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Invitrogen
SSEA1 Monoclonal Antibody (4E10)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
SSEA1 Antibody (MA5-17042) in Flow
THP-1 and Ramos cells were fixed and permeabilized using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-000) and then stained with SSEA1 Monoclonal Antibody (4E10) (Product # MA5-17042) (red histogram) at 1:100 dilution and CD19 Monoclonal Antibody (HIB19), FITC, eBioscience™ (Product # 11-0199-42) (green histogram) at a concentartion of 1 µg or left unstained (black histogram), followed by Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ Plus 647 (Product # A55060, 1 µg/mL) ... View More
Product Details
MA5-17042
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4E10
Immunogen
Synthesized peptide of human CD15.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2538514
Product Specific Information
MA5-17042 targets CD15 in indirect ELISA, ICC, IHC, and IF applications and shows reactivity with Human samples.
The MA5-17042 immunogen is synthesized peptide of human CD15.
MA5-17042 detects CD15 which has a predicted molecular weight of approximately 59kDa.
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid-specific; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; SSEA-1; stage-specific embryonic antigen 1
Gene Aliases: CD15; ELFT; FCT3A; FUC-TIV; FUT4; FUTIV; LeX; SSEA-1
UniProt ID: (Human) P22083
Entrez Gene ID: (Human) 2526
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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