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SRSF1 Antibody (PA5-102454) in ICC/IF
Immunocytochemistry analysis of SRSF1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SRSF1 polyclonal antibody (Product # PA5-102454) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-102454
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SRSF1(Accession Q07955), corresponding to amino acid residues P54-G104.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2851859
Product Specific Information
Antibody detects endogenous levels of total SFRS1.
Target Information
UBE2E2 catalyzes the covalent attachment of ubiquitin to other proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alternative-splicing factor 1; ASF-1; ASF/SF2; pre-mRNA-splicing factor SF2, P33 subunit; Pre-mRNA-splicing factor SRp30a; Serine/arginine-rich splicing factor 1; splicing factor 2; Splicing factor, arginine/serine-rich 1; splicing factor, arginine/serine-rich 1 (ASF/SF2); splicing factor, arginine/serine-rich 1B; splicing factor, arginine/serine-rich, 30-KD, A; SR splicing factor 1
Gene Aliases: 1110054N12Rik; 5730507C05Rik; 6330415C05Rik; AI482334; ASF; AW491331; OK/SW-cl.3; SF2; SF2P33; SFRS1; SRp30a; SRSF1
UniProt ID: (Human) Q07955, (Mouse) Q5SXC5
Entrez Gene ID: (Human) 6426, (Rat) 689890, (Mouse) 110809
alternative mRNA splicing, via spliceosome
mRNA 5'-splice site recognition
mRNA splicing, via spliceosome
in utero embryonic development
termination of RNA polymerase II transcription
mRNA splice site selection
mRNA processing
RNA export from nucleus
mRNA export from nucleus
mRNA 3'-end processing
cardiac muscle contraction
regulation of transcription, DNA-templated
regulation of translation
RNA splicing
regulation of mRNA stability
regulation of mRNA splicing, via spliceosome
transport
mRNA transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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