Invitrogen

SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), PE, eBioscience™

FIGURE: 1 / 5

SSEA4 Antibody (12-8843-42) in Flow

Staining of the 2102Ep cell line with Mouse IgG3 Isotype Control PE (Product # 12-4742-42) (open histogram) or Anti-Human SSEA-4 PE (filled histogram). Total viable cells were used for analysis.

Product Image Gallery

Product Details

12-8843-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 14 publications 14 publications

Product Specifications

Species Reactivity

Dog, Chicken, Human, Mouse, Non-human primate, Rabbit

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG3

Recommended Isotype Control

Mouse IgG3 Isotype Control (B10), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioMC-813-70 (MC-813-70)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11151520

Product Specific Information

Description: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells.

The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys.

Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Neighbor-specific gene expression revealed from physically interacting cells during mouse embryonic development.

Proceedings of the National Academy of Sciences of the United States of America

Kim J,Rothová MM,Madan E,Rhee S,Weng G,Palma AM,Liao L,David E,Amit I,Hajkarim MC,Vudatha V,Gutiérrez-García A,Moreno E,Winn R,Trevino J,Fisher PB,Brickman JM,Gogna R,Won KJ

12-8843-42 was used in Flow cytometry/Cell sorting to describe an innovative approach to study contact-specific gene regulation during embryogenesis.

Tue Jan 10 00:00:00 EST 2023

Human induced pluripotent stem cells ameliorate hyperoxia-induced lung injury in a mouse model.

American journal of translational research

Mitchell A,Wanczyk H,Jensen T,Finck C

12-8843 was used in Flow cytometry/Cell sorting to determine if iPSCs and diPSCs could repair hyperoxia-induced lung damage in a mouse model.

Mon Sep 28 00:00:00 EDT 2020

Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals.

Stem cell research

Xu X,Pradhan M,Xu M,Cheng YS,Beers J,Linask KL,Lin Y,Zheng W,Zou J

Published figure using SSEA4 monoclonal antibody (Product # 12-8843-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates.

Cell reports

Patananan AN,Sercel AJ,Wu TH,Ahsan FM,Torres A,Kennedy SAL,Vandiver A,Collier AJ,Mehrabi A,Van Lew J,Zakin L,Rodriguez N,Sixto M,Tadros W,Lazar A,Sieling PA,Nguyen TL,Dawson ER,Braas D,Golovato J,Cisneros L,Vaske C,Plath K,Rabizadeh S,Niazi KR,Chiou PY,Teitell MA

12-8843-42 was used in flow cytometry to provide a proof-of-principle mitochondrial transfer pipeline to generate cells of different fates with specific mtDNA-nDNA combinations, including clonal lines with genome pairings not found in nature.

Tue Dec 29 00:00:00 EST 2020

Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells.

Stem cells and development

Fujii S,Yoshida S,Inagaki E,Hatou S,Tsubota K,Takahashi M,Shimmura S,Sugita S

Published figure using SSEA4 monoclonal antibody (Product # 12-8843-42) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

An induced pluripotent stem cell line (TRNDi006-A) from a MPS IIIB patient carrying homozygous mutation of p.Glu153Lys in the NAGLU gene.

Stem cell research

Huang W,Xu M,Li R,Baskfield A,Kouznetsova J,Beers J,Zou J,Liu C,Zheng W

Published figure using SSEA4 monoclonal antibody (Product # 12-8843-42) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

Generation of an induced pluripotent stem cell line (TRNDi008-A) from a Hunter syndrome patient carrying a hemizygous 208insC mutation in the IDS gene.

Stem cell research

Hong J,Xu M,Li R,Cheng YS,Kouznetsova J,Beers J,Liu C,Zou J,Zheng W

Published figure using SSEA4 monoclonal antibody (Product # 12-8843-42) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

Low-density lipoprotein receptor-deficient hepatocytes differentiated from induced pluripotent stem cells allow familial hypercholesterolemia modeling, CRISPR/Cas-mediated genetic correction, and productive hepatitis C virus infection.

Stem cell research & therapy

Caron J,Pène V,Tolosa L,Villaret M,Luce E,Fourrier A,Heslan JM,Saheb S,Bruckert E,Gómez-Lechón MJ,Nguyen TH,Rosenberg AR,Weber A,Dubart-Kupperschmitt A

12-8843-42 was used in Flow Cytometry to provides the first LDLR-null FH cell model and its corrected counterpart to study the regulation of cholesterol metabolism and host determinants of HCV life cycle, and a platform to screen drugs for treating dyslipidemia and HCV infection.

