Invitrogen

SSEA3 Monoclonal Antibody (MC-631)

SSEA3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

SSEA3 Antibody (MA1-020) in ICC/IF

Immunofluorescent analysis of SSEA-3 using anti-SSEA-3 monoclonal antibody (Product # MA1-020) shows expression in human teratocarcinoma NCCIT cells (shown in green) but not in negative control HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a rat monoclonal antibody recognizing SSEA-3 (Product # MA1-020), at a dilution of 1:50 for at least 1 hour at room t... View More

Product Image Gallery

Product Details

MA1-020

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:100-1:500

View 3 publications 3 publications

Flow Cytometry (Flow)

1:100

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Deer, Human, Mammal

Host/Isotype

Rat / IgM

Class

Monoclonal

Type

Antibody

Clone

MC-631

Immunogen

Fischer rat immunized with 4-8 cell stage mouse embryos

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography - MBP

Storage buffer

potassium phosphate with 0.15M NaCl, 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2536682

Target Information

SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Generation of a human control iPS cell line (ESi080-A) from a donor with no rheumatic diseases.

Stem cell research

Castro-Viñuelas R,Sanjurjo-Rodríguez C,Piñeiro-Ramil M,Rodríguez-Fernández S,Fuentes-Boquete IM,Blanco FJ,Díaz-Prado SM

MA1-020 was used in Immunocytochemistry-immunoflourescence to report the establishment of the human iPS cell line N1-FiPS4F#7 generated from skin cells of a patient with no rheumatic diseases, thus obtaining an appropriate control iPS cell line for researchers working in the field of rheumatic diseases.

Sun Mar 01 00:00:00 EST 2020

Quantitative Analysis of SSEA3+ Cells from Human Umbilical Cord after Magnetic Sorting.

Cell transplantation

Leng Z,Sun D,Huang Z,Tadmori I,Chiang N,Kethidi N,Sabra A,Kushida Y,Fu YS,Dezawa M,He X,Young W

MA1-020 was used in Flow cytometry/Cell sorting to allow the practical and efficient isolation and expansion of relatively pure populations of SSEA3+ Muse cells that can be matched by human leukocyte antigen for transplantation in human trials.

Mon Jul 01 00:00:00 EDT 2019

Quantitative Analysis of SSEA3+ Cells from Human Umbilical Cord after Magnetic Sorting.

Cell transplantation

Leng Z,Sun D,Huang Z,Tadmori I,Chiang N,Kethidi N,Sabra A,Kushida Y,Fu YS,Dezawa M,He X,Young W

Mon Jul 01 00:00:00 EDT 2019

Embryos and embryonic stem cells from the white rhinoceros.

Nature communications

Hildebrandt TB,Hermes R,Colleoni S,Diecke S,Holtze S,Renfree MB,Stejskal J,Hayashi K,Drukker M,Loi P,Göritz F,Lazzari G,Galli C

MA1-020X was used in Immunocytochemistry-immunoflourescence to adapt existing assisted reproduction techniques (ART) to fertilize Southern White Rhinoceros (SWR) oocytes with NWR spermatozoa.

Wed Jul 04 00:00:00 EDT 2018

Stage-specific embryonic antigen-3 (SSEA-3) and β3GalT5 are cancer specific and significant markers for breast cancer stem cells.

Proceedings of the National Academy of Sciences of the United States of America

Cheung SK,Chuang PK,Huang HW,Hwang-Verslues WW,Cho CH,Yang WB,Shen CN,Hsiao M,Hsu TL,Chang CF,Wong CH

MA1020 was used in flow cytometry to find that breast cancer stem cells isolated with CD44(+)CD24(-/lo)SSEA-3(+) or ESA(hi)PROCR(hi)SSEA-3(+) markers have higher tumorigenicity than those with conventional markers

Tue Jan 26 00:00:00 EST 2016

Characterization of moose intestinal glycosphingolipids.

Glycoconjugate journal

Johansson MM,Dedic B,Lundholm K,Branzell FB,Barone A,Benktander J,Teneberg S

MA1-020 was used in Chromatogram binding assays to isolate and identify glycolipids from three small intestines and one large intestine of the moose.

Sat Aug 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: SSEA-3; stage-specific embryonic antigen 3

View more View less

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more