Invitrogen

SREBP1 Monoclonal Antibody (2A4)

2 Published Figures

23 References

View all (22) SREBP1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (2)

FIGURE: 1 / 3

SREBP1 Antibody (MA5-16124) in WB

Western blot analysis of SREBP1 using a monoclonal antibody (Product # MA5-16124).

Product Image Gallery

Product Details

MA5-16124

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 21 publications 21 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Chicken, Hamster, Human, Mouse, Rat

Published species

Avian, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

2A4

Immunogen

6 His-tag fusion protein of SREBP1 corresponding to amino acids 301-407

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152118

Product Specific Information

In Western blot, a band can be seen at 125 kDa (precursor) and additional bands may be seen at 60-70 kDa (cleaved).

Suggested positive control: IMR 5 and HeLa cells..

Target Information

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Isthmin-1 is an adipokine that promotes glucose uptake and improves glucose tolerance and hepatic steatosis.

Cell metabolism

Jiang Z,Zhao M,Voilquin L,Jung Y,Aikio MA,Sahai T,Dou FY,Roche AM,Carcamo-Orive I,Knowles JW,Wabitsch M,Appel EA,Maikawa CL,Camporez JP,Shulman GI,Tsai L,Rosen ED,Gardner CD,Spiegelman BM,Svensson KJ

MA5-16124 was used in Western Blotting to identify isthmin-1 (Ism1) as an adipokine and one that has a dual role in increasing adipose glucose uptake while suppressing hepatic lipid synthesis.

Tue Sep 07 00:00:00 EDT 2021

Isthmin-1 is an adipokine that promotes glucose uptake and improves glucose tolerance and hepatic steatosis.

Cell metabolism

Jiang Z,Zhao M,Voilquin L,Jung Y,Aikio MA,Sahai T,Dou FY,Roche AM,Carcamo-Orive I,Knowles JW,Wabitsch M,Appel EA,Maikawa CL,Camporez JP,Shulman GI,Tsai L,Rosen ED,Gardner CD,Spiegelman BM,Svensson KJ

MA5-16124 was used in Western Blotting to identify isthmin-1 (Ism1) as an adipokine and one that has a dual role in increasing adipose glucose uptake while suppressing hepatic lipid synthesis.

Tue Sep 07 00:00:00 EDT 2021

Protein Arginine Methyltransferase PRMT5 Regulates Fatty Acid Metabolism and Lipid Droplet Biogenesis in White Adipose Tissues.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Jia Z,Yue F,Chen X,Narayanan N,Qiu J,Syed SA,Imbalzano AN,Deng M,Yu P,Hu C,Kuang S

MA5-16124 was used in Western Blotting to conclude that PRMT5 plays a critical role in regulating lipid metabolism and lipid droplet biogenesis in adipocytes.

Tue Dec 01 00:00:00 EST 2020

Protein Arginine Methyltransferase PRMT5 Regulates Fatty Acid Metabolism and Lipid Droplet Biogenesis in White Adipose Tissues.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Jia Z,Yue F,Chen X,Narayanan N,Qiu J,Syed SA,Imbalzano AN,Deng M,Yu P,Hu C,Kuang S

MA5-16124 was used in Western Blotting to conclude that PRMT5 plays a critical role in regulating lipid metabolism and lipid droplet biogenesis in adipocytes.

Tue Dec 01 00:00:00 EST 2020

TNF-Receptor-1 inhibition reduces liver steatosis, hepatocellular injury and fibrosis in NAFLD mice.

Cell death & disease

Wandrer F,Liebig S,Marhenke S,Vogel A,John K,Manns MP,Teufel A,Itzel T,Longerich T,Maier O,Fischer R,Kontermann RE,Pfizenmaier K,Schulze-Osthoff K,Bantel H

Published figure using SREBP1 monoclonal antibody (Product # MA5-16124) in Western Blot

Tue Mar 31 00:00:00 EDT 2020

TNF-Receptor-1 inhibition reduces liver steatosis, hepatocellular injury and fibrosis in NAFLD mice.

