation

Benhamed F,Denechaud PD,Lemoine M,Robichon C,Moldes M,Bertrand-Michel J,Ratziu V,Serfaty L,Housset C,Capeau J,Girard J,Guillou H,Postic C

MA5-11685 was used in western blot to study the role of the lipogenic transcription factor ChREBP in hepatic steatosis and insulin resistance in humans and mice

Fri Jun 01 00:00:00 EDT 2012

Evaluation of Fbxw7 expression and its correlation with expression of SREBP-1 in a mouse model of NAFLD.

Molecular medicine reports

Tu K,Zheng X,Yin G,Zan X,Yao Y,Liu Q

MA5-11685 was used in immunohistochemistry to study the relationship between Fbxw7 and SREBP-1 in a murine model of non-alcoholic fatty liver disease

Sat Sep 01 00:00:00 EDT 2012

Inhibition of intestinal bile acid transporter Slc10a2 improves triglyceride metabolism and normalizes elevated plasma glucose levels in mice.

PloS one

Lundåsen T,Andersson EM,Snaith M,Lindmark H,Lundberg J,Östlund-Lindqvist AM,Angelin B,Rudling M

MA5-11685 was used in western blot to study the beneficial changes in triglyceride metabolism and plasma glucose concentrations of ob/ob obese mice following inhibition of intestinal Slc10a2

Thu Dec 13 00:00:00 EST 2012

The oxysterol 24(s),25-epoxycholesterol attenuates human smooth muscle-derived foam cell formation via reduced low-density lipoprotein uptake and enhanced cholesterol efflux.

Journal of the American Heart Association

Beyea MM,Reaume S,Sawyez CG,Edwards JY,O'Neil C,Hegele RA,Pickering JG,Huff MW

MA5-11685 was used in western blot to study the role of alterations in LDL and cholesterol metabolism in the mechanism by which an oxysterol reduces smooth muscle cell foam cell formation

Fri Jun 01 00:00:00 EDT 2012

Distinct regulation of adiponutrin/PNPLA3 gene expression by the transcription factors ChREBP and SREBP1c in mouse and human hepatocytes.

Journal of hepatology

Dubuquoy C,Robichon C,Lasnier F,Langlois C,Dugail I,Foufelle F,Girard J,Burnol AF,Postic C,Moldes M

MA5-11685 was used in western blot to study the differential roles of ChREBP and SREBP1c in regulating adiponutrin expression in mouse and human hepatocytes

Fri Jul 01 00:00:00 EDT 2011

Species-specific dibutyl phthalate fetal testis endocrine disruption correlates with inhibition of SREBP2-dependent gene expression pathways.

Toxicological sciences : an official journal of the Society of Toxicology

Johnson KJ,McDowell EN,Viereck MP,Xia JQ

MA5-11685 was used in western blot to study the mechanism for the phthalate-induced inhibition of fetal testis steroidogenesis

Fri Apr 01 00:00:00 EDT 2011

The TRC8 ubiquitin ligase is sterol regulated and interacts with lipid and protein biosynthetic pathways.

Molecular cancer research : MCR

Lee JP,Brauweiler A,Rudolph M,Hooper JE,Drabkin HA,Gemmill RM

MA5-11685 was used in western blot to study the role of sterol-regulated TRC8 ubiquitin ligase in regulation of lipid and protein biosynthetic pathways

Fri Jan 01 00:00:00 EST 2010

GRP78 expression inhibits insulin and ER stress-induced SREBP-1c activation and reduces hepatic steatosis in mice.

The Journal of clinical investigation

Kammoun HL,Chabanon H,Hainault I,Luquet S,Magnan C,Koike T,Ferré P,Foufelle F

MA5-11685 was used in western blot to investigate the involvement of endoplasmic reticulum stress in hepatic steatosis and insulin resistance in obese ob/ob mice

Fri May 01 00:00:00 EDT 2009

Hepatitis C virus proteins induce lipogenesis and defective triglyceride secretion in transgenic mice.

