Invitrogen

SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), Alexa Fluor™ 488, eBioscience™

FIGURE: 1 / 8

SSEA4 Antibody (53-8843-42) in Flow

Staining of 2102Ep cell line with Mouse IgG3 Isotype Control FITC (Product # 11-4742-42) (open histogram) or Anti-Human SSEA-4 Alexa Fluor® 488 (filled histogram). Total viable cells were used for analysis.

SSEA4 Antibody in Immunocytochemistry (ICC/IF)

SSEA4 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

53-8843-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Dog, Chicken, Human, Mouse, Non-human primate, Rabbit

Published species

Human, Mouse

Host/Isotype

Mouse / IgG3

Class

Monoclonal

Type

Antibody

Clone

eBioMC-813-70 (MC-813-70)

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10609335

Product Specific Information

Description: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells.

The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys.

Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Neighbor-specific gene expression revealed from physically interacting cells during mouse embryonic development.

Proceedings of the National Academy of Sciences of the United States of America

Kim J,Rothová MM,Madan E,Rhee S,Weng G,Palma AM,Liao L,David E,Amit I,Hajkarim MC,Vudatha V,Gutiérrez-García A,Moreno E,Winn R,Trevino J,Fisher PB,Brickman JM,Gogna R,Won KJ

53-8843-42 was used in Flow cytometry/Cell sorting to describe an innovative approach to study contact-specific gene regulation during embryogenesis.

Tue Jan 10 00:00:00 EST 2023

Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals.

Stem cell research

Xu X,Pradhan M,Xu M,Cheng YS,Beers J,Linask KL,Lin Y,Zheng W,Zou J

Published figure using SSEA4 monoclonal antibody (Product # 53-8843-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals.

Stem cell research

Xu X,Pradhan M,Xu M,Cheng YS,Beers J,Linask KL,Lin Y,Zheng W,Zou J

Published figure using SSEA4 monoclonal antibody (Product # 53-8843-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

An induced pluripotent stem cell line (TRNDi001-D) from a Niemann-Pick disease type C1 (NPC1) patient carrying a homozygous p. I1061T (c. 3182T>C) mutation in the NPC1 gene.

Stem cell research

Li R,Pradhan M,Xu M,Roeder A,Beers J,Zou J,Liu C,Porter FD,Zheng W

53-8843 was used in Flow cytometry/Cell sorting to show that this NPC1 iPSC line offers a useful resource for disease modeling and drug development.

Wed Apr 01 00:00:00 EDT 2020

Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates.

Cell reports

Patananan AN,Sercel AJ,Wu TH,Ahsan FM,Torres A,Kennedy SAL,Vandiver A,Collier AJ,Mehrabi A,Van Lew J,Zakin L,Rodriguez N,Sixto M,Tadros W,Lazar A,Sieling PA,Nguyen TL,Dawson ER,Braas D,Golovato J,Cisneros L,Vaske C,Plath K,Rabizadeh S,Niazi KR,Chiou PY,Teitell MA

Published figure using SSEA4 monoclonal antibody (Product # 53-8843-42) in Immunocytochemistry

Tue Dec 29 00:00:00 EST 2020

The Atrioventricular Junction: A Potential Niche Region for Progenitor Cells in the Adult Human Heart.

Stem cells and development

Vukusic K,Sandstedt M,Jonsson M,Jansson M,Oldfors A,Jeppsson A,Dellgren G,Lindahl A,Sandstedt J

Published figure using SSEA4 monoclonal antibody (Product # 53-8843-42) in Immunofluorescence

Thu Aug 15 00:00:00 EDT 2019

Generation of an induced pluripotent stem cell line (TRNDi004-I) from a Niemann-Pick disease type B patient carrying a heterozygous mutation of p.L43_A44delLA in the SMPD1 gene.

Stem cell research

Baskfield A,Li R,Beers J,Zou J,Liu C,Zheng W

53-8843 was used in Flow cytometry/Cell sorting to show that this iPSC line offers a useful resource to study the disease pathophysiology and as a cell-based model for drug development to treat NPB.

Wed May 01 00:00:00 EDT 2019

An induced pluripotent stem cell line (TRNDi006-A) from a MPS IIIB patient carrying homozygous mutation of p.Glu153Lys in the NAGLU gene.

Stem cell research

Huang W,Xu M,Li R,Baskfield A,Kouznetsova J,Beers J,Zou J,Liu C,Zheng W

Published figure using SSEA4 monoclonal antibody (Product # 53-8843-42) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

Generation of an induced pluripotent stem cell line (TRNDi008-A) from a Hunter syndrome patient carrying a hemizygous 208insC mutation in the IDS gene.

Stem cell research

Hong J,Xu M,Li R,Cheng YS,Kouznetsova J,Beers J,Liu C,Zou J,Zheng W

Published figure using SSEA4 monoclonal antibody (Product # 53-8843-42) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

Generation of two induced pluripotent stem cell lines (NHLBIi001-A and NHLBIi001-B) from a healthy Caucasian female volunteer with normal cardiac function.

Stem cell research

Patterson K,Beers J,Linask KL,Lin Y,Hassanzadeh S,Sack MN,Zou J

53-8843 was used in Flow cytometry/Cell sorting to develop two wild-type human-derived induced pluripotent cell lines from a PBMC sample of a patient with normal cardiac function.

Sun Dec 01 00:00:00 EST 2019

Generation of an induced pluripotent stem cell line (TRNDi003-A) from a Noonan syndrome with multiple lentigines (NSML) patient carrying a p.Q510P mutation in the PTPN11 gene.

Stem cell research

Li R,Baskfield A,Lin Y,Beers J,Zou J,Liu C,Jaffré F,Roberts AE,Ottinger EA,Kontaridis MI,Zheng W

53-8843 was used in Flow cytometry/Cell sorting to develop a human induced pluripotent stem cell line from peripheral blood mononuclear cells of a patient with Noonan syndrome with multiple lentigines.

Tue Jan 01 00:00:00 EST 2019

Simplified Footprint-Free Cas9/CRISPR Editing of Cardiac-Associated Genes in Human Pluripotent Stem Cells.

Stem cells and development

Kondrashov A,Duc Hoang M,Smith JGW,Bhagwan JR,Duncan G,Mosqueira D,Munoz MB,Vo NTN,Denning C

Published figure using SSEA4 monoclonal antibody (Product # 53-8843-42) in Flow Cytometry

Thu Mar 15 00:00:00 EDT 2018

Overcoming reprogramming resistance of Fanconi anemia cells.

Blood

Müller LU,Milsom MD,Harris CE,Vyas R,Brumme KM,Parmar K,Moreau LA,Schambach A,Park IH,London WB,Strait K,Schlaeger T,Devine AL,Grassman E,D'Andrea A,Daley GQ,Williams DA

53-8843 was used in Immunofluorescence to identify and overcome mechanisms of resistance to the reprogramming of somatic cells defective in the Fanconi anaemia pathway.

Thu Jun 07 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: SSEA-4; stage-specific embryonic antigen 4

View more View less

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), Alexa Fluor™ 488, eBioscience™

SSEA4 Antibody (53-8843-42) in Flow

Product Details

53-8843-42

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!