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Invitrogen
STAT6 Recombinant Rabbit Monoclonal Antibody (21HCLC)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
STAT6 Antibody (701110) in ICC/IF
Immunofluorescent analysis of STAT6 in HeLa cells using a STAT6 recombinant rabbit monoclonal antibody (Product # 701110) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic localization of STAT6.
Product Details
701110
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
21HCLC
Immunogen
Peptide corresponding to amino acids 630-638 of human singal transducer and activator of transcription 6
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532393
Product Specific Information
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
STAT6 protein is a transcription factor activated by cytokines, particularly interleukin-4 and IL13. (Stat6-/-) were found to be deficient in IL-4-mediated functions including Th2 helper T-cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. STAT6 is critically involved in Th2 immune response. STAT6 is crucial for IL-4-mediated biological responses, and naturally in two isoforms: STAT6a and STAT6c.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 12S1644; IL-4 Stat; IL4 STAT; Signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; Signal transducer and transcription activator 6; STAT; STAT, interleukin4-induced; STAT-6; transcription factor IL-4 STAT
Gene Aliases: D12S1644; IL-4-STAT; STAT6; STAT6B; STAT6C
UniProt ID: (Human) P42226, (Mouse) P52633
Entrez Gene ID: (Human) 6778, (Mouse) 20852
negative regulation of transcription from RNA polymerase II promoter
T-helper 1 cell lineage commitment
negative regulation of type 2 immune response
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
signal transduction
positive regulation of type I interferon production
mammary gland epithelial cell proliferation
interleukin-4-mediated signaling pathway
regulation of cell proliferation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgE isotypes
mammary gland morphogenesis
cellular response to hydrogen peroxide
cellular response to reactive nitrogen species
regulation of transcription, DNA-templated
cytokine-mediated signaling pathway
response to cytokine
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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