Proteintech

ST2 Polyclonal Antibody

ST2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

ST2 Antibody (11920-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 11920-1-AP ( ST2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Product Image Gallery

Product Details

11920-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 12 publications 12 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 10 publications 10 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ST2 Fusion Protein Ag2555 (4-328 aa encoded by BC030975)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognizes the 80 kDa, 40 kDa, and 30 kDa forms in different tissues and cells.

Immunogen sequence: WILAILTIL MYSTAAKFSK QSWGLENEAL IVRCPRQGKP SYTVDWYYSQ TNKSIPTQER NRVFASGQLL KFLPAAVADS GIYTCIVRSP TFNRTGYANV TIYKKQSDCN VPDYLMYSTV SGSEKNSKIY CPTIDLYNWT APLEWFKNCQ ALQGSRYRAH KSFLVIDNVM TEDAGDYTCK FIHNENGANY SVTATRSFTV KDEQGFSLFP VIGAPAQNEI KEVEIGKNAN LTCSACFGKG TQFLAAVLWQ LNGTKITDFG EPRIQQEEGQ NQSFSNGLAC LDMVLRIADV KEEDLLLQYD CLALNLHGLR RHTVRLSRKN PSKECF (4-328 aa encoded by B C030975)

Target Information

IL-33R, also known as ST2, is a receptor for IL-33. It is a member of the IL-1 receptor family of innate receptors. Its extracellular domain consists of 3 Ig-like domains that directly interact with its co-receptor IL-1RAcP, and the ligand alarmin, IL-33. IL-33 ligation results in the association of IL-33R complex with its adaptor proteins MyD88, IRAK1, IRAK4 and TRAF6, and activation of the NF-kB, and MAPK pathways. This, in turn, instigates the release of chemokines and cytokines such as IL-5, IL-6, IL-8, and IL-13. Two most common isoforms of IL-33R that result from alternative splicing are ST2L - the membrane bound form, and ST2S - the soluble form. The membrane bound form can be released from the cell surface by proteolytic cleavage. The soluble forms have been suggested to act as decoys dampening the IL-33 signaling. IL-33R is primarily expressed by mast cells, basophils, and eosinophils. In mouse, IL-33R expression has been shown on ILC2 and Th2 cells. IL-33R signaling has been implicated in the number of immune conditions, including allergies, arthritis, atherosclerosis and sepsis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS.

Shock (Augusta, Ga.)

Zou L,Dang W,Tao Y,Zhao H,Yang B,Xu X,Li Y

Thu Jun 01 00:00:00 EDT 2023

Clinicopathological and Prognostic Relevance of Tumoral Suppression of Tumorigenicity 2 Expression in Patients With Surgically Resected Pancreatic Carcinoma.

Cancer diagnosis & prognosis

Nagase M,Ibuki E,Haba R,Okano K,Kadota K

Sun May 14 00:00:00 EDT 2023

Effect of three clinical therapies on cytokines modulation in the hip articular cartilage and bone improvement in rat early osteonecrosis of the femoral head.

Biomedical journal

Hsu SL,Jhan SW,Hsu CC,Wu YN,Wu KLH,Kuo CA,Chiu HW,Cheng JH

Fri Dec 01 00:00:00 EST 2023

Silencing of microRNA-106b-5p prevents doxorubicin-mediated cardiotoxicity through modulation of the PR55α/YY1/sST2 signaling axis.

Molecular therapy. Nucleic acids

Lax A,Soler F,Fernandez Del Palacio MJ,Pascual-Oliver S,Ballester MR,Fuster JJ,Pascual-Figal D,Asensio-Lopez MDC

Tue Jun 13 00:00:00 EDT 2023

IL-33/ST2 axis promotes remodeling of the extracellular matrix and drives protective microglial responses in the mouse model of perioperative neurocognitive disorders.

International immunopharmacology

Li S,Liu H,Qian Y,Jiang L,Liu S,Liu Y,Liu C,Gu X

Sun Jan 01 00:00:00 EST 2023

Multifunctional hydrogel modulates the immune microenvironment to improve allogeneic spinal cord tissue survival for complete spinal cord injury repair.

Acta biomaterialia

Gao X,You Z,Li Y,Kang X,Yang W,Wang H,Zhang T,Zhao X,Sun Y,Shen H,Dai J

Sun Jan 01 00:00:00 EST 2023

Unveiling IL-33/ST2 Pathway Unbalance in Cardiac Remodeling Due to Obesity in Zucker Fatty Rats.

International journal of molecular sciences

Sitzia C,Vianello E,Dozio E,Kalousová M,Zima T,Brizzola S,Roccabianca P,Tedeschi G,Lamont J,Tacchini L,Corsi-Romanelli MM

Thu Jan 19 00:00:00 EST 2023

Extracellular ATP accelerates cell death and decreases tight junction protein ZO-1 in hypoxic cochlear strial marginal cells in neonatal rats.

Cellular signalling

Zhao Y,Huang S,Xie R,Liu J

Tue Aug 01 00:00:00 EDT 2023

Quercetin Ameliorates Renal Injury and Pyroptosis in Lupus Nephritis through Inhibiting IL-33/ST2 Pathway In Vitro and In Vivo.

Antioxidants (Basel, Switzerland)

Chen HY,Chiang YF,Hong YH,Shieh TM,Huang TC,Ali M,Chang HY,Wang KL,Hsia SM

Sun Nov 13 00:00:00 EST 2022

Differential expression of CD8 defines phenotypically distinct cytotoxic T cells in cancer and multiple sclerosis.

Clinical and translational medicine

Burkard T,Herrero San Juan M,Dreis C,Kiprina A,Namgaladze D,Siebenbrodt K,Luger S,Foerch C,Pfeilschifter JM,Weigert A,Radeke HH

Thu Dec 01 00:00:00 EST 2022

Extracorporeal Shockwave Therapy Modulates the Expressions of Proinflammatory Cytokines IL33 and IL17A, and Their Receptors ST2 and IL17RA, within the Articular Cartilage in Early Avascular Necrosis of the Femoral Head in a Rat Model.

Mediators of inflammation

Cheng JH,Jhan SW,Hsu CC,Chiu HW,Hsu SL

Thu Mar 31 00:00:00 EDT 2022

Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3.

Journal of neuroinflammation