Proteintech

STT3B Polyclonal Antibody

STT3B Antibody in Immunocytochemistry (ICC/IF)

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STT3B Antibody (15323-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 15323-1-AP (STT3B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

STT3B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

15323-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 12 publications 12 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

STT3B Fusion Protein Ag2039 (1-225 aa encoded by BC015880)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.38 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSWWDYGYQ IAGMANRTTL VDNNTWNNSH IALVGKAMSS NETAAYKIMR TLDVDYVLVI FGGVIGYSGD DINKFLWMVR IAEGEHPKDI RESDYFTPQG EFRVDKAGSP TLLNCLMYKM SYYRFGEMQL DFRTPPGFDR TRNAEIGNKD IKFKHLEEAF TSEHWLVRIY KVKAPDNRET LDHKPRVTNI FPKQKYLSKK TTKRKRGYIK NKLVFKKGKK IFKKTV (1-225 aa encoded by B C015880)

Target Information

Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. This subunit contains the active site and the acceptor peptide and donor lipid-linked oligosaccharide (LLO) binding pockets. STT3B is present in a small subset of OST complexes and mediates both cotranslational and post-translational N-glycosylation of target proteins: STT3B-containing complexes are required for efficient post-translational glycosylation and while they are less competent than STT3A-containing complexes for cotranslational glycosylation, they have the ability to mediate glycosylation of some nascent sites that are not accessible for STT3A. STT3B-containing complexes also act post-translationally and mediate modification of skipped glycosylation sites in unfolded proteins. Plays a role in ER-associated degradation (ERAD) pathway that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins by mediating N-glycosylation of unfolded proteins, which are then recognized by the ERAD pathway and targeted for degradation. Mediates glycosylation of the disease variant AMYL-TTR 'Asp-38' of TTR at 'Asn-118', leading to its degradation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Signal recognition particle receptor-β (SR-β) coordinates cotranslational N-glycosylation.

Science advances

Phoomak C,Rinis N,Baro M,Shrimal S,Bennett D,Shaffer SA,Lehrman M,Gilmore R,Contessa JN

Fri Mar 17 00:00:00 EDT 2023

MITA oligomerization upon viral infection is dependent on its N-glycosylation mediated by DDOST.

PLoS pathogens

Tu Y,Yin XJ,Liu Q,Zhang S,Wang J,Ji BZ,Zhang J,Sun MS,Yang Y,Wang CH,Yin L,Liu Y

Tue Nov 01 00:00:00 EDT 2022

Targeting conserved N-glycosylation blocks SARS-CoV-2 variant infection in vitro.

EBioMedicine

Huang HC,Lai YJ,Liao CC,Yang WF,Huang KB,Lee IJ,Chou WC,Wang SH,Wang LH,Hsu JM,Sun CP,Kuo CT,Wang J,Hsiao TC,Yang PJ,Lee TA,Huang W,Li FA,Shen CY,Lin YL,Tao MH,Li CW

Wed Dec 01 00:00:00 EST 2021

Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis.

Molecular & cellular proteomics : MCP

Wright MT,Kouba L,Plate L

Mon Mar 14 00:00:00 EDT 2022

N-Glycosylation Regulates Chitinase 3-like-1 and IL-13 Ligand Binding to IL-13 Receptor α2.

American journal of respiratory cell and molecular biology

He CH,Lee CG,Ma B,Kamle S,Choi AMK,Elias JA

Tue Sep 01 00:00:00 EDT 2020

Comparative proteomic analyses of Duchenne muscular dystrophy and Becker muscular dystrophy muscles: changes contributing to preserve muscle function in Becker muscular dystrophy patients.

Journal of cachexia, sarcopenia and muscle

Capitanio D,Moriggi M,Torretta E,Barbacini P,De Palma S,Viganò A,Lochmüller H,Muntoni F,Ferlini A,Mora M,Gelfi C

Wed Apr 01 00:00:00 EDT 2020

Editing N-Glycan Site Occupancy with Small-Molecule Oligosaccharyltransferase Inhibitors.

Cell chemical biology

Rinis N,Golden JE,Marceau CD,Carette JE,Van Zandt MC,Gilmore R,Contessa JN

Thu Oct 18 00:00:00 EDT 2018

Editing N-Glycan Site Occupancy with Small-Molecule Oligosaccharyltransferase Inhibitors.

