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SULF1 Antibody (PA5-115984) in ICC/IF
Immunocytochemistry analysis of SULF1 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115984) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115984
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SULF1(Accession Q8IWU6), corresponding to amino acid residues L412-Q462.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900618
Product Specific Information
Antibody detects endogenous levels of total Sulfatase 1.
Target Information
Heparan sulfate proteoglycans act as coreceptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. Heparan sulfate 6-O-endosulfatases, such as SULF1, selectively remove 6-O-sulfate groups from heparan sulfate. This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Arylsulfatase; Extracellular sulfatase Sulf-1; FLJ30905; FLJ38022; FLJ41750; hSulf-1; MSulf-1; N-acetylglucosamine-6-sulfatase; RSulfFP1; Sulfatase FP
Gene Aliases: AI467640; AW121680; KIAA1077; mKIAA1077; SULF-1; SULF1
UniProt ID: (Human) Q8IWU6, (Mouse) Q8K007, (Rat) Q8VI60
Entrez Gene ID: (Human) 23213, (Mouse) 240725, (Rat) 171396
kidney development
negative regulation of endothelial cell proliferation
chondrocyte development
glomerular filtration
apoptotic process
positive regulation of vascular endothelial growth factor production
esophagus smooth muscle contraction
negative regulation of angiogenesis
positive regulation of Wnt signaling pathway
heparan sulfate proteoglycan metabolic process
negative regulation of cell migration
positive regulation of BMP signaling pathway
glomerular basement membrane development
glial cell-derived neurotrophic factor receptor signaling pathway
regulation of fibroblast growth factor receptor signaling pathway
negative regulation of fibroblast growth factor receptor signaling pathway
vascular endothelial growth factor receptor signaling pathway
embryonic skeletal system development
cartilage development
bone development
innervation
negative regulation of prostatic bud formation
sulfur compound metabolic process
metabolic process
cartilage condensation
cell adhesion
limb joint morphogenesis
positive regulation of smooth muscle cell proliferation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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