Cell death & disease

Wandrer F,Liebig S,Marhenke S,Vogel A,John K,Manns MP,Teufel A,Itzel T,Longerich T,Maier O,Fischer R,Kontermann RE,Pfizenmaier K,Schulze-Osthoff K,Bantel H

Published figure using SREBP1 monoclonal antibody (Product # MA5-16124) in Western Blot

Tue Mar 31 00:00:00 EDT 2020

Treg Cells Promote the SREBP1-Dependent Metabolic Fitness of Tumor-Promoting Macrophages via Repression of CD8⁺ T Cell-Derived Interferon-γ.

Immunity

Liu C,Chikina M,Deshpande R,Menk AV,Wang T,Tabib T,Brunazzi EA,Vignali KM,Sun M,Stolz DB,Lafyatis RA,Chen W,Delgoffe GM,Workman CJ,Wendell SG,Vignali DAA

MA5-16124 was used in Flow cytometry/Cell sorting to show that Treg cells shape the transcriptional landscape across multiple tumor-infiltrating immune cell types.

Tue Aug 20 00:00:00 EDT 2019

TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis.

Nature

Hsieh J,Koseki M,Molusky MM,Yakushiji E,Ichi I,Westerterp M,Iqbal J,Chan RB,Abramowicz S,Tascau L,Takiguchi S,Yamashita S,Welch CL,Di Paolo G,Hussain MM,Lefkowitch JH,Rader DJ,Tall AR

MA516124 was used in western blot to describe the ubiquitination and degradation of liver X receptor

Thu Jul 14 00:00:00 EDT 2016

Reprogramming towards anabolism impedes degeneration in a preclinical model of retinitis pigmentosa.

Human molecular genetics

Zhang L,Justus S,Xu Y,Pluchenik T,Hsu CW,Yang J,Duong JK,Lin CS,Jia Y,Bassuk AG,Mahajan VB,Tsang SH

MA516124 was used in western blot to assess a non-gene-specific strategy to reprogram photoreceptors towards anabolism by upregulating the mechanistic target of rapamycin pathway

Sat Oct 01 00:00:00 EDT 2016

PPARδ activation attenuates hepatic steatosis in Ldlr-/- mice by enhanced fat oxidation, reduced lipogenesis, and improved insulin sensitivity.

Journal of lipid research

Bojic LA,Telford DE,Fullerton MD,Ford RJ,Sutherland BG,Edwards JY,Sawyez CG,Gros R,Kemp BE,Steinberg GR,Huff MW

MA5-16124 was used in western blot to study the roles of decreased lipogenesis, increased beta-oxidation and enhanced insulin-sensitivity in the mechanism by which PPAR-delta protects against hepatic steatosis in LDLR-deficient mice

Tue Jul 01 00:00:00 EDT 2014

The epigenetic drug 5-azacytidine interferes with cholesterol and lipid metabolism.

The Journal of biological chemistry

Poirier S,Samami S,Mamarbachi M,Demers A,Chang TY,Vance DE,Hatch GM,Mayer G

MA5-16124 was used in western blot to study the DNA methylation-independent mechanism by which the epigenetic drug 5-azacytidine inhibits the expression of genes involved in cholesterol and lipid metabolism

Fri Jul 04 00:00:00 EDT 2014

Effect of cholesterol on lipogenesis and VLDL-TG assembly and secretion in goose primary hepatocytes.

Molecular and cellular biochemistry

Han CC,Wang JW,Pan ZX,Tang H,Xiang SX,Wang J,Li L,Xu F,Wei SH

MA5-16124 was used in western blot to study lipogenesis and the assembly and secretion of VLDL-triglyceride in goose primary hepatocytes and the effects of different concentrations of cholesterol

Fri Feb 01 00:00:00 EST 2013

Lack of liver X receptors leads to cell proliferation in a model of mouse dorsal prostate epithelial cell.

PloS one

Dufour J,Pommier A,Alves G,De Boussac H,Lours-Calet C,Volle DH,Lobaccaro JM,Baron S

MA5-16124 was used in western blot to study altered signaling pathways in prostate cells isolated from Lxralphabeta knockout mice

Thu Oct 17 00:00:00 EDT 2013

Glucose 6-phosphate, rather than xylulose 5-phosphate, is required for the activation of ChREBP in response to glucose in the liver.