Cell chemical biology

Rinis N,Golden JE,Marceau CD,Carette JE,Van Zandt MC,Gilmore R,Contessa JN

Thu Oct 18 00:00:00 EDT 2018

Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.

mBio

Lin DL,Cherepanova NA,Bozzacco L,MacDonald MR,Gilmore R,Tai AW

Tue Jul 18 00:00:00 EDT 2017

Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.

mBio

Lin DL,Cherepanova NA,Bozzacco L,MacDonald MR,Gilmore R,Tai AW

Tue Jul 18 00:00:00 EDT 2017

TCA cycle rewiring fosters metabolic adaptation to oxygen restriction in skeletal muscle from rodents and humans.

Scientific reports

Capitanio D,Fania C,Torretta E,Viganò A,Moriggi M,Bravatà V,Caretti A,Levett DZH,Grocott MPW,Samaja M,Cerretelli P,Gelfi C

Tue Aug 29 00:00:00 EDT 2017

TCA cycle rewiring fosters metabolic adaptation to oxygen restriction in skeletal muscle from rodents and humans.

Scientific reports

Capitanio D,Fania C,Torretta E,Viganò A,Moriggi M,Bravatà V,Caretti A,Levett DZH,Grocott MPW,Samaja M,Cerretelli P,Gelfi C

Tue Aug 29 00:00:00 EDT 2017

Changes in cellular glycosylation of leukemia cells upon treatment with acridone derivatives yield insight into drug action.

Proteomics

Wang Y,Park D,Galermo AG,Gao D,Liu H,Lebrilla CB

Thu Dec 01 00:00:00 EST 2016

Changes in cellular glycosylation of leukemia cells upon treatment with acridone derivatives yield insight into drug action.

Proteomics

Wang Y,Park D,Galermo AG,Gao D,Liu H,Lebrilla CB

Thu Dec 01 00:00:00 EST 2016

Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.

Nature chemical biology

Lopez-Sambrooks C,Shrimal S,Khodier C,Flaherty DP,Rinis N,Charest JC,Gao N,Zhao P,Wells L,Lewis TA,Lehrman MA,Gilmore R,Golden JE,Contessa JN

Thu Dec 01 00:00:00 EST 2016

TUSC3 loss alters the ER stress response and accelerates prostate cancer growth in vivo.

Scientific reports

Horak P,Tomasich E,Vaňhara P,Kratochvílová K,Anees M,Marhold M,Lemberger CE,Gerschpacher M,Horvat R,Sibilia M,Pils D,Krainer M

Fri Jan 17 00:00:00 EST 2014

TUSC3 loss alters the ER stress response and accelerates prostate cancer growth in vivo.

Scientific reports

Horak P,Tomasich E,Vaňhara P,Kratochvílová K,Anees M,Marhold M,Lemberger CE,Gerschpacher M,Horvat R,Sibilia M,Pils D,Krainer M

Fri Jan 17 00:00:00 EST 2014

TUSC3 loss alters the ER stress response and accelerates prostate cancer growth in vivo.

Scientific reports

Horak P,Tomasich E,Vaňhara P,Kratochvílová K,Anees M,Marhold M,Lemberger CE,Gerschpacher M,Horvat R,Sibilia M,Pils D,Krainer M

Fri Jan 17 00:00:00 EST 2014

TUSC3 loss alters the ER stress response and accelerates prostate cancer growth in vivo.

Scientific reports

Horak P,Tomasich E,Vaňhara P,Kratochvílová K,Anees M,Marhold M,Lemberger CE,Gerschpacher M,Horvat R,Sibilia M,Pils D,Krainer M

Fri Jan 17 00:00:00 EST 2014

Bioinformatics

Protein Aliases: B6dom1 antigen; dolichyl-diphosphooligosaccharide protein glycotransferase; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B; homolog of yeast STT3; Oligosaccharyl transferase subunit STT3B; Source of immunodominant MHC-associated peptides; Source of immunodominant MHC-associated peptides homolog; STT3, subunit of the oligosaccharyltransferase complex, homolog B; STT3-B; STT3B, subunit of the oligosaccharyltransferase complex (catalytic)

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Gene Aliases: 1300006C19Rik; CDG1X; RGD1311563; SIMP; STT3-B; STT3B

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UniProt ID: (Human) Q8TCJ2, (Mouse) Q3TDQ1

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Entrez Gene ID: (Human) 201595, (Rat) 363160, (Mouse) 68292

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Function(s)

dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyl transferase activity transferase activity glycosyltransferase transferase metabolite interconversion enzyme

Process(es)

glycoprotein catabolic process response to unfolded protein protein N-linked glycosylation via asparagine ER-associated ubiquitin-dependent protein catabolic process co-translational protein modification post-translational protein modification protein glycosylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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STT3B Polyclonal Antibody

STT3B Antibody (15323-1-AP) in ICC/IF

Product Details

15323-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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