Proteintech

STUB1 Polyclonal Antibody

STUB1 Antibody in Immunocytochemistry (ICC/IF)

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STUB1 Antibody (55430-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed MCF-7 cells using 55430-1-AP (STUB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

STUB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

55430-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.36 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific to STUB1.

Target Information

A recently identified protein, termed carboxyl terminus of hsc70-interacting protein (CHIP), has been shown to interact both with the constitutive form of hsc70 and the stress inducible form, hsp70. This novel 35 kDa cytoplasmic protein has been shown to be highly expressed in striated muscle in vivo. Additional studies have shown that this protein is expressed over a broad range of cultured tissues. Through immunoprecipitation experiments, CHIP has been shown to directly bind to the carboxyl terminus of hsc70 and hsp70 where it decreases ATPase activity and reduces overall chaperone efficiency. CHIP has also been identified as an important protein in the ubiquitin-proteasome system. CHIP contains a U-box domain and acts as an E3 ubiquitin-ligase in conjunction with hsc70 and hsp90.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

STUB1 regulates antiviral RNAi through inducing ubiquitination and degradation of Dicer and AGO2 in mammals.

Virologica Sinica

Zhang S,Zhang X,Bie Y,Kong J,Wang A,Qiu Y,Zhou X

Mon Aug 01 00:00:00 EDT 2022

ZC3H15 promotes glioblastoma progression through regulating EGFR stability.

Cell death & disease

Hou J,Xu M,Gu H,Pei D,Liu Y,Huang P,Chang H,Cui H

Thu Jan 13 00:00:00 EST 2022

PKM2 compensates for proteasome dysfunction by mediating the formation of the CHIP-HSP70-BAG3 complex and the aggregation of ubiquitinated proteins.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang C,Tang Q,Xia H,Xu H,Bi F

Sat Jan 01 00:00:00 EST 2022

PKM2 compensates for proteasome dysfunction by mediating the formation of the CHIP-HSP70-BAG3 complex and the aggregation of ubiquitinated proteins.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang C,Tang Q,Xia H,Xu H,Bi F

Sat Jan 01 00:00:00 EST 2022

CHIP Decline Is Associated With Isoflurane-Induced Neurodegeneration in Aged Mice.

Frontiers in neuroscience

Xu Q,Xiong J,Xu L,Wu Y,Li M,Li Q,Jiang T,Luo A,Zhang Y

Tue Apr 05 00:00:00 EDT 2022

Regulation of STUB1 expression and its biological significance in mouse Sertoli cells.

Systems biology in reproductive medicine

Li T,Zheng C,Han WJ,Chen ZZ

Mon Aug 01 00:00:00 EDT 2022

Regulation of STUB1 expression and its biological significance in mouse Sertoli cells.

Systems biology in reproductive medicine

Li T,Zheng C,Han WJ,Chen ZZ

Mon Aug 01 00:00:00 EDT 2022

Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover.

Nature communications

Martin TG,Myers VD,Dubey P,Dubey S,Perez E,Moravec CS,Willis MS,Feldman AM,Kirk JA

Wed May 19 00:00:00 EDT 2021

CHIP protects against MPP⁺/MPTP-induced damage by regulating Drp1 in two models of Parkinson's disease.

Aging

Hu Z,Mao C,Wang H,Zhang Z,Zhang S,Luo H,Tang M,Yang J,Yuan Y,Wang Y,Liu Y,Fan L,Zhang Q,Yao D,Liu F,Schisler JC,Shi C,Xu Y

Sat Jan 02 00:00:00 EST 2021

Network-Based Expression Analyses and Experimental Verifications Reveal the Involvement of STUB1 in Acute Kidney Injury.

Frontiers in molecular biosciences

Shi Y,Chen G,Teng J

Fri Jul 16 00:00:00 EDT 2021

Network-Based Expression Analyses and Experimental Verifications Reveal the Involvement of STUB1 in Acute Kidney Injury.

Frontiers in molecular biosciences

Shi Y,Chen G,Teng J

Fri Jul 16 00:00:00 EDT 2021

Network-Based Expression Analyses and Experimental Verifications Reveal the Involvement of STUB1 in Acute Kidney Injury.