Journal of hepatology

Dentin R,Tomas-Cobos L,Foufelle F,Leopold J,Girard J,Postic C,Ferré P

MA5-16124 was used in western blot to study the role of glucose-6-phosphate as the glucose-signaling metabolite that activates ChREBP in response to hepatic glucose

Sun Jan 01 00:00:00 EST 2012

The lipogenic transcription factor ChREBP dissociates hepatic steatosis from insulin resistance in mice and humans.

The Journal of clinical investigation

Benhamed F,Denechaud PD,Lemoine M,Robichon C,Moldes M,Bertrand-Michel J,Ratziu V,Serfaty L,Housset C,Capeau J,Girard J,Guillou H,Postic C

MA5-16124 was used in western blot to study the role of the lipogenic transcription factor ChREBP in hepatic steatosis and insulin resistance in humans and mice

Fri Jun 01 00:00:00 EDT 2012

Evaluation of Fbxw7 expression and its correlation with expression of SREBP-1 in a mouse model of NAFLD.

Molecular medicine reports

Tu K,Zheng X,Yin G,Zan X,Yao Y,Liu Q

MA5-16124 was used in immunohistochemistry to study the relationship between Fbxw7 and SREBP-1 in a murine model of non-alcoholic fatty liver disease

Sat Sep 01 00:00:00 EDT 2012

Inhibition of intestinal bile acid transporter Slc10a2 improves triglyceride metabolism and normalizes elevated plasma glucose levels in mice.

PloS one

Lundåsen T,Andersson EM,Snaith M,Lindmark H,Lundberg J,Östlund-Lindqvist AM,Angelin B,Rudling M

MA5-16124 was used in western blot to study the beneficial changes in triglyceride metabolism and plasma glucose concentrations of ob/ob obese mice following inhibition of intestinal Slc10a2

Thu Dec 13 00:00:00 EST 2012

The oxysterol 24(s),25-epoxycholesterol attenuates human smooth muscle-derived foam cell formation via reduced low-density lipoprotein uptake and enhanced cholesterol efflux.

Journal of the American Heart Association

Beyea MM,Reaume S,Sawyez CG,Edwards JY,O'Neil C,Hegele RA,Pickering JG,Huff MW

MA5-16124 was used in western blot to study the role of alterations in LDL and cholesterol metabolism in the mechanism by which an oxysterol reduces smooth muscle cell foam cell formation

Fri Jun 01 00:00:00 EDT 2012

Distinct regulation of adiponutrin/PNPLA3 gene expression by the transcription factors ChREBP and SREBP1c in mouse and human hepatocytes.

Journal of hepatology

Dubuquoy C,Robichon C,Lasnier F,Langlois C,Dugail I,Foufelle F,Girard J,Burnol AF,Postic C,Moldes M

MA5-16124 was used in western blot to study the differential roles of ChREBP and SREBP1c in regulating adiponutrin expression in mouse and human hepatocytes

Fri Jul 01 00:00:00 EDT 2011

The TRC8 ubiquitin ligase is sterol regulated and interacts with lipid and protein biosynthetic pathways.

Molecular cancer research : MCR

Lee JP,Brauweiler A,Rudolph M,Hooper JE,Drabkin HA,Gemmill RM

MA5-16124 was used in western blot to study the role of sterol-regulated TRC8 ubiquitin ligase in regulation of lipid and protein biosynthetic pathways

Fri Jan 01 00:00:00 EST 2010

Regulation of energy substrate utilization and hepatic insulin sensitivity by phosphatidylcholine transfer protein/StarD2.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Scapa EF,Pocai A,Wu MK,Gutierrez-Juarez R,Glenz L,Kanno K,Li H,Biddinger S,Jelicks LA,Rossetti L,Cohen DE

MA5-16124 was used in western blot to examine the role of phosphatidylcholine transfer protein/StarD2 in hepatic insulin sensitivity

Tue Jul 01 00:00:00 EDT 2008

Liver-specific inhibition of ChREBP improves hepatic steatosis and insulin resistance in ob/ob mice.