The Journal of biological chemistry

Lerat H,Kammoun HL,Hainault I,Mérour E,Higgs MR,Callens C,Lemon SM,Foufelle F,Pawlotsky JM

MA5-11685 was used in western blot to study defects in lipid metabolism in a mouse model expressing human hepatitis C

Fri Nov 27 00:00:00 EST 2009

Regulation of energy substrate utilization and hepatic insulin sensitivity by phosphatidylcholine transfer protein/StarD2.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Scapa EF,Pocai A,Wu MK,Gutierrez-Juarez R,Glenz L,Kanno K,Li H,Biddinger S,Jelicks LA,Rossetti L,Cohen DE

MA5-11685 was used in western blot to examine the role of phosphatidylcholine transfer protein/StarD2 in hepatic insulin sensitivity

Tue Jul 01 00:00:00 EDT 2008

Liver-specific inhibition of ChREBP improves hepatic steatosis and insulin resistance in ob/ob mice.

Diabetes

Dentin R,Benhamed F,Hainault I,Fauveau V,Foufelle F,Dyck JR,Girard J,Postic C

MA5-11685 was used in western blot to study the beneficial effects of shRNA-mediated knockdown of ChREBP on hepatic steatosis and insulin sensitivity in ob/ob leptin-deficient mice

Tue Aug 01 00:00:00 EDT 2006

Insulin regulation of glucokinase gene expression: evidence against a role for sterol regulatory element binding protein 1 in primary hepatocytes.

FEBS letters

Gregori C,Guillet-Deniau I,Girard J,Decaux JF,Pichard AL

MA5-11685 was used in western blot to study the lack of SREBP-1 involvement in the regulation of glucokinase gene expression in primary hepatocytes

Mon Jan 23 00:00:00 EST 2006

Polyunsaturated fatty acids suppress glycolytic and lipogenic genes through the inhibition of ChREBP nuclear protein translocation.

The Journal of clinical investigation

Dentin R,Benhamed F,Pégorier JP,Foufelle F,Viollet B,Vaulont S,Girard J,Postic C

MA5-11685 was used in western blot to study the mechanism by which polyunsaturated fatty acids suppress the expresson of genes involved in glycolysis and lipogenesis

Sat Oct 01 00:00:00 EDT 2005

Hepatic farnesyl diphosphate synthase expression is suppressed by polyunsaturated fatty acids.

The Biochemical journal

Le Jossic-Corcos C,Gonthier C,Zaghini I,Logette E,Shechter I,Bournot P

MA5-11685 was used in western blot to study the mechanism by which polyunsaturated fatty acids suppress liver farnesyl diphosphate synthase expression

Tue Feb 01 00:00:00 EST 2005

Hepatic glucokinase is required for the synergistic action of ChREBP and SREBP-1c on glycolytic and lipogenic gene expression.

The Journal of biological chemistry

Dentin R,Pégorier JP,Benhamed F,Foufelle F,Ferré P,Fauveau V,Magnuson MA,Girard J,Postic C

MA5-11685 was used in western blot to study the role of hepatic glucokinase in the synergistic action of ChREBP and SREBP-1c on glycolytic and lipogenic gene expression

Fri May 07 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: bHLHd1; Class D basic helix-loop-helix protein 1; nt-SREBP1; SREBP-1; sterol regulatory element binding protein-1; Sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1

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Gene Aliases: BHLHD1; I79_010155; SREBF1; SREBP-1; SREBP-1c; SREBP1; SREBP1a

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UniProt ID: (Human) P36956, (Chinese hamster) Q60416

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Entrez Gene ID: (Human) 6720, (Chinese hamster) 100689018

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding protein kinase binding protein complex binding sterol response element binding protein dimerization activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter regulation of heart rate by chemical signal regulation of transcription from RNA polymerase II promoter lipid metabolic process aging circadian rhythm cholesterol metabolic process insulin receptor signaling pathway lipid biosynthetic process cellular response to starvation response to glucose positive regulation of triglyceride biosynthetic process regulation of fatty acid metabolic process lung development positive regulation of histone deacetylation regulation of protein stability response to food response to retinoic acid response to progesterone response to glucagon response to drug mRNA transcription from RNA polymerase II promoter fat cell differentiation positive regulation of cholesterol biosynthetic process positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion response to cAMP cellular response to fatty acid regulation of mitophagy regulation of protein targeting to mitochondrion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SREBP1 Monoclonal Antibody (2A4)

SREBP1 Antibody (MA5-11685) in WB

Product Details

MA5-11685

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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