Frontiers in molecular biosciences

Shi Y,Chen G,Teng J

Fri Jul 16 00:00:00 EDT 2021

Network-Based Expression Analyses and Experimental Verifications Reveal the Involvement of STUB1 in Acute Kidney Injury.

Frontiers in molecular biosciences

Shi Y,Chen G,Teng J

Fri Jul 16 00:00:00 EDT 2021

A nonautophagic role of ATG5 in regulating cell growth by targeting c-Myc for proteasome-mediated degradation.

iScience

Li S,Zhang L,Zhang G,Shangguan G,Hou X,Duan W,Xi Y,Xu N,Zhang B,Dong J,Wang Y,Cui W,Chen S

Fri Nov 19 00:00:00 EST 2021

Upregulation of C Terminus of Hsc70-Interacting Protein Attenuates Apoptosis and Procoagulant Activity and Facilitates Brain Repair After Traumatic Brain Injury.

Frontiers in neuroscience

Chen H,Jing Y,Xu Z,Yang D,Ju S,Guo Y,Tian H,Xue L

Wed Nov 29 00:00:00 EST 2023

TRIM16 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Modulating CHIP-Mediated Degradation of RUNX2.

Frontiers in cell and developmental biology

Zhao Y,Zhai Q,Liu H,Xi X,Chen S,Liu D

Tue Jan 26 00:00:00 EST 2021

The E3 ubiquitin ligase STUB1 attenuates cell senescence by promoting the ubiquitination and degradation of the core circadian regulator BMAL1.

The Journal of biological chemistry

Ullah K,Chen S,Lu J,Wang X,Liu Q,Zhang Y,Long Y,Hu Z,Xu G

Fri Apr 03 00:00:00 EDT 2020

Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation.

Molecular cancer

Xie M,Yu T,Jing X,Ma L,Fan Y,Yang F,Ma P,Jiang H,Wu X,Shu Y,Xu T

Mon Jun 29 00:00:00 EDT 2020

DpdtbA-Induced Growth Inhibition in Human Esophageal Cancer Cells Involved Inactivation of the p53/EGFR/AKT Pathway.

Oxidative medicine and cellular longevity

Wang Z,Li C,Li Y,Guo X,Yan Z,Gao F,Li C

Fri Feb 21 00:00:00 EST 2020

Anisomycin prevents OGD-induced necroptosis by regulating the E3 ligase CHIP.

Scientific reports

Tang MB,Li YS,Li SH,Cheng Y,Zhang S,Luo HY,Mao CY,Hu ZW,Schisler JC,Shi CH,Xu YM

Mon Apr 23 00:00:00 EDT 2018

Anisomycin prevents OGD-induced necroptosis by regulating the E3 ligase CHIP.

Scientific reports

Tang MB,Li YS,Li SH,Cheng Y,Zhang S,Luo HY,Mao CY,Hu ZW,Schisler JC,Shi CH,Xu YM

Mon Apr 23 00:00:00 EDT 2018

Anisomycin prevents OGD-induced necroptosis by regulating the E3 ligase CHIP.

Scientific reports

Tang MB,Li YS,Li SH,Cheng Y,Zhang S,Luo HY,Mao CY,Hu ZW,Schisler JC,Shi CH,Xu YM

Mon Apr 23 00:00:00 EDT 2018

Anisomycin prevents OGD-induced necroptosis by regulating the E3 ligase CHIP.

Scientific reports

Tang MB,Li YS,Li SH,Cheng Y,Zhang S,Luo HY,Mao CY,Hu ZW,Schisler JC,Shi CH,Xu YM

Mon Apr 23 00:00:00 EDT 2018

The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma.

Nature communications

Li Z,Zhang J,Liu X,Li S,Wang Q,Hu Z,Yu T,Ding J,Li J,Yao M,Fan J,Huang S,Gao Q,Zhao Y,He X

Fri Apr 20 00:00:00 EDT 2018

The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma.

Nature communications

Li Z,Zhang J,Liu X,Li S,Wang Q,Hu Z,Yu T,Ding J,Li J,Yao M,Fan J,Huang S,Gao Q,Zhao Y,He X

Fri Apr 20 00:00:00 EDT 2018

Enhanced tenogenic differentiation and tendon-like tissue formation by CHIP overexpression in tendon-derived stem cells.