Diabetes

Dentin R,Benhamed F,Hainault I,Fauveau V,Foufelle F,Dyck JR,Girard J,Postic C

MA5-16124 was used in western blot to study the beneficial effects of shRNA-mediated knockdown of ChREBP on hepatic steatosis and insulin sensitivity in ob/ob leptin-deficient mice

Tue Aug 01 00:00:00 EDT 2006

Insulin regulation of glucokinase gene expression: evidence against a role for sterol regulatory element binding protein 1 in primary hepatocytes.

FEBS letters

Gregori C,Guillet-Deniau I,Girard J,Decaux JF,Pichard AL

MA5-16124 was used in western blot to study the lack of SREBP-1 involvement in the regulation of glucokinase gene expression in primary hepatocytes

Mon Jan 23 00:00:00 EST 2006

Polyunsaturated fatty acids suppress glycolytic and lipogenic genes through the inhibition of ChREBP nuclear protein translocation.

The Journal of clinical investigation

Dentin R,Benhamed F,Pégorier JP,Foufelle F,Viollet B,Vaulont S,Girard J,Postic C

MA5-16124 was used in western blot to study the mechanism by which polyunsaturated fatty acids suppress the expresson of genes involved in glycolysis and lipogenesis

Sat Oct 01 00:00:00 EDT 2005

Hepatic farnesyl diphosphate synthase expression is suppressed by polyunsaturated fatty acids.

The Biochemical journal

Le Jossic-Corcos C,Gonthier C,Zaghini I,Logette E,Shechter I,Bournot P

MA5-16124 was used in western blot to study the mechanism by which polyunsaturated fatty acids suppress liver farnesyl diphosphate synthase expression

Tue Feb 01 00:00:00 EST 2005

Hepatic glucokinase is required for the synergistic action of ChREBP and SREBP-1c on glycolytic and lipogenic gene expression.

The Journal of biological chemistry

Dentin R,Pégorier JP,Benhamed F,Foufelle F,Ferré P,Fauveau V,Magnuson MA,Girard J,Postic C

MA5-16124 was used in western blot to study the role of hepatic glucokinase in the synergistic action of ChREBP and SREBP-1c on glycolytic and lipogenic gene expression

Fri May 07 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: ADD1; Adipocyte determination- and differentiation-dependent factor 1; bHLHd1; Class D basic helix-loop-helix protein 1; nt-SREBP1; SREBP-1; SREBP1; sterol regulatory element binding protein 1; sterol regulatory element binding protein-1; sterol regulatory element binding-protein 1; Sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1

View more View less

Gene Aliases: ADD-1; ADD1; BHLHD1; D630008H06; I79_010155; SREBF1; SREBP-1; SREBP-1a; SREBP-1c; SREBP1; SREBP1a; SREBP1c

View more View less

UniProt ID: (Human) P36956, (Rat) P56720, (Chinese hamster) Q60416, (Mouse) Q9WTN3

View more View less

Entrez Gene ID: (Human) 6720, (Chicken) 373915, (Dog) 403475, (Rat) 78968, (Chinese hamster) 100689018, (Mouse) 20787

View more View less

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding protein kinase binding protein complex binding sterol response element binding protein dimerization activity sequence-specific DNA binding transcription regulatory region DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter regulation of heart rate by chemical signal regulation of transcription from RNA polymerase II promoter lipid metabolic process aging circadian rhythm cholesterol metabolic process insulin receptor signaling pathway lipid biosynthetic process cellular response to starvation response to glucose positive regulation of triglyceride biosynthetic process regulation of fatty acid metabolic process lung development positive regulation of histone deacetylation regulation of protein stability response to food response to retinoic acid response to progesterone response to glucagon response to drug mRNA transcription from RNA polymerase II promoter fat cell differentiation positive regulation of cholesterol biosynthetic process positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion response to cAMP cellular response to fatty acid regulation of mitophagy regulation of protein targeting to mitochondrion transcription, DNA-templated regulation of transcription, DNA-templated response to organic cyclic compound response to insulin cellular response to insulin stimulus response to lipid response to peptide hormone positive regulation of transcription, DNA-templated regulation of insulin secretion response to fatty acid transcription from RNA polymerase II promoter steroid metabolic process positive regulation of fatty acid biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

SREBP1 Monoclonal Antibody (2A4)

SREBP1 Antibody (MA5-16124) in WB

Product Details

MA5-16124

Western Blot (WB)

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!