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Invitrogen
STRO-1 Monoclonal Antibody (STRO-1), eBioscience™
Product Details
14-6688-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Rat
Published species
Dog,
Human,
Rat
Host/Isotype
Mouse
/ IgM, lambda
Class
Monoclonal
Type
Antibody
Clone
STRO-1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572902
Product Specific Information
Description: The monoclonal antibody STRO-1 recognizes STRO-1, a bone marrow (immature) mesenchymal cell surface protein. Pluripotent stromal cells can give rise to several lineages: osteoblasts and chondrocytes, which form bone, adipocytes or hematopoietic cells. STRO-1+ cells when co-transplanted with hematopoietic stem cells have been shown to reduce the occurrence of graft versus host disease. In addition, in vitro cultures of Stro-1+ cells along with CD34+ cells give rise to CFUs (colony forming units) composed of hematopoietic precursors, establishing a supporting role of these mesenchymal cells in blood cell development.
The STRO-1 antibody has been reported to recognize both bovine and rat Stro-1 protein.
Applications Reported: This STRO-1 antibody has been reported for use in flow cytometric analysis, immunocytochemistry, and immunohistochemical staining of frozen tissue sections.
Applications Tested: This STRO-1 antibody has been tested by immunocytochemistry on methanol-fixed MG-63 cells and can be used at less than or equal to 20 µg/mL. The STRO-1 antibody has also been tested by flow cytometry on MG-63 cells and can be used at less than or equal to 0.5 µg/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow. STRO-1 binds to approximately 10% of bone marrow mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage (CFU-GM), erythroid bursts (BFU-E), and mixed colonies (CFU-Mix). From bone marrow cells, the frequency of fibroblast colony forming cells (CFU-F) is enriched approximately 100-fold in the STRO-1-positive/Glycophorin A-negative population than in the STRO-1-positive/Glycophorin A-positive population. When plated under long-term BM culture conditions, STRO-1-positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, and fibroblastic elements. A STRO-1-positive enriched subset of marrow cells is capable of differentiating into multiple mesenchymal lineages including hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype, adipocytes, osteoblasts and chondrocytes. STRO-1 is a valuable antibody for the identification, isolation and functional characterization of human bone marrow stromal cell precursors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: STRO1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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