Acta biochimica et biophysica Sinica

Han W,Chen L,Liu J,Guo A

Sat Apr 01 00:00:00 EDT 2017

Enhanced tenogenic differentiation and tendon-like tissue formation by CHIP overexpression in tendon-derived stem cells.

Acta biochimica et biophysica Sinica

Han W,Chen L,Liu J,Guo A

Sat Apr 01 00:00:00 EDT 2017

CHIP promotes thyroid cancer proliferation via activation of the MAPK and AKT pathways.

Biochemical and biophysical research communications

Zhang L,Liu L,He X,Shen Y,Liu X,Wei J,Yu F,Tian J

Fri Aug 26 00:00:00 EDT 2016

CHIP promotes thyroid cancer proliferation via activation of the MAPK and AKT pathways.

Biochemical and biophysical research communications

Zhang L,Liu L,He X,Shen Y,Liu X,Wei J,Yu F,Tian J

Fri Aug 26 00:00:00 EDT 2016

CHIP promotes thyroid cancer proliferation via activation of the MAPK and AKT pathways.

Biochemical and biophysical research communications

Zhang L,Liu L,He X,Shen Y,Liu X,Wei J,Yu F,Tian J

Fri Aug 26 00:00:00 EDT 2016

CHIP promotes thyroid cancer proliferation via activation of the MAPK and AKT pathways.

Biochemical and biophysical research communications

Zhang L,Liu L,He X,Shen Y,Liu X,Wei J,Yu F,Tian J

Fri Aug 26 00:00:00 EDT 2016

Dopamine controls systemic inflammation through inhibition of NLRP3 inflammasome.

Cell

Yan Y,Jiang W,Liu L,Wang X,Ding C,Tian Z,Zhou R

Thu Jan 15 00:00:00 EST 2015

Dopamine controls systemic inflammation through inhibition of NLRP3 inflammasome.

Cell

Yan Y,Jiang W,Liu L,Wang X,Ding C,Tian Z,Zhou R

Thu Jan 15 00:00:00 EST 2015

Bioinformatics

Protein Aliases: Antigen NY-CO-7; Carboxy terminus of Hsp70-interacting protein; CLL-associated antigen KW-8; E3 ubiquitin-protein ligase CHIP; heat shock protein A binding protein 2 (c-terminal); LA16c-313D11.6; RING-type E3 ubiquitin transferase CHIP; serologically defined colon cancer antigen 7; STIP1 homology and U box-containing protein 1; STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase

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Gene Aliases: 0610033N24Rik; 2210017D18Rik; 2310040B03Rik; AW046544; CHIP; HSPABP2; NY-CO-7; PP1131; SCAR16; SDCCAG7; STUB1; UBOX1

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UniProt ID: (Human) Q9UNE7, (Mouse) Q9WUD1, (Rat) A6HD62

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Entrez Gene ID: (Human) 10273, (Mouse) 56424, (Rat) 287155

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Function(s)

G-protein coupled receptor binding ubiquitin-protein transferase activity protein binding ligase activity enzyme binding kinase binding Hsp70 protein binding protein binding, bridging TPR domain binding ubiquitin protein ligase binding ubiquitin-ubiquitin ligase activity protein homodimerization activity SMAD binding misfolded protein binding Hsp90 protein binding ubiquitin protein ligase activity ubiquitin protein ligase activity involved in ERAD pathway UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity heat shock protein binding coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase

Process(es)

protein polyubiquitination DNA repair ubiquitin-dependent protein catabolic process misfolded or incompletely synthesized protein catabolic process protein ubiquitination negative regulation of transforming growth factor beta receptor signaling pathway ubiquitin-dependent SMAD protein catabolic process endoplasmic reticulum unfolded protein response positive regulation of protein ubiquitination regulation of protein stability regulation of glucocorticoid metabolic process negative regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process ERAD pathway ERBB2 signaling pathway protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of ubiquitin-protein transferase activity protein maturation protein autoubiquitination protein K63-linked ubiquitination cellular response to misfolded protein positive regulation of chaperone-mediated protein complex assembly protein folding cellular response to DNA damage stimulus

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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STUB1 Polyclonal Antibody

STUB1 Antibody (55430-1-AP) in ICC/IF

Product Details

55430-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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