Mon Jul 29 00:00:00 EDT 2019

Multiomics Analyses of HNF4α Protein Domain Function during Human Pluripotent Stem Cell Differentiation.

iScience

Wang Y,Tatham MH,Schmidt-Heck W,Swann C,Singh-Dolt K,Meseguer-Ripolles J,Lucendo-Villarin B,Kunath T,Rudd TR,Smith AJH,Hengstler JG,Godoy P,Hay RT,Hay DC

12-8843-42 was used in Flow Cytometry to demonstrate that nuclear HNF4α is essential for hepatic progenitor specification, and the introduction of point mutations in HNF4α's Small Ubiquitin-like Modifier consensus motif leads to disrupted hepatocyte differentiation.

Fri Jun 28 00:00:00 EDT 2019

Simplified Footprint-Free Cas9/CRISPR Editing of Cardiac-Associated Genes in Human Pluripotent Stem Cells.

Stem cells and development

Kondrashov A,Duc Hoang M,Smith JGW,Bhagwan JR,Duncan G,Mosqueira D,Munoz MB,Vo NTN,Denning C

Published figure using SSEA4 monoclonal antibody (Product # 12-8843-42) in Flow Cytometry

Thu Mar 15 00:00:00 EDT 2018

Drug-Mediated Shortening of Action Potentials in LQTS2 Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Stem cells and development

Duncan G,Firth K,George V,Hoang MD,Staniforth A,Smith G,Denning C

12-8843 was used in Flow cytometry/Cell sorting to investigate the importance of drug-mediated shortening of action potentials in cardiomyocytes from patients with LQTS2.

Fri Dec 01 00:00:00 EST 2017

Generation of iPS Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors.

Methods in molecular biology (Clifton, N.J.)

Su RJ,Neises A,Zhang XB

12-8843-42 was used in Flow cytometry/Cell sorting to describe the detailed protocol for the efficient generation of integration-free iPS cells from peripheral blood mononuclear cells.

Fri Oct 14 00:00:00 EDT 2016

Generation of iPS Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors.

Methods in molecular biology (Clifton, N.J.)

Su RJ,Neises A,Zhang XB

12-8843-42 was used in Flow cytometry/Cell sorting to describe the detailed protocol for the efficient generation of integration-free iPS cells from peripheral blood mononuclear cells.

Fri Oct 14 00:00:00 EDT 2016

Toward Personalized Medicine: Using Cardiomyocytes Differentiated From Urine-Derived Pluripotent Stem Cells to Recapitulate Electrophysiological Characteristics of Type 2 Long QT Syndrome.

Journal of the American Heart Association

Jouni M,Si-Tayeb K,Es-Salah-Lamoureux Z,Latypova X,Champon B,Caillaud A,Rungoat A,Charpentier F,Loussouarn G,Baró I,Zibara K,Lemarchand P,Gaborit N

12-8843 was used in Flow cytometry/Cell sorting to evaluate the use of cardiomyocytes differentiated from urine-derived stem cells as a new cellular model for studying patients' specific arrhythmia mechanisms.

Tue Sep 01 00:00:00 EDT 2015

Contribution and Mobilization of Mesenchymal Stem Cells in a mouse model of carbon tetrachloride-induced liver fibrosis.

Scientific reports

Liu Y,Yang X,Jing Y,Zhang S,Zong C,Jiang J,Sun K,Li R,Gao L,Zhao X,Wu D,Shi Y,Han Z,Wei L

12-8843 was used in Flow cytometry/Cell sorting to provide further evidence to show the role of BM-MSCs in liver fibrosis and to elucidate the mechanism underlying MSCs mobilization in our early liver fibrosis mice model induced by CCl4.

Tue Dec 08 00:00:00 EST 2015

Contribution and Mobilization of Mesenchymal Stem Cells in a mouse model of carbon tetrachloride-induced liver fibrosis.

Scientific reports

Liu Y,Yang X,Jing Y,Zhang S,Zong C,Jiang J,Sun K,Li R,Gao L,Zhao X,Wu D,Shi Y,Han Z,Wei L

Tue Dec 08 00:00:00 EST 2015

Bioinformatics

Protein Aliases: SSEA-4; stage-specific embryonic antigen 4

View more View less

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), PE, eBioscience™

SSEA4 Antibody (12-8843-42) in Flow

Product Details

12-8843-42

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!